26 research outputs found
Partial molecular characterization of Sm8, a tegumental antigen of Schistosoma mansoni
Sm8 is a major tegumental antigen of Schistosoma mansoni. The partial cDNA was isolated and analyzed. Sequence analysis revealed transmembrane compatible hydrophobic domains and a putative leucine zipper pattern. The mRNA and the protein are predominantly expressed in adult worms
Antibody isotype responses to egg antigens in human chronic Schistosomiasis mansoni before and after treatment
In the present communication we analyzed the levels of IgG1, IgG2, IgG3, IgG4 and IgE isotypes to soluble egg antigen of Schistosoma mansoni by ELISA in individuals from an endemic area for schistosomiasis in Northeast Brazil. The analysis was performed before and after treatment to evaluate the age-dependent pattern, and to identify differences in the reactivities to antigens. Our results suggest that schistosomiasis treatment would not interfere with this sort of immune response
Molecular approaches for the detection of Schistosoma mansoni : possible applications in the detection of snail infection, monitoring of transmission sites, and diagnosis of human infection
The detection of specific DNA sequences by polymerase chain reaction
(PCR) has proved extremely valuable for the analysis of genetic
disorders and the diagnosis of a variety of infectious disease
pathogens. However, the application to the detection of Schistosoma
mansoni is rare, despite a recommendation of the World Health
Organization that a major focus of research on schistosomiasis should
be on the development and evaluation of new strategies and tools for
control of the disease. In this context, a few studies were published
for the detection of the parasite in snails, monitoring of cercariae in
water bodies, and diagnosis of human infection. The present minireview
describes sensitive and specific PCR based systems to detect S.
mansoni, indicating possible applications in the detection of snail
infection, monitoring of transmission sites, and diagnosis of human
infection