Zertifikatskurs der Universität Freiburg: Coaching und Mentoring in der Lehrerbildung

How to develop teaching competencies that meet research-based standards of effective teaching is the guiding question at the core of initial teacher education, teacher induction and in-service professional development. There is a growing consensus that such competencies can be advanced significantly in practice-based settings in which (future) teachers are assisted through qualified coaching and/or mentoring. Participants of University of Fribourg’s certified course on coaching and mentoring in teacher education, as described in this paper, study exemplary models of coaching and mentoring and develop at the individual as well as at the institutional level new ways of assisting teacher learning in initial practice-based teacher education, teacher induction and in-service professional development.Für die Grundausbildung und Weiterbildung von Lehrpersonen stellt sich die zentrale Frage, wie professionelle Handlungskompetenzen aufgebaut werden können, die sich an empirisch bewährten Merkmalen lernwirksamen Unterrichts orientieren. Zunehmend setzt sich die Überzeugung durch, dass hierzu das durch qualifizierte Formen von Coaching und/oder Mentoring unterstützte Lernen in der Praxis eine wichtige Rolle spielen kann. Die Teilnehmerinnen und Teilnehmer des in diesem Artikel beschriebenen Zertifikatskurses Coaching und Mentoring in der Lehrerbildung der Universität Freiburg setzen sich mit unterschiedlichen Ansätzen von Coaching und Mentoring auseinander. Sie erhalten dabei Anregungen und Hilfestellungen für die berufspraktische Ausbildung, die Berufseinführung und Weiterbildung von Lehrpersonen, die sie sowohl für ihr eigenes Handeln wie auch für Entwicklungen innerhalb ihrer Institutionen nutzen können

In vivo imaging and quantitative analysis of leukocyte directional migration and polarization in inflamed tissue

Directional migration of transmigrated leukocytes to the site of injury is a central event in the inflammatory response. Here, we present an in vivo chemotaxis assay enabling the visualization and quantitative analysis of subtype-specific directional motility and polarization of leukocytes in their natural 3D microenvironment. Our technique comprises the combination of i) semi-automated in situ microinjection of chemoattractants or bacteria as local chemotactic stimulus, ii) in vivo near-infrared reflected-light oblique transillumination (RLOT) microscopy for the visualization of leukocyte motility and morphology, and iii) in vivo fluorescence microscopy for the visualization of different leukocyte subpopulations or fluorescence-labeled bacteria. Leukocyte motility parameters are quantified off-line in digitized video sequences using computer-assisted single cell tracking. Here, we show that perivenular microinjection of chemoattractants [macrophage inflammatory protein-1alpha (MIP-1alpha/Ccl3), platelet-activating factor (PAF)] or E. coli into the murine cremaster muscle induces target-oriented intravascular adhesion and transmigration as well as polarization and directional interstitial migration of leukocytes towards the locally administered stimuli. Moreover, we describe a crucial role of Rho kinase for the regulation of directional motility and polarization of transmigrated leukocytes in vivo. Finally, combining in vivo RLOT and fluorescence microscopy in Cx3CR1(gfp/gfp) mice (mice exhibiting green fluorescent protein-labeled monocytes), we are able to demonstrate differences in the migratory behavior of monocytes and neutrophils.Taken together, we propose a novel approach for investigating the mechanisms and spatiotemporal dynamics of subtype-specific motility and polarization of leukocytes during their directional interstitial migration in vivo