Profile of the Spleen Transcriptome in Beef Steers with Variation in Gain and Feed Intake

We have previously identified components of the immune system contributing to feed intake and gain in both the rumen and small intestine of beef steers. In this study, we examined the spleen, a major lymphatic organ near the digestive tract, to determine whether it was also influencing individual feed efficiency status through immune responses. Animals (n=16) that were divergent for gain and intake were selected for tissue sampling. The spleen transcriptomes were evaluated by microarray. A total of 1,216 genes were identified as differentially expressed. Genes were over-represented in Kyoto encyclopedia of genes and genomes (KEGG) pathways including biological regulation, protein folding, cell communication, immune systems process, response to stress and RNA metabolic process. Several stress response or heat shock genes including HSPH1, HSPA1A, HSPA4, DNAJB4, DNAJA4, etc., were identified as a stress response functional gene cluster in the low gain-low intake animals. These genes were up-regulated amongst the low gain-low intake animals compared to all other groups. Canonical pathways associated with the differentially expressed genes included the coagulation system, extrinsic prothrombin activation, protein ubiquitination, unfolded protein response and aldosterone signaling in epithelial cells. An analysis of expressed copy number variable (CNV) genes in the spleen produced some of the same genes and gene families that were differentially expressed. Our data suggests the splenic contribution to some of the underlying variation among gain and intake within this group of animals may be a result of immune function and stress response. In addition, some of the differences in immune response functions may be related to gene copy number

Caffeine and Anxiety: Is Caffeine an Underlying Source of Anxiety in College Students?

Both anxiety and high consumptions of caffeine are experienced at high rates among the college student population. This study examined how the continued presence or current removal of caffeine affects anxiety levels using 9 psychology students recruited from Winona State University. Participants were randomly assigned to either the control or experimental condition. On Day 1 and Day 5 of their participation, participant’s state and trait anxiety were measured using the STAI-AD test. An independent samples t-test showed there was a significant difference between groups which supported they hypothesis that those who cease caffeine use for five days would experience decreased anxiety symptoms compared to those that did not. These findings indicate that female college students are using caffeine at levels that are contributing significantly to their anxiety. These data suggest that wellness programs on college campuses should include discussions to reduce caffeine consumption in college women

Feed Efficiency And The Microbiota Of The Alimentary Tract

There is considerable variation in the efficiency that cattle convert feed for maintenance and product (body weight gain, milk, and conceptus). Both intake and gain are polygenic traits and to better understand factors that contribute to variation in feed efficiency more defined phenotypes are needed. Several studies have associated differences in the microbiota of the alimentary tract between obese and non-obese rodents (Turnbaugh et al., 2006), obese and nonobese humans (Ley et al., 2006), and energy metabolism in birds (Torok et al., 2008 and Stanley et al., 2013). These finding suggest that there is a potential relationship between the microbiota of the alimentary tract and feed efficiency in beef cattle. Considerable research has been conducted on the rumen microbiota, but less consideration has been given to the rest of the alimentary tract

Feed Efficiency And The Microbiota Of The Alimentary Tract

There is considerable variation in the efficiency that cattle convert feed for maintenance and product (body weight gain, milk, and conceptus). Both intake and gain are polygenic traits and to better understand factors that contribute to variation in feed efficiency more defined phenotypes are needed. Several studies have associated differences in the microbiota of the alimentary tract between obese and non-obese rodents (Turnbaugh et al., 2006), obese and nonobese humans (Ley et al., 2006), and energy metabolism in birds (Torok et al., 2008 and Stanley et al., 2013). These finding suggest that there is a potential relationship between the microbiota of the alimentary tract and feed efficiency in beef cattle. Considerable research has been conducted on the rumen microbiota, but less consideration has been given to the rest of the alimentary tract

Growth curves of crossbred cows sired by Hereford, Angus, Belgian Blue, Brahman, Boran, and Tuli bulls, and the fraction of mature body weight and height at puberty

