The impact of different DNA extraction kits and laboratories upon the assessment of human gut microbiota composition by 16S rRNA gene sequencing

Peer reviewedPublisher PD

Comparison of DNA yields between extraction methods.

<p>Comparison of DNA yields between extraction methods.</p

Study protocol.

<p>IMS: Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen; RI: Rowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, Aberdeen.</p

Multiple linear modelling after correction for multiple testing shows OTUs with significantly different relative abundance after extraction with the MoBio kit.

<p>Samples were excluded if all data points for that bacterial family were <0.5%. Reference sample was from patient H3 using either FastDNA method and extracted at the Institute of Medical Sciences. Differences are shown as fold change with 95% confidence intervals.</p

Linear modelling of family-level pyrosequencing data.

<p>RINH: Rowett Institute of Nutrition and Health.</p><p>Participants were excluded if all data points for that bacterial family were < 0.5%. Reference sample was from participant H3 using FastDNA method 1 and extracted at the Institute of Medical Sciences. Differences are shown as fold change with 95% confidence intervals.</p