99 research outputs found
Molecular Cloning of the Left-Terminal SmaI-D Fragment of Adenovirus Type 7 DNA
The left-terminal SmaI-D fragment (map position 0-13.5%) of adenovirus type 7 DNA has been cloned in the plasmid vector pBR322 by the homopolymeric tailings of poly (dG) and poly (dC). Two isolated clones were characterized by mapping the cleavage sites of restriction endonucleases and by sequencing the junction between viral insert and pBR322
Establishment of a Rat Cell Line Carrying Hormone Inducible Human Adenovirus Type 12 E1A Gene
Cleavage Maps of Weakly Oncogenic Human Adenovirus Type 7 DNA by Restriction Endonuclease Barn HI and SalI
Analysis of Viral Sequences Integrated and Expressed in Rat Cell Lines Transformed by the Leftmost 4.7% Fragment of Adenovirus Type 12 DNA
RT-PCR法によるヒトサイトメガロウイルス前初期遺伝子mRNAの検出
We have established a method for detection of human cytomegalovirus (HCMV) immediate early gene (IE)-specific mRNA by reverse transcription polymerase chain reaction (RT-PCR). Using the IE-1 specific primer fragments designed for RT-PCR, we have amplified a 232 base-pair fragment which represents IE-1 mRNA transcribed from the IE-1 gene of HCMV AD169 strain. No DNA cross reactivities to other human herpes virus DNAs or human embryonic lung cell (MRC-5 line) DNA were observed. The RT-PCR assay indicated HCMV IE-1-specific mRNA in HCMV-infected MRC-5 cells and in peripheral blood specimens from one of 3 patients examined. The results indicate that RT-PCR will be readily applicable to rapid detection of HCMV mRNA and may be useful for diagnosis of active primary or recurrent HCMV infections
Potential Activity of Transcriptional Promoter in the Replication Origin Region of Adenovirus type 5 DNA
The size distribution of nuclear and cytoplasmic virus-specific RNA molecules in rat embryo cells transformed by Adenovirus type 2
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