1 research outputs found
Dual-Oriented Solid-Phase Molecular Imprinting: Toward Selective Artificial Receptors for Recognition of Nucleotides in Water
We
describe the synthesis of water-soluble molecularly imprinted
polymer nanoparticles (MIP-NPs) as a new artificial host receptor
for the recognition of adenosine monophosphate (AMP), used herein,
as a model nucleotide. MIP-NPs were prepared by solid-phase synthesis
on glass beads (GB) using, for the first time, immobilized Fe(III)-chelate
as an affinity ligand to orientate the AMP via its phosphate group.
A polymerizable thymine monomer which can induce complementary base-pairing
with the adenine moiety of the nucleotide was synthesized and incorporated
in the polymerization mixture to constrain the AMP in a dual-orientated
configuration. The MIP-NPs were remarkably selective toward AMP as
they did not bind other nucleotides, GMP, UMP, and CMP. This strategy
of using the phosphate group of AMP as a hinge enables unhindered
pairing of the nucleobase with its corresponding complementary base
monomer and can be extended to the preparation of specific MIP receptors
for other key nucleotides in aqueous conditions