2,257 research outputs found
Real-time NMR monitoring of biological activities in complex physiological environments
Biological reactions occur in a highly organized spatiotemporal context and with kinetics that are modulated by multiple environmental factors. To integrate these variables in our experimental investigations of 'native' biological activities, we require quantitative tools for time-resolved in situ analyses in physiologically relevant settings. Here, we outline the use of high-resolution NMR spectroscopy to directly observe biological reactions in complex environments and in real-time. Specifically, we discuss how real-time NMR (RT-NMR) methods have delineated insights into metabolic processes, post-translational protein modifications, activities of cellular GTPases and their regulators, as well as of protein folding events.Fil: Smith, Matthew J.. Ontario Cancer Institute; CanadáFil: Marshall, Christopher B.. Ontario Cancer Institute; CanadáFil: Theillet, Francois Xavier. Leibniz Institute of Molecular Pharmacology; AlemaniaFil: Binolfi, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Leibniz Institute of Molecular Pharmacology; AlemaniaFil: Selenko, Philipp. Leibniz Institute of Molecular Pharmacology; AlemaniaFil: Ikura, Mitsuhiko. Ontario Cancer Institute; Canadá. University of Toronto; Canad
Dynamic and Static Magnetic Resonance Angiography of the Supra-aortic Vessels at 3.0 T Intraindividual Comparison of Gadobutrol, Gadobenate Dimeglumine, and Gadoterate Meglumine at Equimolar Dose
Purpose: The purpose of this study was the intraindividual comparison of a 1.0 M and two 0.5 M gadolinium-based contrast agents (GBCA) using equimolar dosing in dynamic and static magnetic resonance angiography (MRA) of the supra-aortic vessels. Materials and Methods: In this institutional review board-approved study, a total of 20 healthy volunteers (mean +/- SD age, 29 +/- 6 years) underwent 3 consecutive supra-aortic MRA examinations on a 3.0 T magnetic resonance system. The order of GBCA (Gadobutrol, Gadobenate dimeglumine, and Gadoterate meglumine) was randomized with a minimum interval of 48 hours between the examinations. Before each examination and 45 minutes after each examination, circulatory parameters were recorded. Total GBCA dose per MRA examination was 0.1 mmol/kg with a 0.03 mmol/kg and 0.07 mmol/kg split for dynamic and static MRA, respectively, injected at a rate of 2 mL/s. Two blinded readers qualitatively assessed static MRA data sets independently using pairwise rankings (superior, inferior, and equal). In addition, quantitative analysis was performed with signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) evaluation as well as vessel sharpness analysis of static MRA using an in-house-developed semiautomated tool. Dynamic MRA was evaluated for maximal SNR. Statistical analysis was performed using the Cohen kappa, the Wilcoxon rank sum tests, and mixed effects models. Results: No significant differences of hemodynamic parameters were observed. In static MRA, Gadobutrol was rated superior to Gadoterate meglumine (P 0.05). Maximal SNR in dynamic MRA using Gadobutrol was significantly higher than both comparators at the level of the proximal and distal internal carotid artery (P < 0.05 and P < 0.05; P < 0.05 and P < 0.05). Conclusions: At equimolar doses, 1.0 M Gadobutrol demonstrates higher SNR/CNR than do Gadobenate dimeglumine and Gadoterate meglumine, with superior image quality as compared with Gadoterate meglumine for dynamic and static carotid MRA. Despite the shortened bolus with Gadobutrol, no blurring of vessel edges was observed
The Rise of the s-Process in the Galaxy
From newly-obtained high-resolution, high signal-to-noise ratio spectra the
abundances of the elements La and Eu have been determined over the stellar
metallicity range -3<[Fe/H]<+0.3 in 159 giant and dwarf stars. Lanthanum is
predominantly made by the s-process in the solar system, while Eu owes most of
its solar system abundance to the r-process. The changing ratio of these
elements in stars over a wide metallicity range traces the changing
contributions of these two processes to the Galactic abundance mix. Large
s-process abundances can be the result of mass transfer from very evolved
stars, so to identify these cases, we also report carbon abundances in our
metal-poor stars. Results indicate that the s-process may be active as early as
[Fe/H]=-2.6, alalthough we also find that some stars as metal-rich as [Fe/H]=-1
show no strong indication of s-process enrichment. There is a significant
spread in the level of s-process enrichment even at solar metallicity.Comment: 64 pages, 15 figures; ApJ 2004 in pres
Neutron-Capture Elements in the Early Galaxy: Insights from a Large Sample of Metal-Poor Giants
New abundances for neutron-capture (n-capture) elements in a large sample of
metal-poor giants from the Bond survey are presented. The spectra were acquired
with the KPNO 4-m echelle and coude feed spectrographs, and have been analyzed
using LTE fine-analysis techniques with both line analysis and spectral
synthesis. Abundances of eight n-capture elements (Sr, Y, Zr, Ba, La, Nd, Eu,
Dy) in 43 stars have been derived from blue (lambda = 4070--4710, R~20,000, S/N
ratio~100-200) echelle spectra and red (lambda = 6100--6180, R~22,000, S/N
ratio~100-200) coude spectra, and the abundance of Ba only has been derived
from the red spectra for an additional 27 stars. Overall, the abundances show
clear evidence for a large star-to-star dispersion in the heavy element-to-iron
ratios. The new data also confirm that at metallicities [Fe/H] <~ --2.4, the
abundance pattern of the heavy (Z >= 56) n-capture elements in most giants is
well-matched to a scaled Solar System r-process nucleosynthesis pattern. The
onset of the main r-process can be seen at [Fe/H] ~ --2.9. Contributions from
the s-process can first be seen in some stars with metallicities as low as
[Fe/H] ~ --2.75, and are present in most stars with metallicities [Fe/H] >
--2.3. The lighter n-capture elements (Sr-Y-Zr) are enhanced relative to the
heavier r-process element abundances. Their production cannot be attributed
solely to any combination of the Solar System r- and main s-processes, but
requires a mixture of material from the r-process and from an additional
n-capture process which can operate at early Galactic time.Comment: Text + 5 Tables and 11 Figures: Submitted to the Astrophysical
Journa
The r-Process Enriched Low Metallicity Giant HD 115444
New high resolution, very high signal-to-noise spectra of ultra-metal-poor
(UMP) giant stars HD 115444 and HD 122563 have been gathered with the
High-Resolution Echelle Spectrometer of the McDonald Observatory 2.7m
Telescope. With these spectra, line identification and model atmosphere
analyses have been conducted, emphasizing the neutron-capture elements. Twenty
elements with Z > 30 have been identified in the spectrum of HD 115444. This
star is known to have overabundances of the neutron-capture elements, but it
has lacked a detailed analysis necessary to compare with nucleosynthesis
predictions. The new study features a line-by-line differential abundance
comparison of HD 115444 with the bright, well-studied halo giant HD 122563. For
HD 115444, the overall metallicity is [Fe/H]~ -3.0. The abundances of the light
and iron-peak elements generally show the same pattern as other UMP stars (e.g.
overdeficiencies of manganese and chromium, overabundances of cobalt), but the
differential analysis indicates several nucleosynthesis signatures that are
unique to each star.Comment: To Appear in the Astrophysical Journa
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