PrP^sc detection in posterior brainstem and lymphoid tissue of goats from 8 classical scrapie infected herds.

*<p>excluding the index case.</p>**<p>Mesenteric lymph node. Official screening tests on posterior brain stem were only performed in animals older than 18 months by a state accredited laboratory. In parallel, PrP^Sc detection by immunohistochemistry and ELISA (Biorad TeSeE sheep/goat®,) was performed in our premises. In some cases samples were not tested because they were either missing or of insufficient amount.</p

Estimated detection sensitivity of scrapie infected herds in a commercial population through <i>post-mortem</i> testing programs.

<p>The performances of different TSE surveillance programs at population level were estimated based on the French goat population as an example, using a Monte Carlo simulation model. The model relied on the real structure and management practises of the French goat population and on the measured performances (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054911#pone-0054911-t002" target="_blank">table 2</a>) of scrapie detection by post mortem PrP^Sc detection assays. The proportion of scrapie infected herds detected at abattoir (white boxes), rendering (black boxes) or in both streams together (grey boxes). In all cases PrP^Sc is carried out using ELISA (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054911#pone-0054911-t002" target="_blank">table 2</a>). (A–B) 100% of animals eliminated from the commercial population are tested on posterior brainstem (A) or on palatine tonsil (B). (C–D) 10 000 animals are tested at abattoir and 10 000 animals are tested at rendering plant on either posterior brainstem (C) or palatine tonsil. (D).</p

Concordance between Immunohistochemistry and ELISA (Biorad TeSeE sheep/goat®) PrP^Sc detection results in tissues from classical scrapie infected goats.

*<p>Mesenteric lymph node.</p