Volatile Composition and Biological Activity of Key Lime Citrus aurantifolia Essential Oil

The essential oil of Citrus aurantifolia (Christm) Swingle fruits (limes) was studied for its potential spasmolytic effects in relation to its chemical composition. The essential oil, extracted by hydrodistillation (HD), was analyzed by GC-FID and GC-MS. The antispasmodic activity was evaluated on isolated rabbit jejunum, aorta and uterus. The results indicated that the essential oil of C aurantifolia possesses important spasmolytic properties, which are likely to be due to its major constituents, limonene (58.4%), β-pinene (15.4%), γ-terpinene (8.5%), and citral (4.4%)

Pulmonary embolism post-Covid-19 infection. Physiopathological mechanisms and vascular damage biomarkers

Covid-19 infection is characterized by several acute complications, as well long-term sequelae, mostly sustained by endothelial dysfunction; several studies show that complications as pulmonary embolism (PE) are described both in the acute phase and after negativization. Aim of research was to evaluate anthropometric, bio-humoral, instrumental parameters in a group of patients affected by PE after recent Covid-19 infection compared to PE patients without previous Covid-19 infection. We enrolled 72 consecutive patients (35M, 37F) with acute PE, distinguished in relation to previous acute Covid-19 infection: 54 pts without previous acute Covid-19 infection and 18 pts with previous Covid-19 infection within negativity at least 2 months before PE diagnosis; 44 healthy subjects (21M, 23F) were recruited as control group. Patients who had previously developed Covid-19 needed hospitalization in high percentage (84%); this group showed significantly higher prevalence of diabetes mellitus than Covid-19-free PE patients, reduced serum levels of C-reactive protein, sST2 and PESI score. In post-Covid-19 PE group, we observed higher mean IMPROVE risk score, whereas in Covid-19-free group lower P/F ratio, higher radiological severity, and worse PESI score and severity index. Covid-19 infection affects not just the lung parenchyma but also other organs; endothelial damage plays pivotal role in long-term alterations; in high thrombotic risk group (recent hospitalization due to acute Covid-19 infection), we have described thrombotic complications characterized by persistent prothrombotic state after recovery, highlighted by well-known markers as PCR and D-Dimer as well as novel vascular marker (sST2)

Analysis of the miRNA expression from the adipose tissue surrounding the adrenal neoplasia

BackgroundPrimary aldosteronism (PA) is characterized by several metabolic changes such as insulin resistance, metabolic syndrome, and adipose tissue (AT) inflammation. Mi(cro)RNAs (miRNAs) are a class of non-coding small RNA molecules known to be critical regulators in several cellular processes associated with AT dysfunction. The aim of this study was to evaluate the expression of some miRNAs in visceral and subcutaneous AT in patients undergoing adrenalectomy for aldosterone-secreting adrenal adenoma (APA) compared to the samples of AT obtained in patients undergoing adrenalectomy for non-functioning adrenal mass (NFA).MethodsThe quantitative expression of selected miRNA using real-time PCR was analyzed in surrounding adrenal neoplasia, peri-renal, and subcutaneous AT samples of 16 patients with adrenalectomy (11 patients with APA and 5 patients with NFA).ResultsReal-time PCR cycles for miRNA-132, miRNA-143, and miRNA-221 in fat surrounding adrenal neoplasia and in peri-adrenal AT were significantly higher in APA than in patients with NFA. Unlike patients with NFA, miRNA-132, miRNA-143, miRNA-221, and miRNA-26b were less expressed in surrounding adrenal neoplasia AT compared to subcutaneous AT in patients with APA.ConclusionThis study, conducted on tissue expression of miRNAs, highlights the possible pathophysiological role of some miRNAs in determining the metabolic alterations in patients with PA

Prilog poznavanju kseno-raznolikosti životinja duž obale Kalabrije (južna Italija, srednji Mediteran)

