Workflow of collection construction and verification.

<p>A. General procedure and logistics of mutant generation, storage and characterization. B. Identification of obtained mutants and mapping of mutants to specific wells by a “Hypercube” Combinatorial Pooling approach, essentially as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099820#pone.0099820-Goodman1" target="_blank">[30]</a>.</p

Summary of collection statistics.

a<p>CDS where no transposon insertion was found in a high complexity random transposon library <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099820#pone.0099820-Canals1" target="_blank">[29]</a>. List of mutants present in our collections. All <i>S</i>. Typhimurium strain 14028s annotated features <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099820#pone.0099820-Jarvik1" target="_blank">[12]</a> are presented along with their status in our single-gene deletion (SGD) mutant collections. Corresponding genes in <i>S</i>. Typhimurium strain LT2 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099820#pone.0099820-McClelland1" target="_blank">[27]</a> that have at least 70% DNA identity and at least 70% coverage are annotated. The SGD collections are listed in genome order, followed by the multi-gene deletion (MGD) collections in genome order. For MGDs, only those primer pairs that resulted in successful deletion in at least one of the two varieties (Kan or Cam) are shown. Primer sequences, their genome locations, and the results of mutant generation, verification, and mapping to 96-well plates are shown. Empty wells are not listed.</p