Le Bulletin de Vouziers : journal politique, industriel et agricole de l'arrondissement, paraissant toutes les semaines

14 février 18861886/02/14 (N219)-1886/02/14.Appartient à l’ensemble documentaire : ChArdenn

OCI-AML3 (p53 wild type) or HL–60 (p53 null) cells were treated with DMSO or Nutlin 3a for 72 hours and imaged using transmission electron microscopy.

<p>While OCI-AML3 cells showed numerous electron-dense ‘mitophagic’ vacuoles at 72 hrs of Nutlin 3a treatment, such vacuoles were absent in HL–60 cells (lower panel).</p

OCI-AML3 or REH (p53 wild type) cells stably expressing either control or Beclin1-silencing shRNA were treated with Nutlin 3a, stained with MDC and imaged with confocal microscopy for MDC positive ‘puncta’ representing autophagic vacuoles.

<p>OCI-AML3 or REH (p53 wild type) cells stably expressing either control or Beclin1-silencing shRNA were treated with Nutlin 3a, stained with MDC and imaged with confocal microscopy for MDC positive ‘puncta’ representing autophagic vacuoles.</p

HL60 (A) or OCI-AML3 cells stably expressing shRNA silencing p53 (B) were treated with Nutlin 3a for 24–96 hrs and analyzed by flow cytometry for AnnV/MDC staining.

<p>HL60 (A) or OCI-AML3 cells stably expressing shRNA silencing p53 (B) were treated with Nutlin 3a for 24–96 hrs and analyzed by flow cytometry for AnnV/MDC staining.</p

OCI-AML3 cells transduced with LC3-GFP-mCherry construct were treated with 5 μM Nutlin 3a and visualized by confocal microscopy.

<p>Increase in proportion of red ‘puncta’ at 96 hrs compared to 48 hrs (merge images) in Nutlin 3a treated cells indicate completion of autophagic flux.</p

OCI-AML3 cells were treated with Nutlin 3a for indicated time and Western blots done for p53, LC3B, Atg 5/12, p62.

<p>OCI-AML3 cells were treated with Nutlin 3a for indicated time and Western blots done for p53, LC3B, Atg 5/12, p62.</p

Mouse embryonic fibroblasts (wild type or AMPK ^-/-) were treated with 10 μM Nutlin 3a for 72 hours and examined by transmission electron microscopy for ‘mitophagic’ vacuoles.

<p>Mouse embryonic fibroblasts (wild type or AMPK ^-/-) were treated with 10 μM Nutlin 3a for 72 hours and examined by transmission electron microscopy for ‘mitophagic’ vacuoles.</p

A comprehensive glossary of autophagy-related molecules and processes (2nd edition)

The study of autophagy is rapidly expanding, and our knowledge of the molecular mechanism and its connections to a wide range of physiological processes has increased substantially in the past decade. The vocabulary associated with autophagy has grown concomitantly. In fact, it is difficult for readers—even those who work in the field—to keep up with the ever-expanding terminology associated with the various autophagy-related processes. Accordingly, we have developed a comprehensive glossary of autophagy-related terms that is meant to provide a quick reference for researchers who need a brief reminder of the regulatory effects of transcription factors and chemical agents that induce or inhibit autophagy, the function of the autophagy-related proteins, and the roles of accessory components and structures that are associated with autophagy