The mediterranean sea we want

open58siThis paper presents major gaps and challenges for implementing the UN Decade of Ocean Science for Sustainable Development (2021-2030) in the Mediterranean region. The authors make recommendations on the scientific knowledge needs and co-design actions identified during two consultations, part of the Decade preparatory-phase, framing them in the Mediterranean Sea’s unique environmental and socio-economic perspectives. According to the ‘Mediterranean State of the Environment and Development Report 2020’ by the United Nations Environment Programme Mediterranean Action Plan and despite notable progress, the Mediterranean region is not on track to achieve and fully implement the Sustainable Development Goals of Agenda 2030. Key factors are the cumulative effect of multiple human-induced pressures that threaten the ecosystem resources and services in the global change scenario. The basin, identified as a climate change vulnerability hotspot, is exposed to pollution and rising impacts of climate change. This affects mainly the coastal zones, at increasing risk of extreme events and their negative effects of unsustainable management of key economic assets. Transitioning to a sustainable blue economy is the key for the marine environment’s health and the nourishment of future generations. This challenging context, offering the opportunity of enhancing the knowledge to define science-based measures as well as narrowing the gaps between the Northen and Southern shores, calls for a joint (re)action. The paper reviews the state of the art of Mediterranean Sea science knowledge, sets of trends, capacity development needs, specific challenges, and recommendations for each Decade’s societal outcome. In the conclusions, the proposal for a Mediterranean regional programme in the framework of the Ocean Decade is addressed. The core objective relies on integrating and improving the existing ocean-knowledge, Ocean Literacy, and ocean observing capacities building on international cooperation to reach the “Mediterranean Sea that we want”.openCappelletto M.; Santoleri R.; Evangelista L.; Galgani F.; Garces E.; Giorgetti A.; Fava F.; Herut B.; Hilmi K.; Kholeif S.; Lorito S.; Sammari C.; Lianos M.C.; Celussi M.; D'alelio D.; Francocci F.; Giorgi G.; Canu D.M.; Organelli E.; Pomaro A.; Sannino G.; Segou M.; Simoncelli S.; Babeyko A.; Barbanti A.; Chang-Seng D.; Cardin V.; Casotti R.; Drago A.; Asmi S.E.; Eparkhina D.; Fichaut M.; Hema T.; Procaccini G.; Santoro F.; Scoullos M.; Solidoro C.; Trincardi F.; Tunesi L.; Umgiesser G.; Zingone A.; Ballerini T.; Chaffai A.; Coppini G.; Gruber S.; Knezevic J.; Leone G.; Penca J.; Pinardi N.; Petihakis G.; Rio M.-H.; Said M.; Siokouros Z.; Srour A.; Snoussi M.; Tintore J.; Vassilopoulou V.; Zavatarelli M.Cappelletto M.; Santoleri R.; Evangelista L.; Galgani F.; Garces E.; Giorgetti A.; Fava F.; Herut B.; Hilmi K.; Kholeif S.; Lorito S.; Sammari C.; Lianos M.C.; Celussi M.; D'alelio D.; Francocci F.; Giorgi G.; Canu D.M.; Organelli E.; Pomaro A.; Sannino G.; Segou M.; Simoncelli S.; Babeyko A.; Barbanti A.; Chang-Seng D.; Cardin V.; Casotti R.; Drago A.; Asmi S.E.; Eparkhina D.; Fichaut M.; Hema T.; Procaccini G.; Santoro F.; Scoullos M.; Solidoro C.; Trincardi F.; Tunesi L.; Umgiesser G.; Zingone A.; Ballerini T.; Chaffai A.; Coppini G.; Gruber S.; Knezevic J.; Leone G.; Penca J.; Pinardi N.; Petihakis G.; Rio M.-H.; Said M.; Siokouros Z.; Srour A.; Snoussi M.; Tintore J.; Vassilopoulou V.; Zavatarelli M

Biomethane production from tobacco plants with modified cell wall

Plant biomasses represent an abundant source of lignocellulosic material for renewable biofuel production. The conversion of plant cell wall polysaccharides into simple fermentable sugars (saccharification) is typically regarded as a major bottleneck for the bioconversion process. The recalcitrance to saccharification is largely due to the complex structure of the cell wall that is composed of a cellulose-hemicellulose network embedded in a matrix of pectin that limits the exposure of cellulose to hydrolysis. It has been previously demonstrated that the modification of homogalacturonan, the main component of pectin, by the expression of an attenuated variant of the polygalacturonase II of the fungus Aspergillus niger (hereafter named PG plants), improves enzymatic saccharification with respect to the unmodified plants. In this work, two transgenic tobacco PG plants (lines PG16 and PG7, expressing respectively high and low level of the transgene), were used to evaluate the effect of cell wall modifications on anaerobic fermentation of plant tissues into methane gas. To this aim, samples of both transgenic and wild type plants were incubated (at 35°C) in closed reactors in the presence of an anaerobic sludge as inoculum. Control tests lacking plant biomass or containing a corresponding amount of glucose (instead of plant biomass) were also setup. Each condition was tested in triplicate to evaluate its reproducibility. The reactors were analyzed at regular intervals for organic acids (the main intermediates resulting from the fermentation of saccharification products) and methane gas, the end-product of anaerobic degradation of plant biomasses. The production rate of organic acids (namely acetate, propionate, and butyrate) with PG16 modified tobacco was substantially higher than with the wild-type, confirming that the expression of AnPGII can enhance the rate of saccharification. Interestingly, the final conversion yield of tobacco plants (both modified and wild-type) into methane was over 90% (on a COD basis) and only slightly lower than that observed with pure glucose. In conclusion, these results suggest that residuals from tobacco plants have a high potential for conversion into biofuels

