178 research outputs found
Genetic evaluation considering phenotypic data and limited molecular information using a novel equivalent model: Case study using effect of the mh locus on milk production in the dual-purpose Belgian Blue breed
The introduction of molecular information into genetic evaluation systems is currently under
research. Based on an equivalent method, we developed from existing theory a new
alternative strategy for the prediction of gene effects and especially their smooth integration
into genetic evaluations. Underlying hypothesis were based on the idea that knowledge of
genotypes will not affect overall additive genetic variance but only change expected values of
genetic effects for animals with known genotypes. However, all animals could not be
genotyped. Thus, the developed equations were modified to allow the integration of the
known genotype for a portion of the population. This strategy was tested for the mh locus
(responsible for the double-muscling phenotype) in dual-purpose Belgian Blue cattle. The
genotype was determined for 123 bulls and 1940 cows (+/+ 19.5, mh/+ 39.3 and mh/mh
41.2%). These animals had 11,150 daughters with test-day (TD) records. The genotypes were
incorporated into a modified genetic evaluation based on the current routine multi-trait multibreed
test-day model used in the Walloon Region (Belgium). Data used included 12,829,309
TD records for 689,057 dairy cows in production. The pedigree file contained 1,606,024
animals (cows with TD records and ancestors). Computation of the modified mixed model
equations was done solving iteratively two systems of equations, one for the polygenic effects
and one for the gene effect until the relative differences in the gene solutions were below 10-5.
A linear extrapolation was also used to speed up the convergence of gene effects. As
expected, the mh locus exerts negative effects on milk production traits. For the first three
lactations, the average estimated allelic substitution effects were -158.7 kg milk, -8.93 kg fat
and -5.64 kg protein per lactation (305 days)
Molecular characterization of the bovine GHRL gene
peer reviewedaudience: researcherBovine ghrelin, a 27 amino acid pepticle, has been identified in oxyntic glands of the abomasum. It is an endogenous ligand for growth hormone secretagogue receptor and stimulates food intake and growth hormone secretion. The bovine GHRL gene was completely sequenced and consists of five exons and four introns. Like mouse and human GHRL genes, we found that the bovine GHRL gene also contains a first non-coding exon of 21 bp. The bovine GHRL gene codes for 116 amino acid pepticle named preproghrelin which contains the ghrelin pepticle and another pepticle similar to obestatin. Sequence analysis revealed eight polymorphisms, which are located in the non-coding sequence of the gene
Overview of possibilities and challenges of the use of infrared spectrometry in cattle breeding
peer reviewedNear or mid-infrared (NIR or MIR) spectrometry is a versatile and cost-efficient technology used in cattle production to trace the chemical composition of gases, liquids and solid matters. Recent research showed the potential of MIR spectrometry in milk to predict many different milk components but also status and well-being of the cows, quality of their products, their efficiency and their environmental impact. Under changing socio-economic circumstances, novels traits could help to select for enlarged breeding objectives. But the following challenges need to be overcome: (1) access to and harmonization of MIR data; (2) availability of reference values representing the variability to be described, also highlighting the importance of international collaborations; (3) difficulties to obtain, but also to transfer prediction equations between instruments; (4) modeling of the massive longitudinal data generated; (5) estimation of parameters to assess phenotypic and genetic variability and links with other traits leading to the; (6) assessment of the position of novel traits in breeding objectives. Recent research reported how to address these issues for traits close to routine use including fatty acids and methane. Expected future developments include direct use of MIR data and multivariate modeling of novel traits. Similarly, genomic prediction for novel traits, which are limited by the availability of phenotyped reference populations, will also benefit from the use of correlated, MIR predicted, traits. Currently, MIR instruments can only be used in the frame of milk recording and not on-farm. But recent research showed that NIR is closing the gap thereby allowing advances in precise on-farm phenotyping and giving new opportunities for breeding, but also management. Possibilities for the use of infrared technologies for other trait groups such as meat composition and quality should allow cross-fostering of developments
