Resveratrol enhances brown adipocyte formation and function by activating AMP-activated protein kinase (AMPK) α1 in mice fed high-fat diet

© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Scope: Enhancing the formation and function of brown adipose tissue (BAT) increases thermogenesis and hence reduces obesity. Thus, we investigate the effects of resveratrol (Resv) on brown adipocyte formation and function in mouse interscapular BAT (iBAT). Methods and results: CD1 mice and stromal vascular cells (SVCs) isolated from iBAT were treated with Resv. Expression of brown adipogenic and thermogenic markers, and involvement of AMP-activated protein kinase (AMPK)α1 were assessed. In vivo, Resv-enhanced expression of brown adipogenic markers, PR domain-containing 16 (PRDM16) and thermogenic genes, uncoupling protein 1 (UCP1) and cytochrome C in iBAT, along with smaller lipid droplets, elevated AMPKα activity and increased oxygen consumption. Meanwhile, Resv promoted expression of PRDM16, UCP1, PGC1α, cytochrome C and pyruvate dehydrogenase (PDH) in differentiated iBAT SVCs, suggesting that Resv enhanced brown adipocyte formation and function in vitro. In addition, Resv stimulated AMPKα and oxygen consumption in differentiated iBAT SVCs. However, the promotional effects of Resv were diminished by AMPK inhibition or AMPKα1 knockout, implying the involvement of AMPKα1 in this process. Conclusion: Resv enhanced brown adipocyte formation and thermogenic function in mouse iBAT by promoting the expression of brown adipogenic markers via activating AMPKα1, which contributed to the anti-obesity effects of Resv

A Satellite-Driven Model to Estimate Long-Term Particulate Sulfate Levels and attributable Mortality Burden in China

Ambient fine particulate matter (P

Ultra-Sensitive, Deformable and Transparent Triboelectric Tactile Sensor based on Micro-Pyramid Patterned Ionic Hydrogel for Interactive Human-Machine Interfaces

Rapid advances in wearable electronics and mechno-sensational human-machine interfaces impose great challenges in developing flexible and deformable tactile sensors with high efficiency, ultra-sensitivity, environment-tolerance and self-sustainability. Herein, we report a tactile hydrogel sensor (THS) based on micro-pyramid-patterned double-network (DN) ionic organohydrogels to detect subtle pressure changes by measuring the variations of triboelectric output signal without an external power supply. By the first time of pyramidal-patterned hydrogel fabrication method and laminated PDMS encapsulation process, the self-powered THS shows the advantages of remarkable flexibility, good transparency (~85), and excellent sensing performance, including extraordinary sensitivity (45.97 mV Pa-1 ), fast response (~20 ms), very low limit of detection (50 Pa) as well as high stability (36000 cycles). Moreover, with the LiBr immersion treatment method, the THS possesses excellent long-term hyper antifreezing and anti-dehydrating properties, broad environment tolerance (-20 to 60 ℃), and instantaneous peak power density of 20 μW cm-2 , providing reliable contact outputs with different materials and detecting very slight human motions. The THS shows no apparent output decline under the extreme environments of −29℃, 60℃ and even the vacuum conditions, demonstrating the excellent application potential in the field of harsh environments. By integrating the signal acquisition/process circuit, the THS with excellent self-power sensing ability is utilized as a switching button to control electric appliances and robotic hands by simulating human finger gestures, offering its great potentials for wearable and multi-functional electronic applications