The objective of this study was to evaluate the growth curves of females to determine if mature size and relative rates of maturation among breeds differed. Body weight and hip height data were fitted to the nonlinear function BW = f(age) = A − Bek×age, where A is an estimate of mature BW and k determines the rate that BW or height moves from B to A. Cows represented progeny from 28 Hereford, 38 Angus, 25 Belgian Blue, 34 Brahman, 8 Boran, and 9 Tuli sires. Bulls from these breeds were mated by AI to Angus, Hereford, and U.S. Meat Animal Research Center III composite (1/4 Angus, ¼ Hereford, 1/4 Red Poll, and 1/4 Pinzgauer) cows to produce calves in 1992, 1993, and 1994. These matings resulted in 516 mature cows whose growth curves were subsequently evaluated. Hereford-sired cows tended to have heavier mature BW, as estimated by parameter A, than Angus- (P = 0.09) and Brahman-sired cows(P = 0.06), and were heavier than the other breeds (P \u3c 0.001). Angus-sired cows were heavier than Boran-(P \u3c 0.001) and Tuli-sired cows (P \u3c 0.001), and tended to be heavier than Belgian Blue-sired cows (P = 0.097). Angus-sired cows did not differ from Brahman- sired cows (P = 0.94). Brahman-sired cows had a heavier mature BW than Boran- (P \u3c 0.001), Tuli- (P \u3c 0.001), and Belgian Blue-sired cows (P \u3c 0.04). Angus-sired cows matured faster (k) than cows sired by Hereford (P = 0.03), Brahman (P \u3c 0.001), Boran (P = 0.03), and Tuli (P \u3c 0.001) sires, but did not differ from Belgian Blue-sired (P = 0.13) cows. Brahmansired cows took longer to mature than Boran- (P = 0.03) or Belgian Blue-sired cows (P = 0.003). Belgian Blue-sired cows were faster maturing than Tuli-sired cows (P = 0.02). Brahman-sired cows had reached a greater proportion of their mature BW at puberty than had Hereford- (P \u3c 0.001), Tuli- (P = 0.003), and Belgian Blue-sired cows (P = 0.001). Boran-sired cows tended to have reached a greater proportion of their mature BW at puberty than had Angus-sired cows (P = 0.09), and had reached a greater proportion of their mature BW at puberty than had Hereford- (P \u3c 0.001), Tuli- (P \u3c 0.001), and Belgian Blue-sired cows (P \u3c 0.001). Within species of cattle, the relative range in proportion of mature BW at puberty (Bos taurus 0.56 through 0.58, and Bos indicus 0.60) was highly conserved, suggesting that proportion of mature BW is a more robust predictor of age at puberty across breeds than is absolute weight or age

Growth curves of crossbred cows sired by Hereford, Angus, Belgian Blue, Brahman, Boran, and Tuli bulls, and the fraction of mature body weight and height at puberty

