Appunti di informatica per le biblioteche

This text is an overview about library automation. It starts with information retrieval, metadata, MARC, XML and other useful definition of standards and formats. The authors then approach tools such as ILS, OPAC and discovery tools, and sources (full text, such as digital libraries and e-journals, and bibliographic ones, such as citation databases). The last part is a brief history of library automation in Italy

Appunti di informatica per le biblioteche

This text is an overview about library automation. It starts with information retrieval, metadata, MARC, XML and other useful definition of standards and formats. The authors then approach tools such as ILS, OPAC and discovery tools, and sources (full text, such as digital libraries and e-journals, and bibliographic ones, such as citation databases). The last part is a brief history of library automation in Italy

First record and establishment of Tuberocephalus (Trichosiphoniella) tianmushanensis Zang, (Hemiptera Aphididae) on ornamental cherry trees in Italy

The occurrence of the Asiatic aphid Tuberocephalus (Trichosiphoniella) tianmushanensis Zang, (=Tuberocephalus (Trichosiphoniella) higansakurae hainnevilleae Remaudière and Sorin) (Hemiptera Aphididae) in Italy is reported. The species was first detected inside leaf galls of Prunus subhirtella cv. pendula trees growing outdoors at the University Botanical Garden of Padua (Italy). Further investigations demonstrated that the species is present in plant nurseries in the Veneto region. So far this species was considered eradicated in Europe, after its first incursion in France in 1993

The Human Cytomegalovirus UL116 Glycoprotein Is a Chaperone to Control gH-Based Complexes Levels on Virions

Human cytomegalovirus (HCMV) relies in large part upon the viral membrane fusion glycoprotein B and two alternative gH/gL complexes, gH/gL/gO (Trimer) and gH/gL/UL128/UL130/UL131A (Pentamer) to enter into cells. The relative amounts of Trimer and Pentamer vary among HCMV strains and contribute to differences in cell tropism. Although the viral ER resident protein UL148 has been shown to interact with gH to facilitate gO incorporation, the mechanisms that favor the assembly and maturation of one complex over another remain poorly understood. HCMV virions also contain an alternative non-disulfide bound heterodimer comprised of gH and UL116 whose function remains unknown. Here, we show that disruption of HCMV gene UL116 causes infectivity defects of ∼10-fold relative to wild-type virus and leads to reduced expression of both gH/gL complexes in virions. Furthermore, gH that is not covalently bound to other viral glycoproteins, which are readily detected in wild-type HCMV virions, become undetectable in the absence of UL116 suggesting that the gH/UL116 complex is abundant in virions. We find evidence that UL116 and UL148 interact during infection indicating that the two proteins might cooperate to regulate the abundance of HCMV gH complexes. Altogether, these results are consistent with a role of UL116 as a chaperone for gH during the assembly and maturation of gH complexes in infected cells

Recombinant outer membrane vesicles carrying Chlamydia muridarum HtrA induce antibodies that neutralize chlamydial infection in vitro

Background: Outer membrane vesicles (OMVs) are spheroid particles released by all Gram-negative bacteria as a result of the budding out of the outer membrane. Since they carry many of the bacterial surface-associated proteins and feature a potent built-in adjuvanticity, OMVs are being utilized as vaccines, some of which commercially available. Recently, methods for manipulating the protein content of OMVs have been proposed, thus making OMVs a promising platform for recombinant, multivalent vaccines development. Methods: Chlamydia muridarum DO serine protease HtrA, an antigen which stimulates strong humoral and cellular responses in mice and humans, was expressed in Escherichia coli fused to the OmpA leader sequence to deliver it to the OMV compartment. Purified OMVs carrying HtrA (CM rHtrA-OMV) were analyzed for their capacity to induce antibodies capable of neutralizing Chlamydia infection of LLC-MK2 cells in vitro. Results: CM rHtrA-OMV immunization in mice induced antibodies that neutralize Chlamydial invasion as judged by an in vitro infectivity assay. This was remarkably different from what observed with an enzymatically functional recombinant HtrA expressed in, and purified from the E. coli cytoplasm (CM rHtrA). The difference in functionality between anti-CM rHtrA and anti-CM rHtrA-OMV antibodies was associated to a different pattern of protein epitopes recognition. The epitope recognition profile of anti-CM HtrA-OMV antibodies was similar to that induced in mice during Chlamydial infection. Conclusions: When expressed in OMVs HtrA appears to assume a conformation similar to the native one and this results in the elicitation of functional immune responses. These data further support the potentiality of OMVs as vaccine platform

Libraries in Italy : a brief overview

The Italian library network is complex and difficult to explain. This article reports some characteristics of this system, that comprises State, university, special, public, school and ecclesiastical libraries, foundations and privately owned structures. An overview of the main historical features is given, as well as present-day major trends, such as education, legislation, new library projects, etc. Concludes with proposals for new national services

La diffusione di software Open Source per la gestione di biblioteche in Italia

Outcomes of a research made at the beginning on 2009 about the presence of open source integrated systems in Italian librarie

Linee guida per la visualizzazione degli OPAC (On line Public Access Catalogue). Edizione italiana degli esempi

Sample screenshot of OPACs published with Guidelines for Online Public Access Catalogue (OPAC) Displays. The Guidelines are published in OPAC / Andrea Marchitelli, Giovanna Frigimelica. Roma: AIB, 201