Additional file 2: Table S2. of Host genetic diversity influences the severity of Pseudomonas aeruginosa pneumonia in the Collaborative Cross mice

Change in body weight of each CC line after P. aeruginosa airway. (DOCX 51 kb

Additional file 1: Table S1. of Host genetic diversity influences the severity of Pseudomonas aeruginosa pneumonia in the Collaborative Cross mice

Survival Time of each CC lines after P. aeruginosa airway infection. (DOCX 51 kb

Additional file 3: Table S3. of Host genetic diversity influences the severity of Pseudomonas aeruginosa pneumonia in the Collaborative Cross mice

Bonferroni’s Multiple Comparison Tests of recorded traits (MST and CBW1) among CC lines after P. aeruginosa airway infection. (DOCX 74 kb

Boletín de Segovia: Número 18 - 1895 febrero 11

Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200

Additional file 1: Figure S1. of Collaborative cross mice in a genetic association study reveal new candidate genes for bone microarchitecture

Phenotypic diversity between the CC lines. (A) phenotypic distribution among males and (B) females. From top-left, counter-clockwise: trabecular bone volume fraction (BV/TV; %), trabecular number (Tb.N; mm-1), thickness (Tb.Th; mm), and connectivity density (Conn.D; mm-3). (DOC 400 kb

Additional file 3: of Mapping genetic determinants of host susceptibility to Pseudomonas aeruginosa lung infection in mice

Effect of the Pairl1 locus on survival in A/J, C3H/HeOuJ and heterozygotes. (DOCX 106 kb

Additional file 5: of Mapping genetic determinants of host susceptibility to Pseudomonas aeruginosa lung infection in mice

List of the most promising candidates genes for susceptibility to acute P. aeruginosa pulmonary infection. (DOCX 27 kb

Transcription factors regulating erythropoiesis.

<p><i>Tal1</i>, <i>Gata1</i>, <i>Lmo2</i>, <i>Ldb1</i>, <i>TcfE2a</i> and <i>Zfpm1</i> (<i>Fog1</i>) form a multimeric DNA binding complex, which regulates primitive haematopoiesis. All six genes were highly expressed and had similar patterns of expression consistent with co-ordinate regulation. <i>Klf1</i> is involved in erythroid cell proliferation and had similar levels and patterns of expression suggesting that it may be regulated by the same mechanisms. In all cases C57BL/6 mice tended to have the lowest levels of expression after day 3.</p

Erythrocyte degradation and leukocyte abundance.

<p><i>Blvra</i> and <i>Hmox1</i>, which are involved in erythrocyte degradation, increased dramatically after infection but then declined to near baseline by day 17. <i>Cd14</i> and Cd45 (<i>Ptprc</i>) are markers of macrophage and leukocyte abundance respectively. Macrophages are the main cells involved in haemolysis and it appears that expression of <i>Blvra</i> and <i>Hmox1</i> was correlated with macrophage abundance. C57BL/6 did not have consistently higher expression of any of these genes, suggesting that higher or more chronic haemolysis is not the cause of the more chronic anaemia of this strain.</p

Expression of <i>Snca</i> in (A) liver and (B) spleen.

<p><i>Snca</i> is strongly associated with reticulocytes and was the gene with largest expression difference that correlated with anaemia response. The strong expression of <i>Snca</i> in the spleen of A/J and BALB/c is suggestive of extra medullary haematopoiesis in this organ in these strains.</p