The objective of this study was to evaluate the growth curves of females to determine if mature size and relative rates of maturation among breeds differed. Body weight and hip height data were fitted to the nonlinear function BW = f(age) = A − Bek×age, where A is an estimate of mature BW and k determines the rate that BW or height moves from B to A. Cows represented progeny from 28 Hereford, 38 Angus, 25 Belgian Blue, 34 Brahman, 8 Boran, and 9 Tuli sires. Bulls from these breeds were mated by AI to Angus, Hereford, and U.S. Meat Animal Research Center III composite (1/4 Angus, ¼ Hereford, 1/4 Red Poll, and 1/4 Pinzgauer) cows to produce calves in 1992, 1993, and 1994. These matings resulted in 516 mature cows whose growth curves were subsequently evaluated. Hereford-sired cows tended to have heavier mature BW, as estimated by parameter A, than Angus- (P = 0.09) and Brahman-sired cows(P = 0.06), and were heavier than the other breeds (P \u3c 0.001). Angus-sired cows were heavier than Boran-(P \u3c 0.001) and Tuli-sired cows (P \u3c 0.001), and tended to be heavier than Belgian Blue-sired cows (P = 0.097). Angus-sired cows did not differ from Brahman- sired cows (P = 0.94). Brahman-sired cows had a heavier mature BW than Boran- (P \u3c 0.001), Tuli- (P \u3c 0.001), and Belgian Blue-sired cows (P \u3c 0.04). Angus-sired cows matured faster (k) than cows sired by Hereford (P = 0.03), Brahman (P \u3c 0.001), Boran (P = 0.03), and Tuli (P \u3c 0.001) sires, but did not differ from Belgian Blue-sired (P = 0.13) cows. Brahmansired cows took longer to mature than Boran- (P = 0.03) or Belgian Blue-sired cows (P = 0.003). Belgian Blue-sired cows were faster maturing than Tuli-sired cows (P = 0.02). Brahman-sired cows had reached a greater proportion of their mature BW at puberty than had Hereford- (P \u3c 0.001), Tuli- (P = 0.003), and Belgian Blue-sired cows (P = 0.001). Boran-sired cows tended to have reached a greater proportion of their mature BW at puberty than had Angus-sired cows (P = 0.09), and had reached a greater proportion of their mature BW at puberty than had Hereford- (P \u3c 0.001), Tuli- (P \u3c 0.001), and Belgian Blue-sired cows (P \u3c 0.001). Within species of cattle, the relative range in proportion of mature BW at puberty (Bos taurus 0.56 through 0.58, and Bos indicus 0.60) was highly conserved, suggesting that proportion of mature BW is a more robust predictor of age at puberty across breeds than is absolute weight or age

Psychological Factors that Impact White Counseling Trainees’ Responses to Cultural Ruptures

In the field of counseling and clinical psychology, the last several decades have been characterized by a strengthened recognition of the importance of cultural factors in psychotherapy. While this has been impactful, there is evidence that cultural ruptures, microaggressions, and racial/ethnic disparities in psychotherapy outcomes persist. Aversive racism theory, which provides explanations for the racist tendencies typically associated with progressive White individuals, postulates that a conflict between explicit egalitarian beliefs and implicit negative racial biases impedes White individuals from adequately addressing and acknowledging underlying biases. Therefore, it was hypothesized that psychological factors, such as defense mechanisms, professional selfdoubt and self-compassion, may play a role in impeding or enhancing therapists’ ability to identify and resolve cultural ruptures in therapy. The present study sought to test these hypotheses by asking White therapists-in-training to respond to video vignettes portraying cultural ruptures. These vignettes were then coded to assess the level of cultural comfort, cultural humility and cultural opportunities demonstrated by participants. Results revealed that self-compassion was positively associated with White trainees’ cultural humility and overall effectiveness, specifically in the vignette that included a client-confrontation response. Implications, limitations and future directions are discussed

Comparison of Semi-confined and Pasture-based August Calving Beef Cow Systems

Limited pasture availability and increased pasture rental rates have generated a need to evaluate alternative cow-calf production systems. The current study compared cow and calf performance in two August-calving cow systems that combined corn residue grazing with 1) perennial forage grazing and hay or 2) summer drylot feeding and fall cover crop grazing. Differences in pregnancy rates between systems within year were not observed; however, the effect of production system on cow body condition and calf body weight at different time points varied across years. Overall, cow and calf performance were not negatively impacted in the drylot/cropland system, suggesting that it is a potential alternative to a perennial forage-based system

Effects of decreased dietary roughage concentration on energy metabolism and nutrient balance in finishing beef cattle

