Full field electroretinogram in autism spectrum disorder

Purpose To explore early findings that individuals with autism spectrum disorder (ASD) have reduced scotopic ERG b-wave amplitudes. Methods Dark adapted (DA) ERGs were acquired to a range of flash strengths, (-4.0 to 2.3 log phot cd.s.m-2), including and extending the ISCEV standard, from two subject groups: (ASD) N=11 and (Control) N=15 for DA and N=14 for light adapted (LA) ERGs who were matched for mean age and range. Naka-Rushton curves were fitted to DA b-wave amplitude growth over the first limb (-4.0 to -1.0 log phot cd.s.m-2). The derived parameters (Vmax, Km and n) were compared between groups. Scotopic 15 Hz flicker ERGs (14.93Hz) were recorded to 10 flash strengths presented in ascending order from -3.0 to 0.5 log Td.s to assess the slow and fast rod pathways respectively. LA ERGs were acquired to a range of flash strengths, (-0.5 to 1.0 log phot cd.s.m-2). Photopic 30 Hz, flicker ERGs, oscillatory potentials (OPs) and the responses to prolonged 120 ms ON- OFF stimuli were also recorded. Results For some individuals the DA b-wave amplitudes fell below the control 5th centile of the controls with up to four ASD participants (36%) at the 1.5 log phot cd.s.m-2 flash strength and two (18%) ASD participants at the lower -2 log phot cd.s.m-2 flash strength. However, across the thirteen flash strengths there were no significant group differences for b-wave amplitude’s growth (repeated measures ANOVA p=0.83). Nor were there any significant differences between the groups for the Naka-Rushton parameters (p>0.09). No group differences were observed in the 15Hz scotopic flicker phase or amplitude (p>0.1), DA ERG a- wave amplitude or time to peak (p>26). The DA b-wave time to peak at 0.5 log phot cd.s.m-2 were longer in the ASD group (corrected p=0.04). The single ISCEV LA 0.5 log phot cd.s.m-2 (p0.08) to the single flash stimuli although there was a significant interaction between group and flash strength for the b-wave amplitude (corrected p=0.006). The prolonged 120 ms ON-responses were smaller in the ASD group (corrected p=0.003), but the OFF response amplitude (p>0.6) and ON and OFF times to peaks (p>0.4) were similar between groups. The LA OPs showed an earlier bifurcation of OP2 in the younger ASD participants, however no other differences were apparent in the OPs or 30Hz flicker waveforms. Conclusion Some ASD individuals show subnormal DA ERG b-wave amplitudes. Under LA conditions the b-wave is reduced across the ASD group along with the ON response of the ERG. These exploratory findings, suggest there is altered cone-ON bipolar signalling in ASD

Income-Tested College Financial Aid and Labor Disincentives

Working has become commonplace among college students; however, this activity can have unexpected financial consequences. Federal formulas implicitly tax the amount of financial aid students are eligible to receive by as much as 50 cents for each marginal dollar of income. This tax creates an incentive for college students to reduce income, though abstruse formulas and the timing of financial aid receipt are likely to limit responses. Using data from a national sample of financially independent college students in the United States, I do not find that students bunch below earnings protection thresholds in a manner that would indicate attempts to avoid reductions in financial aid in total or grants specifically. Moreover, I do not find evidence that implicit income taxes predict lower earnings in a manner that suggests that students meaningfully reduce earnings in response to the tax. Therefore, while economically efficient, the reduction in aid has the potential to burden low-income students who need to both work and receive financial aid in order to afford college expenses

Time course of the flash response of dark- and light-adapted human rod photoreceptors derived from the electroretinogram

The a-wave of the electroretinogram was recorded from human subjects with normal vision, using a corneal electrode and ganzfeld stimulation. We applied the paired-flash technique, in which an intense ‘probe’ flash was delivered at different times after a ‘test’ flash. The amplitude of the probe-flash response provided a measure of the circulating current remaining at the appropriate time after the test flash.We extended previous methods by measuring not at a fixed time, but at a range of times after the probe flash, and then calculating the ratio of the ‘test-plus-probe’ response to the ‘probe-alone’ response, as a function of time.Under dark-adapted conditions the rod response derived by the paired-flash technique (in response to a relatively dim test flash) peaked at ca 120 ms, with a fractional sensitivity at the peak of ca 0.1 Td−1 s−1.As reported previously, background illumination reduced the maximal response, reflecting a reduction in rod circulating current. In addition, it shortened the time to peak (to ca 70 ms at an intensity of 170 Td), and reduced the flash sensitivity measured at the peak. The flash sensitivity declined approximately according to Weber's Law, with a 10-fold reduction occurring at an intensity of 100-200 Td. We could not reliably measure responses at significantly higher background intensities because the circulating current became so small.In order to investigate the phototransduction process after correction for response compression, we expressed the derived response as a fraction of the maximal response that could be elicited in the presence of the background. The earliest rising phase of this ‘fractional response per unit intensity’ was little affected by background illumination, suggesting that the amplification constant of transduction was unaltered by light adaptation

Human cone photoreceptor responses measured by the electroretinogram a-wave during and after exposure to intense illumination

We recorded the a-wave of the electroretinogram from human subjects with normal vision, using a corneal fibre electrode and ganzfeld stimulation under photopic conditions, so as to extract the parameters of cone phototransduction. The amplitude of bright flash responses provided a measure of the massed circulating current of the cones, while the amplitude of dim flash responses provided a measure of the product of the fraction of cone photopigment present, and the amplification constant of transduction within the cones.In the presence of steady background illumination, the cone circulating current declined to half at 3000 photopic trolands, and to a quarter at 20 000 photopic trolands.At very early times after the delivery of a near-total bleach, we could not determine the level of circulating current as our bright flashes did not appear to saturate the a-wave (presumably because so little pigment was present). However, by 20–30 s after a total bleach, the cone circulating current had returned to its dark-adapted level.Following smaller bleaches (when ca 50 % of the pigment remained present) the bright flashes were able to saturate the a-wave even at very early times. Within 3 s of extinction of the illumination, the cone circulating current had returned to its dark-adapted level.This is at least a factor of 300 times faster than the period of ca 15 min required for full recovery of rods exposed to the same level of bleach, and indicates a major difference between rods and cones in the way that they cope with the photoproducts of bleaching.Despite the very rapid recovery of circulating current after bleaches, the recovery of dim-flash sensitivity was much slower, with a time constant of ca 1.5 min after a near-total bleach. This time course is very similar to previous measurements of the regeneration of cone photopigment, and it seems highly probable that the reduction in dim-flash sensitivity results from pigment depletion