Additional file 2 of Effects of rearing mode on gastro-intestinal microbiota and development, immunocompetence, sanitary status and growth performance of lambs from birth to two months of age

Supplementary Material

Additional file 1 of Effects of rearing mode on gastro-intestinal microbiota and development, immunocompetence, sanitary status and growth performance of lambs from birth to two months of age

Supplementary Material

Additional file 4 of Effects of rearing mode on gastro-intestinal microbiota and development, immunocompetence, sanitary status and growth performance of lambs from birth to two months of age

Supplementary Material

Additional file 3 of Effects of rearing mode on gastro-intestinal microbiota and development, immunocompetence, sanitary status and growth performance of lambs from birth to two months of age

Supplementary Material

Survival of EHEC co-incubated with <i>L</i>. <i>reuteri</i> strains in RF samples supplemented or not with glycerol.

<p>The strain FCH6 Rif^R was co-inoculated with ≈ 10⁷ CFU/mL of <i>L</i>. <i>reuteri</i> LB1-7 (HPA producer) or 100–23 (negative control) in RF samples under anaerobiosis for 24 hours. The strain FCH6 Rif^R inoculated alone in RF samples was used as control. RF samples were supplemented or not with glycerol at different concentrations. Bars represent the SEM of three independent experiments. Asterisks indicate statistical significance (***: P<0.001).</p

Growth or survival of EHEC in rectum contents after incubation in RF.

<p>The strain FCH6 Rif^R was first incubated in filter-sterilized RF (FS-RF) samples alone or inoculated with ≈ 10⁷ CFU/mL of <i>L</i>. <i>reuteri</i> LB1-7 supplemented with 80 mM glycerol under anaerobiosis for 24 hours. The bacterial pellet was then inoculated into Rec samples and incubated under anaerobiosis. RF = 0 represents inoculation of EHEC in FS-RF samples; Rec t = 0 corresponds to RF t = 24h i.e. number of EHEC surviving the incubation in FS-RF during 24 hours; Rec t = 6h and Rec t = 24h correspond to EHEC survival in Rec samples after 6 and 24 hours of incubation respectively. Bars represent the SEM of three independent experiments. Effect of <i>L</i>. <i>reuteri</i> + Glyc80 is significant *, P<0.05; ***, P<0.001.</p

EHEC counts and HPA, 1,3-PD and glycerol quantification in RF-Glyc80.

<p>The strain FCH6 Rif^R (≈ 10⁴ CFU/mL) was co-incubated with <i>L</i>. <i>reuteri</i> LB1-7 (≈ 10⁷ CFU/mL) in RF samples supplemented with 80 mM glycerol under anaerobiosis. At each time point the strain FCH6 Rif^R was enumerated and accumulation of HPA and 1,3-PD, and disappearance of glycerol were monitored. Bars represent the SEM of three independent experiments. Gly: glycerol.</p

Concentration of glycerol and microbial metabolites.

<p>Concentration of glycerol and microbial metabolites.</p

Kinetics of EHEC growth or disappearance and HPA production in LB broth.

<p>(A) The strain FCH6 Rif^R (≈ 10⁴ CFU/mL) was co-incubated with <i>L</i>. <i>reuteri</i> LB1-7 (≈ 10⁷ CFU/mL) in LB broth supplemented or not with different concentration of glycerol. The strain FCH6 Rif^R was then enumerated after 24 hours of incubation under anaerobiosis. Bacterial growth curves are expressed as a single representation of three independent experiments. (B) The strain FCH6 Rif^R was co-incubated with <i>L</i>. <i>reuteri</i> in LB broth supplemented with 10 mM glycerol under anaerobiosis. At each time point the strain FCH6 Rif^R was enumerated and accumulation of HPA was quantified. The bacterial growth curve is expressed as a single representation of three independent experiments. Bars represent the SEM of three independent experiments.</p