In this paper a contribution to the knowledge of marine and brackish water alien species recorded along the Calabrian coasts (Southern Italy, Central Mediterranean) during the period 2000-2013 is given. The study area is located in the center of the Mediterranean. Records of alien species come from 13 years of both field and opportunistic fishing surveys. Also a bibliographical search in the scientific literature and public and private archives was performed. Eighteen of marine alien species recorded: 1 cnidarian, 11 molluscs, 3 crustaceans, and 4 bony fishes; in addition to these, was considered also the presence of four bony fish, that have naturally spread into the Mediterranean: Sphoeroides pachygaster, Gymnothorax moringa, Pseunes pellucidus and Zenopsis conchifera. The highest number of records comes from the Messina Strait. The most common and widest observed species were Percnon gibbesi, Callinectes sapidus, Fistularia commersonii and Procambarus clarckii. The record of Ruditapes philippinarum in the Foce Crati is the first for the Ionian Sea and for the Central Mediterranean. Gymnothorax moringa is here recorded for the first time in the Mediterranean.Ovaj rad predstavlja prilog poznavanju stranih vrsta pronađenih u morskoj i boćatoj vodi duž Kalabrijske obale (južna Italija, Središnji Mediteran) u razdoblju od 2000. do 2013. godine. Područje istraživanja se nalazi u središtu Mediterana. Evidencija stranih vrsta je proizišla iz 13 godina terenskog rada i oportunističkih istraživanja. Također su izvršena bibliografska pretraživanja u znanstvenoj literaturi u javnim i privatnim arhivima. Ukupno je zabilježeno 18 morskih stranih vrsta: 1 cnidaria, 11 mekušaca, 3 raka i 4 ribe koštunjače koje su se prirodno proširile u Mediteranu: Sphoeroides pachygaster, Gymnothorax moringa, Pseunes pellucidus i Zenopsis conchifera. Najveći broj nalaza dolazi iz Mesinskog tjesnaca. Najčešće i najšire promatrane vrste su Percnon gibbesi, Callinectes sapidus, Fistularia commersonii i Procambarus clarkii. Zapis o nalazu vrste Ruditapes philippinarum, kod mjesta Foce Crati, je prvi za Jonsko more i središnji Mediteran. Gymnothorax moringaje po prvi put zabilježena u Mediteranu

A fast-rotator post-starburst galaxy quenched by supermassive black-hole feedback at z=3

There is compelling evidence that the most massive galaxies in the Universe stopped forming stars due to the time-integrated feedback from their central super-massive black holes (SMBHs). However, the exact quenching mechanism is not yet understood, because local massive galaxies were quenched billions of years ago. We present JWST/NIRSpec integral-field spectroscopy observations of GS-10578, a massive, quiescent galaxy at redshift z=3.064. From the spectrum we infer that the galaxy has a stellar mass of $M_*=1.6\pm0.2 \times 10^{11}$ MSun and a dynamical mass $M_{\rm dyn}=2.0\pm0.5 \times 10^{11}$ MSun. Half of its stellar mass formed at z=3.7-4.6, and the system is now quiescent, with the current star-formation rate SFR<9 MSun/yr. We detect ionised- and neutral-gas outflows traced by [OIII] emission and NaI absorption. Outflow velocities reach $v_{\rm out}\approx$1,000 km/s, comparable to the galaxy escape velocity and too high to be explained by star formation alone. GS-10578 hosts an Active Galactic Nucleus (AGN), evidence that these outflows are due to SMBH feedback. The outflow rates are 0.14-2.9 and 30-300 MSun/yr for the ionised and neutral phases, respectively. The neutral outflow rate is ten times higher than the SFR, hence this is direct evidence for ejective SMBH feedback, with mass-loading capable of interrupting star formation by rapidly removing its fuel. Stellar kinematics show ordered rotation, with spin parameter $\lambda_{Re}=0.62\pm0.07$, meaning GS-10578 is rotation supported. This study shows direct evidence for ejective AGN feedback in a massive, recently quenched galaxy, thus clarifying how SMBHs quench their hosts. Quenching can occur without destroying the stellar disc.Comment: 40 pages, 9 figures, submitted to Nat. Ast., comments welcome

