Distribution of the fold-changes of all proteins quantified (185) in both biological replicates of the LD growth cycle using the post-digest ICPL method.

<p>Distribution of the fold-changes of all proteins quantified (185) in both biological replicates of the LD growth cycle using the post-digest ICPL method.</p

Growth curve of <i>A</i>. <i>platensis</i> under a 12-hour LD cycle.

<p>Growth curve of <i>A</i>. <i>platensis</i> under a 12-hour LD cycle.</p

Figure 2

<p>IMAC-Copper chromatogram (A). 4-20% SDS PAGE (B). Venn diagram showing non-redundant proteins between the two fractions (C). The twenty highest emPAI values in both the flowthrough (inner circle) and elution (outer circle) fractions (D). Histogram representing the emPAI values of the 6 most abundant proteins found to be common between the flowthrough and eluate (E).</p

Cartoon depicting proteins associated with photosynthesis that are differentially regulated under the LD cycle.

<p>Each protein of the diagram is colored based on increased (red) or decreased abundance (blue) relative to the dark growth phase. Proteins colored in grey were not quantified.</p

Venn diagram showing non-redundant proteins identified using: IEF, SDS-PAGE, 2D-LC, and 3D-LC MS/MS (IMAC-Cu²⁺).

<p>Venn diagram showing non-redundant proteins identified using: IEF, SDS-PAGE, 2D-LC, and 3D-LC MS/MS (IMAC-Cu²⁺).</p

The molecular response of <i>Arthrospira</i> sp. PCC 8005 to nitrogen limitation: the glycolysis, endogenous-N and arginine metabolism.

<p>Molecular pathways were predicted according to the Genoscope and KEGG databases. The squares correspond to protein abundance (nitrogen limitation versus control) and the circles correspond to mRNA expression (nitrogen limitation versus control). The data presented here were taken from three biologically independent replicates.</p

The molecular response of <i>Arthrospira</i> sp. PCC 8005 to nitrogen limitation: the pentose phosphate pathway (OPP), protein and phycobilisome degradation, glycogen metabolism and citric acid cycle.

<p>Molecular pathways were predicted according to the Genoscope and KEGG databases. The squares correspond to protein abundance (nitrogen limitation versus control) and the circles correspond to mRNA expression (nitrogen limitation versus control). The data presented here were taken from four biologically independent replicates.</p

The molecular response of <i>Arthrospira</i> sp. PCC 8005 to nitrogen limitation: the glycolysis, endogenous-N and arginine metabolism.

<p>Molecular pathways were predicted according to the Genoscope and KEGG databases. The squares correspond to protein abundance (nitrogen limitation versus control) and the circles correspond to mRNA expression (nitrogen limitation versus control). The data presented here were taken from three biologically independent replicates.</p

Dry weight (g L^-1) and composition (%) of control and N-stressed <i>Arthrospira</i> sp. biomass at the end of the experiment (day 10).

<p>FAME, fatty acid methyl esters. Statistical analyses were performed using Sigma-plot 11 (Systat Software, Inc.). Before evaluating the results with one-way analysis of variance (ANOVA), normality of the data was determined with the Shapiro-Wilk normality test. To analyze the pairwise differences, a Tukey’s post-hoc test was used. The significance level of statistical analyses (<i>p-</i>value) was set at 5%.</p><p>^a,b Different letters indicate statistical difference.</p><p>Dry weight (g L^-1) and composition (%) of control and N-stressed <i>Arthrospira</i> sp. biomass at the end of the experiment (day 10).</p

Changes of biomass density evaluated by measuring the optical density at 750 nm and NO₃-N concentration (mg L^-1) in the culture medium of the control (○) and N-limited <i>Arthrospira</i> sp. PCC 8005 (●).

<p>Changes of biomass density evaluated by measuring the optical density at 750 nm and NO₃-N concentration (mg L^-1) in the culture medium of the control (○) and N-limited <i>Arthrospira</i> sp. PCC 8005 (●).</p