Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil

Submitted by Luciana Ferreira ([email protected]) on 2018-07-16T14:11:26Z No. of bitstreams: 2 Artigo - Francesca Guaracyaba Garcia Chapadense - 2015.pdf: 805837 bytes, checksum: 9ee7c0062351769af60e6e5541cdcd10 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira ([email protected]) on 2018-07-17T14:09:29Z (GMT) No. of bitstreams: 2 Artigo - Francesca Guaracyaba Garcia Chapadense - 2015.pdf: 805837 bytes, checksum: 9ee7c0062351769af60e6e5541cdcd10 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-07-17T14:09:29Z (GMT). No. of bitstreams: 2 Artigo - Francesca Guaracyaba Garcia Chapadense - 2015.pdf: 805837 bytes, checksum: 9ee7c0062351769af60e6e5541cdcd10 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-10The mosquito Aedes aegypti has evolved resistance to pyrethroid insecticides. The present study evaluated Ae. aegypti from Goiânia for the resistant phenotype and for mutations associated with resistance. Methods: Insecticide dose-response bioassays were conducted on mosquitoes descended from fi eld-collected eggs, and polymerase chain reaction (PCR) was used to genotype 90 individuals at sites implicated in pyrethroid resistance. Results: All mosquito populations displayed high levels of resistance to deltamethrin, as well as high frequencies of the 1016Ile kdr and 1534Cys kdr mutations. Conclusions: Aedes aegypti populations in the Goiânia area are highly resistant to deltamethrin, presumably due to high frequencies of kdr (knockdown-resistance) mutations