Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation-3

Ase was -1.49 (n = 9); average band volume ratio from western blot analysis is -1.47 (n = 3). Variations in sample loading revealed by the actin signal is taken into account for the calculation of the average band volume ratio.<p><b>Copyright information:</b></p><p>Taken from "Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation"</p><p>http://www.proteomesci.com/content/6/1/14</p><p>Proteome Science 2008;6():14-14.</p><p>Published online 22 May 2008</p><p>PMCID:PMC2453108.</p><p></p

Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation-4

<p><b>Copyright information:</b></p><p>Taken from "Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation"</p><p>http://www.proteomesci.com/content/6/1/14</p><p>Proteome Science 2008;6():14-14.</p><p>Published online 22 May 2008</p><p>PMCID:PMC2453108.</p><p></p

Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation-0

<p><b>Copyright information:</b></p><p>Taken from "Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation"</p><p>http://www.proteomesci.com/content/6/1/14</p><p>Proteome Science 2008;6():14-14.</p><p>Published online 22 May 2008</p><p>PMCID:PMC2453108.</p><p></p

Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation-2

band volume ratio from western blot analysis was -1.28 (n = 3). Average ratio from 2D-DIGE analysis of the spot containing alpha-SNAP was -1.37 (n = 9); average band volume ratio from western blot analysis was -1.15 (n = 3). For all western blots, the variations in sample loading revealed by the actin signal is taken into account for the calculation of the average band volume ratio.<p><b>Copyright information:</b></p><p>Taken from "Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation"</p><p>http://www.proteomesci.com/content/6/1/14</p><p>Proteome Science 2008;6():14-14.</p><p>Published online 22 May 2008</p><p>PMCID:PMC2453108.</p><p></p

Geographic origin of the samples used for genomic studies of <i>PFI1785w</i>.

<p>Geographic origin of the samples used for genomic studies of <i>PFI1785w</i>.</p

Skyline output and calibration range for GHTVDSELTTEDPVIQKK.

<p>Retention time is displayed on x axis and intensity for each ion or fragment is displayed on y axis. Each parent ion and fragment is shown in a different colour. AUC corresponds to the integration of signal under the curve (A). Regression line is obtained using AUC from each concentration point.</p

Secondary structure of PFI1785w predicted using Phyre 2 and predictive 3D structure of amino-acid 224–360.

<p>Secondary structure of PFI1785w predicted using Phyre 2 and predictive 3D structure of amino-acid 224–360.</p

Peptide concentration measured within calibration range of each peptide.

<p>Peptide concentrations calculated by linear regression for each studied protein, y axis presents peptide concentration. Each dot represents one sample, and reference strains are displayed separately on the right of each plot (CSA+: CSA adhesion selected parasite strain, CSA-: non selected parasite strain). PAM samples and CSA+ parasite strains display a placental malaria like binding phenotype (in red), and UM samples and CSA- parasite strain display a uncomplicated malaria phenotype (in blue). PF14_0018, PFA_0410w, PFB0115w and PFI1785w are preferentially found in PAM like samples.</p

Linear regression line obtained with Skyline integration of raw files.

<p>Linear regression line obtained with Skyline integration of raw files.</p

CD8+ T-cell clones raised against contaminated PI_A7-21 peptide are IGRP_206-214-specific.

<p>(A) Schematic of the T-cell expansion and cloning technique. (B) Sorting of epitope-reactive CD8+ T cells based on CD107a/b upregulation after <i>in vitro</i> peptide recall. Left plot shows negative control (no stimulation); right plot shows CD107a/b staining after stimulation with contaminated PI_A7-21 peptide. (C) Representative TMr staining of one of the 3 T-cell clones generated. Staining for NRP-V7, PI_B15-23 and negative control TUM K^d TMr is shown from left to right.</p