The optimal roughage concentration required in feedlot diets changes continuously for many reasons such as source, availability, price, and interaction with other ingredients in the diet. Wet distillers grains and solubles (WDGS) are common in finishing diets and they contain relatively high amounts of fiber compared with other grains they replace. Therefore, concentration of roughage could be altered when WDGS are included in feedlot diets. There has been very little data published regarding the effects of roughage concentration on energy metabolism and nutrient balance in beef steers. Therefore, the effects of roughage concentration in dryrolled corn (DRC)–based diets containing 25% WDGS were evaluated in 8 steers (BW = 362 ± 3.71 kg) using a replicated Latin square. Data were analyzed with the fixed effects of dietary treatment and period and random effects of square and steer within square were included in the model. Diets consisted of 25% WDGS and the balance being DRC and coarsely ground alfalfa hay (AH) replacing corn at 2% (AH-2), 6% (AH-6), 10% (AH-10), and 14% (AH-14) of dietary dry matter. As a proportion of GE intake, fecal energy loss increased linearly (P = 0.02), and DE decreased linearly (P = 0.02) as dietary level of AH increased. Methane energy loss, as a proportion of GE intake, increased linearly (P \u3c 0.01) and ME decreased linearly (P \u3c 0.01) as dietary concentration of AH increased. Heat production tended (P = 0.10) to decrease reaching a minimum of 10% AH and increased from 10 to 14% AH inclusion. Moreover, as a proportion of GE intake, retained energy (RE) decreased (P \u3c 0.01) as AH level increased in the diet. Reasons for the decrease in RE are 1) the increase in fecal energy loss that is associated with decreased ruminal digestibility of NDF when AH replaced DRC and the shift in ruminal VFA produced, 2) the decreased energy available for animal retention when NDF increased linearly as AH increased in the diet, and 3) the methane and heat energy associated with digestion of the fibrous portion of the AH. Neutral detergent fiber and OM excretion also increased linearly (P \u3c 0.01) with increasing AH in the diet. The increased NDF and OM excretion were likely caused by the difference in digestibility of AH and DRC

Effects of decreased dietary roughage concentration on energy metabolism and nutrient balance in finishing beef cattle

The optimal roughage concentration required in feedlot diets changes continuously for many reasons such as source, availability, price, and interaction with other ingredients in the diet. Wet distillers grains and solubles (WDGS) are common in finishing diets and they contain relatively high amounts of fiber compared with other grains they replace. Therefore, concentration of roughage could be altered when WDGS are included in feedlot diets. There has been very little data published regarding the effects of roughage concentration on energy metabolism and nutrient balance in beef steers. Therefore, the effects of roughage concentration in dryrolled corn (DRC)–based diets containing 25% WDGS were evaluated in 8 steers (BW = 362 ± 3.71 kg) using a replicated Latin square. Data were analyzed with the fixed effects of dietary treatment and period and random effects of square and steer within square were included in the model. Diets consisted of 25% WDGS and the balance being DRC and coarsely ground alfalfa hay (AH) replacing corn at 2% (AH-2), 6% (AH-6), 10% (AH-10), and 14% (AH-14) of dietary dry matter. As a proportion of GE intake, fecal energy loss increased linearly (P = 0.02), and DE decreased linearly (P = 0.02) as dietary level of AH increased. Methane energy loss, as a proportion of GE intake, increased linearly (P \u3c 0.01) and ME decreased linearly (P \u3c 0.01) as dietary concentration of AH increased. Heat production tended (P = 0.10) to decrease reaching a minimum of 10% AH and increased from 10 to 14% AH inclusion. Moreover, as a proportion of GE intake, retained energy (RE) decreased (P \u3c 0.01) as AH level increased in the diet. Reasons for the decrease in RE are 1) the increase in fecal energy loss that is associated with decreased ruminal digestibility of NDF when AH replaced DRC and the shift in ruminal VFA produced, 2) the decreased energy available for animal retention when NDF increased linearly as AH increased in the diet, and 3) the methane and heat energy associated with digestion of the fibrous portion of the AH. Neutral detergent fiber and OM excretion also increased linearly (P \u3c 0.01) with increasing AH in the diet. The increased NDF and OM excretion were likely caused by the difference in digestibility of AH and DRC