Antibody responses to <i>Ae</i>. <i>aegypti</i> and <i>Ae</i>. <i>polynesiensis</i> SGE in French Polynesian residents.

<p>The figure presents the individual IgG responses (ΔOD) against <i>Ae</i>. <i>aegypti</i> (A) and <i>Ae</i>. <i>polynesiensis</i> (B) SGE in metropolitan France (n = 66) and in Tahiti and Moorea islands (French Polynesia, n = 47) residents. Each triangle or dot represents an individual sample and the horizontal bar indicates the median value. The dotted lines correspond to the positivity thresholds calculated from the cohort of metropolitan French residents, not exposed to these <i>Aedes</i> species (0.16 for <i>Ae</i>. <i>aegypti</i> and 0.13 for <i>Ae</i>. <i>polynesiensis</i>). The percentage of responders (positive samples) in the Tahiti-Moorea cohort is shown above the plot. The non-parametric Mann-Whitney test was used to compare groups.</p

Evolution of antibody responses to <i>Ae</i>. <i>aegypti</i> and <i>Ae</i>. <i>polynesiensis</i> SGE in military personnel.

<p>The figure presents individual IgG responses (ΔOD) to <i>Ae</i>. <i>aegypti</i> (A) and <i>Ae</i>. <i>polynesiensis</i> (B) SGE of French military personnel (n = 13) assigned in Tahiti island (French Polynesia). Blood from the same individual was sampled two weeks after arrival and one year later (paired data). Each triangle or dot represents an individual serum and the horizontal bar indicates the median value. The dotted lines correspond to the positivity thresholds calculated from the cohort of metropolitan French residents (0.16 and 0.13 for <i>Ae</i>. <i>aegypti</i> and <i>Ae</i>. <i>polynesiensis</i>, respectively). Percentages of responders are shown above each plot. The non-parametric Wilcoxon test was used to compare the paired groups.</p

Antibody responses to <i>Ae</i>. <i>aegypti</i> and <i>Ae</i>. <i>polynesiensis</i> SGE from cohorts from different contexts of exposure.

<p>The figure presents the individual IgG responses (ΔOD) against <i>Ae</i>. <i>aegypti</i> (A) and <i>Ae</i>. <i>polynesiensis</i> (B) SGE of residents from Martinique (n = 46), New-Caledonia (n = 19), Bolivia (n = 30) and Reunion (n = 33). The horizontal bars indicate the median value in each group and the dotted lines correspond to the positivity thresholds calculated from the cohort of metropolitan French residents. The percentage of responders in each cohort is shown above the plot. Residents from Martinique, New Caledonia and Bolivia, are typically exposed to <i>Ae</i>. <i>aegypti</i> bites. In Reunion island, <i>Ae</i>. <i>albopictus</i> is the main <i>Aedes</i> species while <i>Ae</i>. <i>aegypti</i> is cryptic. <i>Ae</i>. <i>polynesiensis</i> is not present in these islands or countries.</p

<i>Aedes aegypti</i> from the Pacific region infected with ZIKV.

<p><b>(A) Infection rate, (B) dissemination rate, (C) transmission rate and (D) transmission efficiency at 6, 9, 14 and 21 days post-infection (dpi).</b> Error bars represent 95% confidence intervals. Numbers of mosquitoes tested are indicated above each bar plot. Significant differences are indicated by asterisks (*<i>p</i> < 0.05; **<i>p</i> < 0.01; *** <i>p</i> < 0.001). NT indicates that females were not tested for this analysis point.</p

<i>Aedes aegypti</i> and <i>Aedes polynesiensis</i> populations sampled in the Pacific region, 2016.

<p>When available, data regarding the number of individuals sampled are provided in brackets.</p

Pacific map locating <i>Ae</i>. <i>aegypti</i> and <i>Ae</i>. <i>polynesiensis</i> sampling sites.

<p><i>Ae</i>. <i>aegypti</i> sampling sites are represented by the red stars and <i>Ae</i>. <i>polynesiensis</i> sampling sites by the dark star in a white dot. This map was generated using map files provided by ESRI in its ArcMap package.</p

<i>Aedes polynesiensis</i> from the Pacific region infected with ZIKV.

<p><b>(A) Infection rate, (B) dissemination rate, (C) transmission rate and (D) transmission efficiency at 6, 9, 14 and 21 days post-infection (dpi).</b> Error bars represent 95% confidence intervals. Numbers of mosquitoes tested are indicated above each bar plot. Significant differences are indicated by asterisks (*<i>p</i> < 0.05; **<i>p</i> < 0.01; *** <i>p</i> < 0.001).</p

Differentially expressed salivary gland proteins between the susceptible Kis and resistant AceRKis strains.

<p>Protein identification was performed using MALDI-TOF MS or nanoLC ESI MS/MS (underlined spot#) analysis. Insecta entries of Swiss-Prot and TrEMBL databases were searched by using the MASCOT algorithm, or the Proteome Discoverer software for nanoLC ESI MS/MS spectra. All spots displayed a power >0.9. Spot# refers to the SameSpots analysis spot number (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103816#pone-0103816-g001" target="_blank">Figure 1A</a>); ^SP indicates the presence of a signal peptide for secretion as predicted by SignalP 4.0 (<i>^SP°</i>: SP in the N-terminal homolog Q5TV62_ANOGA); Fold: fold difference in expression levels between the two strains; Accession: accession number in UniProtKB/Swiss-Prot or UniProtKB/TrEMBL databases (_ANOGA); pI: isoelectric point; Cover: indicates the amino acid coverage (%); *Mascot Score is provided for MALDI-TOF protein identification; for nanoLC ESI MS/MS based identification the peptide sequences and the number of peptides are provided as supporting information (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103816#pone.0103816.s001" target="_blank">Table S1</a>). Translation IF: Translation Initiation Factor.</p

Differential expression of the salivary proteins between the susceptible Kis and resistant AceRKis SGE extracts.

<p>Differences in protein expression are represented as a function of both expression ratio (resistant/susceptible) and significance ratio (q-value). Vertical dotted lines indicate the 1.5-fold difference in expression level in either direction (x1.5 for a higher expression in the resistant strain and/1.5 for a lower expression in the resistant strain). The horizontal dotted line indicates a q-value  = 0.05 (or 1/q  = 20). Saglin and TRIO proteins display expression level differences above 3 and highly significant q-values.</p

Phylogenetic tree obtained with a Bayesian inference of concatenated CO1 and ND4 sequence data.

<p>Numbers in parentheses indicate the number of samples belonging to this haplotype. For the Australian sample, only the CO1 sequence was available. Rooting was inferred from DNA sequences of <i>Anopheles pullus</i> and <i>Culex quinquefasciatus</i> but were not represented for clarity</p