Long-term effects of maternal separation on chronic stress response suppressed by amitriptyline treatment

The early-life environment has many long-term effects on mammals. Maternal interaction and early stressful events may affect regulation of the HPA axis during adulthood, leading to differential glucocorticoid secretion in response to stressful situations. These adverse experiences during postnatal development may even sensitize specific neurocircuits to subsequent stressors. Later in life, the overreaction of the HPA axis to stress can constitute a risk factor for metabolic and mental diseases. As tricyclic antidepressants are known to correct glucocorticoid hypersecretion during depression, we treated maternally separated animals with amitriptyline, at a lower dose than habitually used in depression models, to prevent the response to chronic stress during adulthood. Male Wistar rats were separated from the mother for 4.5 h every day for the first 3 weeks of life. From postnatal day 50, animals were subjected to chronic variable stress during 24 d (five types of stressors at different times of day). During the stress, protocol rats were orally administered amitriptyline (5 mg/kg) daily. We observed that maternal separation caused a reduction in plasma ACTH levels (p < 0.05), but evoked hypersecretion of corticosterone (p < 0.05) when it was combined with stress in adulthood. This rise was completely prevented by antidepressant treatment with amitriptyline.Fil: Cotella, Evelin Mariel. Universidad Nacional de Cordoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Fisiologia Animal; ArgentinaFil: Mestres Lascano, Ivan. Universidad Nacional de Cordoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Fisiologia Animal; ArgentinaFil: Franchioni, L.. Hospital de Niños de La Santísima Trinidad; ArgentinaFil: Levin, Gloria Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Suarez, M. M.. Universidad Nacional de Cordoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Fisiologia Animal; Argentin

Towards holistic power distribution system validation and testing - an overview and discussion of different possibilities

Renewable energy sources are key enablers to decrease greenhouse gas emissions and to cope with the anthropogenic global warming. Their intermittent behaviour and limited storage capabilities present challenges to power system operators in maintaining the high level of power quality and reliability. However, the increased availability of advanced automation and communication technologies has provided new intelligent solutions to face these challenges. Previous work has presented various new methods to operate highly interconnected power grids with corresponding components in a more effective way. As a consequence of these developments the traditional power system is transformed into a cyber-physical system, a smart grid. Previous and ongoing research activities have mainly focused on validating certain aspects of smart grids, but until now no integrated approach for analysing and evaluating complex configurations in a cyber-physical systems manner is available. This paper tackles this issue and addresses system validation approaches for smart grids. Different approaches for different stages in the design, development, and roll-out phase of smart grid solutions and components are discussed. Finally, future research directions are analysed