Phylogenetic relationships of GLYI and GLYII from various plant species.

<p>A phylogenetic tree based on the multiple alignments of the GLYI and GLYII amino acid sequences was constructed using MEGA 5.05 software with the Neighbor-Joining method. Bootstrap support from 1,000 reiterations is indicated above the branches. “Br”, “At” and “Os” refer to the GLYI and GLYII proteins in <i>B</i>. <i>rapa</i>, <i>A</i>. <i>thaliana</i> and <i>O sativa</i> (only the first splice variants were considered in the case of multiple members), respectively.</p

RT-qPCR assay of the expression of the representative <i>BrGLYI</i> under Cd and Pb treatments.

<p>The y-axis indicates the relative gene expression; the x-axis indicates the different treatments under the control (CK), Cd and Pb conditions.</p

Summary of the known functions of the <i>GLYI / GLYII</i> genes using a transgenic approach.

<p>Summary of the known functions of the <i>GLYI / GLYII</i> genes using a transgenic approach.</p

Genome-wide analysis and expression profiles of glyoxalase gene families in Chinese cabbage (<i>Brassica rapa</i> L) - Fig 1

<p><b>The positions of the <i>BrGLYI</i> (A) and <i>BrGLYII</i> (B) genes distributed on <i>B</i>. <i>rapa</i> chromosomes.</b> Duplicated glyoxalase genes are connected by black lines between the two relevant chromosomes. The scale is in megabase (Mb). The exact position (Mb) of each glyoxalase gene is shown on the chromosomes. Chromosome numbers are shown at the bottom of each bar.</p

The relative expression levels of the <i>BrGLY</i> genes in response to heavy metal treatment.

<p>The raw data were obtained through GEO series accession number GSE55264 (<a href="http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE55264" target="_blank">http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE55264</a>). The gene expression level changes under Zinc deficiency (ZnD), iron deficiency (FeD), excess Zn (ZnE) and cadmium exposure (CdE) compared to the normal condition were analyzed. (a) The <i>BrGLYI</i> gene expression level, (b) The <i>BrGLYII</i> gene expression level.</p

Expression profiles of the <i>BrGLY</i> genes using hierarchical clustering across various tissues.

<p>(A) The expression profiles of the <i>BrGLYI</i> genes; (B) The expression profiles of the <i>BrGLYII</i> genes. <b>Note:</b> The black color indicates that the gene was not detected in the tissue. The data was obtained from GEO database (<a href="http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE43245" target="_blank">http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE43245</a>).</p

Relative expressions of the <i>BrGLYI</i> genes in different tissues of <i>B</i>. <i>rapa</i> confirmed by RT-qPCR.

<p>The normalized relative quantity in the seed was set as “1”. If the gene did not express in the seed, the expression level of in the stem was set at “1”.</p

List of putative <i>BrGLYI</i> and <i>BrGLYII</i> genes along with gene and protein detailed information.

<p>List of putative <i>BrGLYI</i> and <i>BrGLYII</i> genes along with gene and protein detailed information.</p

Gene structure and phylogenetic relationship of <i>BrGLYI</i> and <i>BrGLYII</i>.

<p>(A) BrGLYI, (B) BrGLYII proteins. An unrooted tree was generated using the Neighbor-Joining method with 1,000 bootstrap by MEGA5.05 software using the full-length amino acid sequences of the sixteen BrGLYI and fifteen BrGLYII proteins. CDS and amino acid sequences of <i>BrGLYI</i> and <i>BrGLYII</i> are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0191159#pone.0191159.s004" target="_blank">S2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0191159#pone.0191159.s005" target="_blank">S3</a> Tables.</p

Identification of homologous <i>GLYI</i> and <i>GLYII</i> genes between <i>A</i>. <i>thaliana</i> and subgenomes in <i>B</i>. <i>rapa</i>.

<p>Identification of homologous <i>GLYI</i> and <i>GLYII</i> genes between <i>A</i>. <i>thaliana</i> and subgenomes in <i>B</i>. <i>rapa</i>.</p