Comparison of adipokines and their receptors (AdipoR1, AdipoR2 and Ob-R), COXs, F₂-isoprostanes, prostaglandin F_2α, α-SMA and aromatase in breast tissues adjacent to the tumor and tumor tissues of breast cancer patients based on a score calculated from the staining intensity and percent of area immunostained tissues.

<p>Results are the mean ± SEM; ns = not significant; Adipo R1/R2 = adiponectin receptor; Ob-R = leptin receptor; COX = cyclooxygenase; PGF_2α = prostaglandin F_2α; α-SMA = α-Smooth Muscle Actin</p><p>Comparison of adipokines and their receptors (AdipoR1, AdipoR2 and Ob-R), COXs, F₂-isoprostanes, prostaglandin F_2α, α-SMA and aromatase in breast tissues adjacent to the tumor and tumor tissues of breast cancer patients based on a score calculated from the staining intensity and percent of area immunostained tissues.</p

F₂-isoprostane and PGF_2α immunostaning in adjacent breast tissue to tumor or breast tumor tissue.

<p>Adjacent breast tissue to tumor or breast tumor tissue labeled by indirect immunofluorescence for A/ F₂-isoprostane and B/ PGF_2α (green) with DAPI as nuclear counterstain (blue).</p

Comparison of proliferation markers (Ki67 and β-Catenin) in breast tissues adjacent to the tumor and tumor tissues of breast cancer patients based on immunohistochemistry staining.

<p>C: cytoplasma; N = nucleus; n = number of samples</p><p>* % area stained.</p><p>Comparison of proliferation markers (Ki67 and β-Catenin) in breast tissues adjacent to the tumor and tumor tissues of breast cancer patients based on immunohistochemistry staining.</p

COX-1 and COX-2 immunostaining in adjacent breast tissue to tumor or breast tumor tissue.

<p>Adjacent breast tissue to tumor or breast tumor tissue labeled by indirect immunofluorescence for A/ COX-1 (red) and B/ COX-2 (green) with DAPI as nuclear counterstain (blue).</p

Adiponectin and Adiponectin receptors immunostaining in adjacent breast tissue to tumor or breast tumor tissue.

<p>Adjacent breast tissue to tumor or breast tumor tissue labeled by indirect immunofluorescence for A/ Adiponectin, and B/ Adiponectin receptor 1 and C/ Adiponectin receptor 2 (red) with DAPI as nuclear counterstain (blue).</p

β-Catenin and Ki67 immunostaining in adjacent breast tissue to tumor or breast tumor tissue.

<p>Adjacent breast tissue to tumor or breast tumor tissue labeled by indirect immunofluorescence for A/ β-catenin and B/ Ki67 (green) with DAPI as nuclear counterstain (blue).</p

Correlation between staining score values of COX-2 and adipokines and their receptors, aromatase, COX-1 and PGF_2α in healthy and tumor tissues as performed by Pearson test.

<p>COX = cyclooxygenase; Adipo R1/R2 = adiponectin receptor; PGF_2α = prostaglandin F_2α</p><p>Correlation between staining score values of COX-2 and adipokines and their receptors, aromatase, COX-1 and PGF_2α in healthy and tumor tissues as performed by Pearson test.</p

Comparison of adipokines and their receptors (AdipoR1, AdipoR2 and Ob-R), COXs, aromatase, F₂-isoprostanes, prostaglandin F_2α, α-SMA and aromatase in breast tissues adjacent to the tumor and tumor tissues of breast cancer patients on the basis of percent of staining area.

<p>Results are the mean ± SEM; ns: not significant. AU = Arbitrary Unit; Adipo R1/R2 = adiponectin receptor; Ob-R = leptin receptor; COX = cyclooxygenase; PGF_2α = prostaglandin F_2α; α-SMA = α-Smooth Muscle Actin</p><p>Comparison of adipokines and their receptors (AdipoR1, AdipoR2 and Ob-R), COXs, aromatase, F₂-isoprostanes, prostaglandin F_2α, α-SMA and aromatase in breast tissues adjacent to the tumor and tumor tissues of breast cancer patients on the basis of percent of staining area.</p

Comparison of adipokines and their receptors (AdipoR1, AdipoR2 and Ob-R), COXs, F₂-isoprostanes and prostaglandin F_2α, α-SMA and aromatase in the breast tissues adjacent to the tumor and tumor tissues of breast cancer patients on the basis of staining intensity.

<p>Results are the mean ± SEM; ns: not significant. AU = Arbitrary Unit; Adipo R1/R2 = adiponectin receptor; Ob-R = leptin receptor; COX = cyclooxygenase; PGF_2α = prostaglandin F_2α; α-SMA = α-Smooth Muscle Actin</p><p>Comparison of adipokines and their receptors (AdipoR1, AdipoR2 and Ob-R), COXs, F₂-isoprostanes and prostaglandin F_2α, α-SMA and aromatase in the breast tissues adjacent to the tumor and tumor tissues of breast cancer patients on the basis of staining intensity.</p

Additional file 1: of Physical basis of the ‘magnification rule’ for standardized Immunohistochemical scoring of HER2 in breast and gastric cancer

Figure S1. Example photomicrographs of HER2-IHC. Images depict scoring categories 1+, 2+ and 3+ at magnifications reflecting different microscope objectives (2.5× - 63×. Inserts: Magnified details, 4× additional magnification). Note that the linear DAB-precipitates in categories 1+ and 2+ are not perceivable at low power magnification (2.5×, 5×). (TIFF 47314 kb