LXR protects from hepatic damage induced by an oleic-rich diet.

<p>(A) <i>Tnf</i>α, <i>Ccl2</i>, <i>F4/80</i>, <i>Cd68</i> and <i>Il1β</i> mRNA quantification assayed by qPCR. (B) Plasma activity of ALT and AST. (C) Plasma cholesterol and lathosterol levels analyzed by gas chromatography. Data are the mean±SEM of values measured in LXR+/+ and LXR-/- mice fed the REF or the OLIV diet. ^a Significant genotype effect. ^b Significant difference versus REF diet (n = 6 mice per group).</p

Effects of an oleic acid-rich diet on hepatic fatty acid profile in LXR+/+ and LXR-/- mice.

<p>Effects of an oleic acid-rich diet on hepatic fatty acid profile in LXR+/+ and LXR-/- mice.</p

High oleic acid diet modulates hepatic gene expression.

<p>Hepatic gene expression of 142 genes related to lipid metabolism, nuclear receptor signaling and inflammation were quantified by qPCR from liver of LXR+/+ and LXR-/- mice fed the REF or the OLIV diet. Data are presented as a heatmap associated with a hierarchical classification.</p

LXR mediate the induction of lipogenesis induced by an oleic acid-rich diet.

<p>(A) Hepatic <i>Acly</i>, <i>Acaca</i>, <i>Acacb</i>, <i>Fasn</i>, <i>Elovl6</i>, <i>Scd1</i> mRNA levels quantified by qPCR. (B) Cytoplasmic protein expression levels of P-ACLY, ACLY, ACC, ELOVL6, SCD1, FASN AND β-ACTIN assayed by Western Blotting. (C) <i>Fads1</i>, <i>Fads2</i>, <i>Elovl5</i>, <i>Gpat</i>, <i>Pnpla3</i> and <i>Lpk</i> mRNA quantification assayed by qPCR. (D) <i>Srebp-1c</i> and <i>Chrebp</i> mRNA quantification assayed by qPCR. (E) Cytoplasmic and nuclear expression levels of LXR, SREBP-1c and ChREBP assayed by Western Blotting. Data are the mean±SEM of values measured in LXR+/+ and LXR-/- mice fed REF or OLIV diet. ^a Significant genotype effect. ^b Significant difference versus REF diet (n = 6 mice per group).</p

High oleic acid diet induces hepatic steatosis in LXR+/+ but not in LXR-/- mice.

<p>(A) Representative Oil Red O-stained frozen sections of liver from mice of both genotypes fed the REF or the OLIV diet (Scale bars: 100 μm). Neutral lipids appear in red. (B) Liver triglycerides, cholesterol and cholesterol esters measured by gas chromatography. Data are the mean±SEM of values measured in LXR+/+ and LXR-/- mice fed the REF or the OLIV diet. ^a Significant genotype effect. ^b Significant difference versus REF diet (n = 6 mice per group).</p