Contrived blood samples spiked with a range of elevated levels of AGP, CRP, HRP2 and sTfR.

Contrived blood samples spiked with a range of elevated levels of AGP, CRP, HRP2 and sTfR.</p

Schematic illustrating the plasma collection disc in operation.

The schematic illustrates a single PCD. Photos on the left show the PCD with the the filter disc placed on a manifold for multiple samples and the addition of 35 μL of whole blood to each disc (top), a PDC detail view prior to whole blood being added (middle), and the discs after blood has been absorbed and finally the plasma collection disc exposed after the whole blood treated filter has been removed (bottom). The disc is removed with sterile forceps and is ready for use or can be stored until required.</p

Summary statistics and cross-laboratory coefficients of variation (CV) for plasma, DBS and PCD.

Summary statistics and cross-laboratory coefficients of variation (CV) for plasma, DBS and PCD.</p

Fig 3 -

A. Lin’s concordance correlation coefficient (CCC) plots comparing the analyte measurements in paired wet plasma (x-axes) and DBS samples (y-axes). AGP, α-1-acid glycoprotein; CRP, C-reactive protein; FER, ferritin; HRP2, histidine rich protein 2; RBP4, retinol binding protein 4; sTfR, soluble transferrin receptor; Tg, thyroglobulin; DBS, dried blood spot; rho_c, CCC. B, Lin’s CCC plots comparing the analyte measurements in paired wet plasma (x-axes) and the adjusted PCD (y-axes). AGP, α-1-acid glycoprotein; CRP, C-reactive protein; FER, ferritin; HRP2, histidine rich protein 2; RBP4, retinol binding protein 4; sTfR, soluble transferrin receptor; Tg, thyroglobulin; DBS, dried blood spot; rho_c, CCC.</p

The comparison of results for plasma, DBS and the volume adjusted PCD eluates using <i>R</i>_<i>s</i> and recovery of expected plasma values.

The comparison of results for plasma, DBS and the volume adjusted PCD eluates using Rs and recovery of expected plasma values.</p

Sample panels and experiment design.

Sample panels and experiment design.</p

Fig 4 -

A. Bland Altman plots of the difference in results derived from wet plasma versus DBS types measured using the 7-plex assay. Average of the wet plasma and dry sample type (x-axes) are plotted against the difference between measurements from the 7-Plex assay for paired wet plasma and dry sample types (y-axes). B. Bland Altman plots of the difference in results derived from wet plasma versus the adjusted PCD. For both Figures, the horizontal lines indicate line of perfect agreement (green), mean (purple), and ± 2standard deviations of the difference (red). AGP, α-1-acid glycoprotein; CRP, C-reactive protein; FER, ferritin; HRP2, histidine rich protein 2; RBP4, retinol binding protein 4; sTfR, soluble transferrin receptor; Tg, thyroglobulin; DBS, dried blood spot.</p