New Mediterranean marine biodiversity records (December, 2013)

Based on recent biodiversity studies carried out in different parts of the Mediterranean, the following 19 species are included as new records on the floral or faunal lists of the relevant ecosystems: the green algae Penicillus capitatus (Maltese waters); the nemertean Am- phiporus allucens (Iberian Peninsula, Spain); the salp Salpa maxima (Syria); the opistobranchs Felimida britoi and Berghia coerulescens (Aegean Sea, Greece); the dusky shark Carcharhinus obscurus (central-west Mediterranean and Ionian Sea, Italy); Randall’s threadfin bream Nemipterus randalli, the broadbanded cardinalfish Apogon fasciatus and the goby Gobius kolombatovici (Aegean Sea, Turkey); the reticulated leatherjack Stephanolepis diaspros and the halacarid Agaue chevreuxi (Sea of Marmara, Turkey); the slimy liagora Ganon- ema farinosum, the yellowstripe barracuda Sphyraena chrysotaenia, the rayed pearl oyster Pinctada imbricata radiata and the Persian conch Conomurex persicus (south-eastern Kriti, Greece); the blenny Microlipophrys dalmatinus and the bastard grunt Pomadasys incisus (Ionian Sea, Italy); the brown shrimp Farfantepenaeus aztecus (north-eastern Levant, Turkey); the blue-crab Callinectes sapidus (Corfu, Ionian Sea, Greece). In addition, the findings of the following rare species improve currently available biogeographical knowledge: the oceanic pufferfish Lagocephalus lagocephalus (Malta); the yellow sea chub Kyphosus incisor (Almuñécar coast of Spain); the basking shark Cetorhinus maximus and the shortfin mako Isurus oxyrinchus (north-eastern Levant, Turkey).peer-reviewe

JADES: Probing interstellar medium conditions at z∼5.5−9.5z\sim5.5-9.5 with ultra-deep JWST/NIRSpec spectroscopy

We present emission line ratios from a sample of 26 Lyman break galaxies from $z\sim5.5-9.5$ with $-17.0<M_{1500}<-20.4$, measured from ultra-deep JWST/NIRSpec MSA spectroscopy from JADES. We use 28 hour deep PRISM/CLEAR and 7 hour deep G395M/F290LP observations to measure, or place strong constraints on, ratios of widely studied rest-frame optical emission lines including H$\alpha$, H$\beta$, [OII] $\lambda\lambda$3726,3729, [NeIII] $\lambda$3869, [OIII] $\lambda$4959, [OIII] $\lambda$5007, [OI] $\lambda$6300, [NII] $\lambda$6583, and [SII] $\lambda\lambda$6716,6731 in individual $z>5.5$ spectra. We find that the emission line ratios exhibited by these $z\sim5.5-9.5$ galaxies occupy clearly distinct regions of line-ratio space compared to typical z~0-3 galaxies, instead being more consistent with extreme populations of lower-redshift galaxies. This is best illustrated by the [OIII]/[OII] ratio, tracing interstellar medium (ISM) ionisation, in which we observe more than half of our sample to have [OIII]/[OII]>10. Our high signal-to-noise spectra reveal more than an order of magnitude of scatter in line ratios such as [OII]/H$\beta$ and [OIII]/[OII], indicating significant diversity in the ISM conditions within the sample. We find no convincing detections of [NII] in our sample, either in individual galaxies, or a stack of all G395M/F290LP spectra. The emission line ratios observed in our sample are generally consistent with galaxies with extremely high ionisation parameters (log $U\sim-1.5$), and a range of metallicities spanning from $\sim0.1\times Z_\odot$ to higher than $\sim0.3\times Z_\odot$, suggesting we are probing low-metallicity systems undergoing periods of rapid star-formation, driving strong radiation fields. These results highlight the value of deep observations in constraining the properties of individual galaxies, and hence probing diversity within galaxy population.Comment: 20 pages, 9 figures, submitted to Astronomy & Astrophysics, updated values in table

