Additional file 2: Table S2. of High prevalence of Enterocytozoon bieneusi zoonotic genotype D in captive golden snub-nosed monkey (Rhinopithecus roxellanae) in zoos in China

Nucleotide substitutions among all sequences found in this study versus the reported genotype J (KU557671). (XLS 49 kb

Specific locations at which specimens were collected in this study.

<p>★locations of zoos.</p

Neighbor-joining tree of <i>E</i>. <i>bieneusi</i> ITS genotypes.

<p>Phylogenetic relationship of <i>E</i>. <i>bieneusi</i> ITS nucleotide sequences of this study and other genotypes previously deposited in GenBank, as inferred by a neighbor-joining analysis (software Mega 5, <a href="http://www.megasoftware.net/" target="_blank">http://www.megasoftware.net/</a>) based on genetic distances calculated using the Kimura two-parameter model. The ITS tree was rooted with GenBank sequence DQ885585. Bootstrap values greater than 50% from 1,000 are shown on nodes. Each sequence from GenBank is identified by the accession number, host origin, and the genotype designation. The group terminology for the clusters is based on the works of Thellier and Breton [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117991#pone.0117991.ref021" target="_blank">21</a>], Li et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117991#pone.0117991.ref009" target="_blank">9</a>], and Karim et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117991#pone.0117991.ref003" target="_blank">3</a>]. Two unique sequences of new genotype CM18 and known genotype CM4 in this study are designated as group 9 sequences. Known and new genotypes identified in this study are indicated by open and filled triangles, respectively.</p

Additional file 3 of Neospora caninum inhibits tumor development by activating the immune response and destroying tumor cells in a B16F10 melanoma model

Additional file 3: Figure S2. Visualization of N. caninum-GFP tachyzoites in B16F10 cells

Additional file 2 of Neospora caninum inhibits tumor development by activating the immune response and destroying tumor cells in a B16F10 melanoma model

Additional file 2: Figure S1. Evolution of body weight in tumor-bearing mice (n = 5) treated with PBS or N. caninum tachyzoites

Additional file 1 of Neospora caninum inhibits tumor development by activating the immune response and destroying tumor cells in a B16F10 melanoma model

Additional file 1: Table S1. The primers used for RT-PCR