Unidirectional frequency conversion in microring resonators for on-chip frequency-multiplexed single-photon sources

Microring resonators are attractive for low-power frequency conversion via Bragg-scattering four-wave-mixing due to their comb-like resonance spectrum. However, conversion efficiency is limited to 50% due to the equal probability of up- and down-conversion. Here, we demonstrate how two coupled microrings enable highly directional conversion between the spectral modes of one of the rings. An extinction between up- and down-conversion of more than 40 dB is experimentally observed. Based on this method, we propose a design for on-chip multiplexed single-photon sources that allow localized frequency modes to be converted into propagating continuous-mode photon wave packets using a single operation. The key is that frequency conversion works as a switch on both spatial and spectral degrees of freedom of photons if the microring is interferometrically coupled to a bus waveguide. Our numerical results show 99% conversion efficiency into a propagating mode with a wave packet having a 90% overlap with a Gaussian for a ratio between intrinsic and coupling quality factors of 400

Benchmarking five numerical simulation techniques for computing resonance wavelengths and quality factors in photonic crystal membrane line defect cavities

We present numerical studies of two photonic crystal membrane microcavities, a short line-defect cavity with relatively low quality ($Q$) factor and a longer cavity with high $Q$. We use five state-of-the-art numerical simulation techniques to compute the cavity $Q$ factor and the resonance wavelength $\lambda$ for the fundamental cavity mode in both structures. For each method, the relevant computational parameters are systematically varied to estimate the computational uncertainty. We show that some methods are more suitable than others for treating these challenging geometries.Comment: Revised and final version for publication. 28 pages, 10 figures, 7 table

Genetic transformation of Fusarium avenaceum by Agrobacterium tumefaciens mediated transformation and the development of a USER-Brick vector construction system

BACKGROUND: The plant pathogenic and saprophytic fungus Fusarium avenaceum causes considerable in-field and post-field losses worldwide due to its infections of a wide range of different crops. Despite its significant impact on the profitability of agriculture production and a desire to characterize the infection process at the molecular biological level, no genetic transformation protocol has yet been established for F. avenaceum. In the current study, it is shown that F. avenaceum can be efficiently transformed by Agrobacterium tumefaciens mediated transformation. In addition, an efficient and versatile single step vector construction strategy relying on Uracil Specific Excision Reagent (USER) Fusion cloning, is developed. RESULTS: The new vector construction system, termed USER-Brick, is based on a limited number of PCR amplified vector fragments (core USER-Bricks) which are combined with PCR generated fragments from the gene of interest. The system was found to have an assembly efficiency of 97% with up to six DNA fragments, based on the construction of 55 vectors targeting different polyketide synthase (PKS) and PKS associated transcription factor encoding genes in F. avenaceum. Subsequently, the ΔFaPKS3 vector was used for optimizing A. tumefaciens mediated transformation (ATMT) of F. avenaceum with respect to six variables. Acetosyringone concentration, co-culturing time, co-culturing temperature and fungal inoculum were found to significantly impact the transformation frequency. Following optimization, an average of 140 transformants per 10(6) macroconidia was obtained in experiments aimed at introducing targeted genome modifications. Targeted deletion of FaPKS6 (FA08709.2) in F. avenaceum showed that this gene is essential for biosynthesis of the polyketide/nonribosomal compound fusaristatin A. CONCLUSION: The new USER-Brick system is highly versatile by allowing for the reuse of a common set of building blocks to accommodate seven different types of genome modifications. New USER-Bricks with additional functionality can easily be added to the system by future users. The optimized protocol for ATMT of F. avenaceum represents the first reported targeted genome modification by double homologous recombination of this plant pathogen and will allow for future characterization of this fungus. Functional linkage of FaPKS6 to the production of the mycotoxin fusaristatin A serves as a first testimony to this