Calreticulin expression in <i>DIO2</i> overexpressing hBMSCs.

<p>qPCR expression of <i>Calr</i> in hBMSCs transduced with either a control virus-vector (eGFP) or a <i>DIO2</i>-eGFP vector. Values of the RT-qPCR are displayed as the average±SEM, normalized for <i>GAPDH</i> expression and relative to the control sample at every timepoint. Differences were analyzed with Student T-Test ((*) P < 0.05).</p

Overexpressing <i>Calr</i> resulted in significantly lower Alcian Blue (AB) staining intensities.

<p>Staining intensities of Alcian Blue staining measured with a photospectrometer (620 nm). Values are displayed as the average±SEM, relative to the control sample. Differences were analyzed with Student T-Test ((*) P < 0.05).</p

Calreticulin expression stratified for treadmill-running and knockout.

<p>Representation of the <i>Calr</i> expression values of all individual samples plotted by genotype-group (Wild-type and <i>Dio2</i>^<i>-/-</i>) and treadmill-running-group (No Run and Run). Showing the significant reduction of <i>Calr</i> expression upon forced treadmill-running in wild-type-mice (<i>P</i> = 0,005) and the absence of change in the knockout-mice. P-values depicted are derived from Student T-Test.</p

Expression analyses in <i>Calr</i> overexpressing ATDC5 cells.

<p>RT-qPCR expression of <i>Calr</i>, <i>Dio2 and Acan</i> in ATDC5 cells transfected with either a <i>Calr</i>-containing vector for overexpression (<i>Calr</i>+) or 3 unique 27mer siRNA duplexes, specific for Calr, for knockdown (<i>Calr</i>-). Values of the RT-qPCR are displayed as the average±SEM, normalized for <i>GAPDH</i> expression and relative to the control samples (dashed line). Differences were analyzed with Student T-Test ((*) P < 0.05).</p