3 research outputs found
Detection of live <i>Salmonella</i> in artificially contaminated food samples (with live and dead cells) by vPCR method.
<p>Detection of live <i>Salmonella</i> in artificially contaminated food samples (with live and dead cells) by vPCR method.</p
Effect of PEMAX on 10<sup>7</sup> dead <i>Salmonella</i> cells in spiked supernatants of the food enrichment broth (FEB).
<p>Effect of PEMAX on 10<sup>7</sup> dead <i>Salmonella</i> cells in spiked supernatants of the food enrichment broth (FEB).</p
Effect of PEMAX on serial dilutions of live <i>Salmonella</i> cells in the presence of dead cells.
<p>Serial dilutions of live <i>Salmonella</i> cells (5.0×10<sup>7</sup> to 5.0×10<sup>3</sup> cfu) in the presence of 5.0×10<sup>7</sup> dead <i>Salmonella</i> cells were treated with 0 μM (white bars) or 100 μM (grey bars) PEMAX followed by real-time PCR. The control samples contains either 5.0×10<sup>7</sup> live or dead cells (no mixed population). Means, standard deviations (n = 4) and the linear regression equation and R<sup>2</sup> value of PEMAX treated samples are depicted. * In 3 of 4 dead samples treated with PEMAX no PCR signal were detected, therefore only the PCR signal of 1 sample is indicated in the diagram.</p