MYC expression in SCLC cell lines by IHC.

<p>Representative MYC IHC staining patterns are shown: <i>A</i>, negative (H196 / H-score 0); <i>B</i>, low MYC (H1963 / H-score 55); <i>C</i>, high MYC (H146 / H-score 140); and <i>D</i>, high MYC (N417 / H-score 230). <i>E</i>, summary of MYC IHC staining by positive cell ratio. The red star indicates cells with <i>MYC</i> gene amplification.</p

Establishment of cisplatin treated cells and the sensitivity to Pladienolide B.

<p><i>A</i>, efficacy of cisplatin in SW1271 and H1048 parental cells. <i>B</i>, efficacy of Pladienolide B in parental cells and cisplatin-treated cells. Percent growth was calculated relative to DMSO-treated control.</p

Efficacy of a spliceosome inhibitor, Pladienolide B, on SCLC cell lines.

<p>Cells were treated with Pladienolide B for 72 hours and the percent growth was calculated relative to the DMSO-treated control. <i>A</i>, cell lines were classified based on the IHC MYC expression status. <i>B</i>, cell lines were classified based on the presence / absence of a previous chemotherapy at the time of cell line establishment.</p

Association between disease status and abnormal FISH marker (>2 copies per cell) for the top 4 markers (CEP3, <i>TP63</i>, CEP6, <i>MYC</i>).

*<p>Adjusted by multiple logistic regression for gender, age, center, current smoking status and histologic grade.</p

Percentage of lesions with abnormal FISH copy numbers according to case or control status (70 subjects).

*<p>Adjusted by multiple logistic regression for gender, age, center, and current smoking status.</p

Receiver operating characteristic curves describing the diagnostic prediction accuracy of three models, demographics alone (plain line, area under the curve (AUC) 73.4%), in combination with cytology (dashed line, AUC 86.6%) or demographics in combination with cytology and 4 FISH biomarker candidates (dotted line, AUC 92.6%).

<p>The demographic information represents gender, age, pack years smoking history, and smoking status.</p

Distribution of lung cancer cases and controls according to demographic and histology variables.

<p>Distribution of lung cancer cases and controls according to demographic and histology variables.</p