JADES NIRSpec Spectroscopy of GN-z11: Lyman-α\alpha emission and possible enhanced nitrogen abundance in a z=10.60z=10.60 luminous galaxy

We present JADES JWST/NIRSpec spectroscopy of GN-z11, the most luminous candidate $z>10$ Lyman break galaxy in the GOODS-North field with $M_{UV}=-21.5$. We derive a redshift of $z=10.603$ (lower than previous determinations) based on multiple emission lines in our low and medium resolution spectra over $0.8-5.3\,\mu$m. We significantly detect the continuum and measure a blue rest-UV spectral slope of $\beta=-2.4$. Remarkably, we see spatially-extended Lyman-$\alpha$ in emission (despite the highly-neutral IGM expected at this early epoch), offset 555 km/s redward of the systemic redshift. From our measurements of collisionally-excited lines of both low- and high-ionization (including [O II] $\lambda3727$, [Ne III] $\lambda 3869$ and C III] $\lambda1909$) we infer a high ionization parameter ($\log U\sim -2$). We detect the rarely-seen N IV] $\lambda1486$ and N III]$\lambda1748$ lines in both our low and medium resolution spectra, with other high ionization lines seen in low resolution spectrum such as He II (blended with O III]) and C IV (with a possible P-Cygni profile). Based on the observed rest-UV line ratios, we cannot conclusively rule out photoionization from AGN. The high C III]/He II ratios, however, suggest a likely star-formation explanation. If the observed emission lines are powered by star formation, then the strong N III] $\lambda1748$ observed may imply an unusually high $N/O$ abundance. Balmer emission lines (H$\gamma$, H$\delta$) are also detected, and if powered by star formation rather than an AGN we infer a star formation rate of $\sim 20-30 M_{\odot}\,\rm yr^{-1}$ (depending on the IMF) and low dust attenuation. Our NIRSpec spectroscopy confirms that GN-z11 is a remarkable galaxy with extreme properties seen 430 Myr after the Big Bang.Comment: Submitted to Astronomy & Astrophysics, 14 pages, 9 figure

Application of enhanced recovery after surgery (ERAS) protocols in adrenal surgery: A retrospective, preliminary analysis

Background: The present study was conducted to evaluate the impact of enhanced recovery after surgery (ERAS) pathway in patients undergoing laparoscopic adrenalectomy (LA) for primary and secondary adrenal disease, in reducing the length of primary hospital stay and return to daily activities. Materials and methods: This retrospective study was carried out on 61 patients who underwent LA. A total of 32 patients formed the ERAS group. A total of 29 patients received conventional perioperative care and were assigned as the control group. Groups were compared in terms of patient's characteristics (sex, age, pre-operative diagnosis, side of tumour, tumour size and co-morbidities), post-operative compliance (anaesthesia time, operative time, post-operative stay, post-operative numeric rating scale (NRS) score, analgesic assumption and days to return to daily activities) and post-operative complications. Results: No significant differences in anaesthesia time (P = 0.4) and operative time (P = 0.6) were reported. NRS score 24 h postoperatively was significantly lower in the ERAS group (P < 0.05). The analgesic assumption in post-operative period in the ERAS group was lower (P < 0.05). ERAS protocol led to a significantly shorter length of post-operative stay (P < 0.05) and to return to daily activities (P < 0.05). No differences in peri-operative complications were reported. Discussion: ERAS protocols seem safe and feasible, potentially improving perioperative outcomes of patients undergoing LA, mainly improving pain control, hospital stay and return to daily activities. Further studies are needed to investigate overall compliance with ERAS protocols and their impact on clinical outcomes