Validation of twelve microarray differentially expressed genes by quantitative PCR.

<p>Fold change value is expressed as the expression ratio of Basque (B, n = 20) to Large White (LW, n = 20) samples when genes are highly expressed in Basque pigs and as the negative expression ratio of LW to B samples when genes are highly expressed in LW pigs. Statistical significances are reported below the plot as Benjamini and Hochberg adjusted P value for microarray data and as Student t-test P value for qPCR data (bold case). <i>ADAMTS8</i>, ADAM metallopeptidase with thrombospondin type 1 motif, 8; <i>ANKRD1</i>, ankyrin repeat domain 1; <i>FABP3</i>, Fatty acid-binding protein, heart; <i>FHL3</i>, Four and a half LIM domains 3; <i>FOS</i>, FBJ murine osteosarcoma viral oncogene homolog; <i>GLOD4</i>, glyoxalase domain containing 4; <i>HHATL</i>, hedgehog acyltransferase-like; <i>IGF1</i>, insulin-like growth factor 1; <i>LIPE</i>, lipase, hormone-sensitive; <i>PPARD</i>, peroxisome proliferator-activated receptor delta; <i>SPARC</i>, secreted protein acidic and rich in cysteine; <i>ZNF24</i>, zinc finger protein 24.</p

Differences between Basque (B, n = 20) and Large White (LW, n = 20) breeds for growth, body composition, and Longissimus muscle characteristics.

a<p>Residual Standard Deviation.</p>b<p>P value of breed effect.</p>c<p>Score between 0 to 10.</p>d<p>A significant effect of housing system was found (A: 4.0 and C: 4.4, P = 1.8E-02).</p

Functional annotation clustering for genes highly expressed in the Longissimus muscle of Large White (LW, n = 20) pigs.

a<p>Gene ontology identification number.</p>b<p>Name of the ontology.</p>c<p>n_G, number of genes in the category.</p>d<p>modified Fisher's exact test P value.</p

Functional annotation clustering for genes highly expressed in the Longissimus muscle of Basque (B, n = 20) pigs.

a<p>Gene ontology identification number.</p>b<p>Name of the ontology.</p>c<p>n_G, number of genes in the category.</p>d<p>Modified Fisher's exact test P value.</p

Microarray Biological Process (GO Slim) classification.

<p>Each Biological Process category is represented by a bar. The height of the bar represents the percentage of genes observed in the category. The number of genes per category is indicated upon the bars text.</p

Relevant biological processes significantly enriched in the two lists of differentially expressed genes.

a<p>Gene ontology identification number.</p>b<p>n_G = number of genes in the category.</p>c<p>Modified Fisher's exact test P value.</p

Boletín de Segovia: Número 32 - 1898 marzo 16

Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200