Characteristics of the 31 selected genes.

<p>Characteristics of the 31 selected genes.</p

Large-scale studies assessing gene expression changes in human blood after particle irradiation.

<p>Large-scale studies assessing gene expression changes in human blood after particle irradiation.</p

Large-scale studies assessing gene expression changes in mouse blood after irradiation.

<p>Large-scale studies assessing gene expression changes in mouse blood after irradiation.</p

Flow diagram outlining the selection procedure to identify 24 articles that were included in the systematic review of gene radiation dosimetry biomarkers in human blood.

<p>Flow diagram outlining the selection procedure to identify 24 articles that were included in the systematic review of gene radiation dosimetry biomarkers in human blood.</p

AUC and diagnostic accuracies of the 33 genes discriminating radiation dose <2Gy and ≥2Gy classified according to their maximized Youden’s index.

<p>AUC and diagnostic accuracies of the 33 genes discriminating radiation dose <2Gy and ≥2Gy classified according to their maximized Youden’s index.</p

Estimated specificity, positive predictive value rate, false positive and Youden’s index of the 33 genes discriminating radiation dose <2Gy and ≥2Gy at fixed sensitivity of 100%.

<p>Estimated specificity, positive predictive value rate, false positive and Youden’s index of the 33 genes discriminating radiation dose <2Gy and ≥2Gy at fixed sensitivity of 100%.</p

Receiver operating characteristic (ROC) curve analysis of IER5 + TNFSF4 combination that displays area under the ROC curve (AUC) ≥ 0.99 to discriminate radiation dose < 2 Gy from radiation dose ≥ 2 Gy.

<p>Receiver operating characteristic (ROC) curve analysis of IER5 + TNFSF4 combination that displays area under the ROC curve (AUC) ≥ 0.99 to discriminate radiation dose < 2 Gy from radiation dose ≥ 2 Gy.</p

Optimization of an Antibody Microarray Printing Process Using a Designed Experiment

Antibody microarrays have proven useful in immunoassay-based point-of-care diagnostics for infectious diseases. Noncontact piezoelectric inkjet printing has advantages to print antibody microarrays on nitrocellulose substrates for this application due to its compatibility with sensitive solutions and substrates, simple droplet control, and potential for high-capacity printing. However, there remain real-world challenges in printing such microarrays, which motivated this study. The effects of three concentrations of capture antibody (cAb) reagents and nozzle hydrostatic pressures were chosen to investigate three responses: the number of printed membrane disks, dispensing performance, and microarray quality. Printing conditions were found to be most ideal with 5 mg/mL cAb and a nozzle hydrostatic pressure near zero, which produced 130 membrane disks in a single print versus the 10 membrane disks per print before optimization. These results serve to inform efficient printing of antibody microarrays on nitrocellulose membranes for rapid immunoassay-based detection of infectious diseases and beyond