Morpho-Physiological and Metal Accumulation Responses of Hemp Plants (Cannabis Sativa L.) Grown on Soil from an Agro-Industrial Contaminated Area

Hemp is a promising plant for phytomanagement. The possibility to couple soil restoration to industrial crop cultivation makes this plant attractive for the management of contaminated sites. In this trial, Cannabis sativa L. plants were grown in a greenhouse on soils from two sites of “Valle del Sacco” (Lazio Region, Italy), a wide area contaminated by agro-industrial activities. One site was representative of moderate and diffuse metal(loid) multi-contamination, above the Italian concentration limit for agriculture (MC—moderately contaminated). The second site showed a metal(loid) content below the aforementioned limit, as a typical background level of the district (C—control). After 90 days, biometric and physiological parameters revealed satisfactory growth in both soil types. MC-grown plants showed a slight, but significant reduction in leaf area, root, and leaf biomass compared with C-grown plants. Chlorophyll content and chlorophyll fluorescence parameters, namely the quantum yield of primary photochemistry (Fv/Fm) and the Performance Index (PIABS), confirmed the good physiological status of plants in both soils. Metal(loid) analyses revealed that As, V, and Pb accumulated only in the roots with significant differences in MC- and C-grown plants, while Zn was found in all organs. Overall, preliminary results showed a satisfactorily growth coupled with the restriction of toxic metal translocation in MC-grown hemp plants, opening perspectives for the phytomanagement of moderately contaminated areas

Impaired Cuticle Functionality and Robust Resistance to <i>Botrytis cinerea </i>in <i>Arabidopsis thaliana</i> Plants With Altered Homogalacturonan Integrity Are Dependent on the Class III Peroxidase AtPRX71

Pectin is a major cell wall component that plays important roles in plant development and response to environmental stresses. Arabidopsis thaliana plants expressing a fungal polygalacturonase (PG plants) that degrades homogalacturonan (HG), a major pectin component, as well as loss-of-function mutants for QUASIMODO2 (QUA2), encoding a putative pectin methyltransferase important for HG biosynthesis, show accumulation of reactive oxygen species (ROS), reduced growth and almost complete resistance to the fungal pathogen Botrytis cinerea. Both PG and qua2 plants show increased expression of the class III peroxidase AtPRX71 that contributes to their elevated ROS levels and reduced growth. In this work, we show that leaves of PG and qua2 plants display greatly increased cuticle permeability. Both increased cuticle permeability and resistance to B. cinerea in qua2 are suppressed by loss of AtPRX71. Increased cuticle permeability in qua2, rather than on defects in cuticle ultrastructure or cutin composition, appears to be dependent on reduced epidermal cell adhesion, which is exacerbated by AtPRX71, and is suppressed by the esmeralda1 mutation, which also reverts the adhesion defect and the resistant phenotype. Increased cuticle permeability, accumulation of ROS, and resistance to B. cinerea are also observed in mutants lacking a functional FERONIA, a receptor-like kinase thought to monitor pectin integrity. In contrast, mutants with defects in other structural components of primary cell wall do not have a defective cuticle and are normally susceptible to the fungus. Our results suggest that disrupted cuticle integrity, mediated by peroxidase-dependent ROS accumulation, plays a major role in the robust resistance to B. cinerea of plants with altered HG integrity

Uso di piante con un ridotti livelli di omogalatturonano de-esterificato nella parete cellulare o parti di esse per migliorare la saccarificazione di biomasse vegetali

L'invenzione riguarda un'invenzione concernente un procedimento per una migliore conversione in zuccheri fermentabili di biomasse lignocellulosiche, un processo chiamato saccarificazione, che permette poi la produzione, anche su scala industriale, di tutto ciò che può essere ottenuto per via fermentativa, ivi compreso l'etanolo (bioetanolo). Riguarda l'espressione stabile in piante di enzimi pectionolitici e di inibitori della pectina metilesterasi per aumentaer la degradabilità dei tessuti vegetali tramite digestione enzimatica e quindi l'efficienza di saccarificazione