Mid-infrared predictions of cheese yield from bovine milk
audience: researcher, professiona
Potential use of mid-infrared spectrometry to predict cheese yield from milk and to study its genetic variability
peer reviewedFournir une indication rapide, fiable et bon marché du rendement fromager pour un lait donné, sans devoir appliquer des formules (empiriques ou théoriques) à partir des concentrations préalablement déterminées pour différents constituants du lait, serait un outil utile et économiquement intéressant tant pour les éleveurs que pour l’industrie laitière. En vue d’étudier la variabilité génétique du rendement fromager à l’échelle du cheptel bovin wallon, des méthodes chimiométriques ont été utilisées afin de développer des équations de prédictions basées sur des spectres moyen infrarouge (MIR) pour les rendements fromagers déterminés en laboratoire et exprimés en frais (RdFF) ou en sec (RdFS). Ceux-ci ont été déterminés sur 258 échantillons de lait analysés en spectrométrie MIR. Les équations de prédiction à partir du spectre MIR du lait ont été développées en utilisant la régression des moindres carrés partiels (PLS) avec une validation croisée interne appliquée sur la dérivée première des spectres MIR. Les coefficients de détermination de validation croisée (R²cv) des équations étaient de 0,81 pour les prédictions du RdFF et de 0,82 pour les celles du RdFS. Les rapports des performances sur les variabilités (RPD) étaient égaux à 2,3. Ces résultats peuvent permettre d’envisager une bonne utilité pratique pour leur prédiction respective, notamment dans le cadre de recherches génétiques. Ces équations ont été appliquées sur la base de données spectrales générée dans le cadre du contrôle laitier wallon. Les composantes de la variance ont été estimées séparément pour le RdFF et le RdFS basées sur un modèle animal « contrôles élémentaires » utilisant des régressions aléatoires. Le jeu de données utilisé comportait 51 537 prédictions pour 7 870 vaches primipares Holstein. Les héritabilités journalières moyennes variaient entre 0,31 (au 5ème jour de lactation (JDL)) et 0,59 (au 279ème JDL) pour le RdFF et entre 0,31 (au 5ème JDL) et 0,57 (au 299ème JDL) pour le RdFS. Ces héritabilités journalières modérées à élevées ont indiqué le potentiel de sélection génétique pour ces deux caractères.ProFARMilk, BlueSe
Estimation of Myostatin gene effects on production traits and fatty acid contents in bovine milk
peer reviewedThe aim of this study was to estimate the genetic parameters of milk, fat, and protein yields, saturated (SFA) and monounsaturated fatty acid (MUFA) contents in bovine milk and to estimate the Myostatin (mh) gene effect on these traits. For this purpose, 51,614 test-day records (24,124, 16,145, and 11,345 for first, second and third lactation, respectively) of 3,098 dual purpose Belgian Blue cows in 38 herds from the Walloon Region of Belgium were used. Because only 2,301 animals, including 1,082 cows with test-day records, were genotyped for mh, the gene content of non-genotyped animals was predicted from animals with a known genotype using the relationships with these animals. Variance components were estimated using Restricted Maximum Likelihood. A 3-lactations, 5-traits random regression test-day mixed model, based on the official Walloon genetic evaluation model for production traits, was used with an additional fixed regression on mh gene content to estimate allele substitution effects. Daily heritability estimates (average of 3 lactations) were 0.34 for SFA and 0.16 for MUFA and were higher than those for production traits (0.11, 0.10, and 0.09 for milk, fat, and protein yields, respectively). Allele substitution effects approximate standard-errors) for mh through the three lactations were-0.628 (+0.343),-0.024 (0.014) and -0.021 (+0.009) kg per day for milk, fat, and protein yields, respectively. Concerning SFA and MUFA contents in milk, the average allele substitution effects were -0.001 (+0.027) and 0.029 (+0.023) g/dl of milk. To conclude, results from this study showed that milk performance traits and milk fatty acid profile are influenced by mh genotypes
Genetic variability of lactoferrin content estimated by mid-infrared spectrometry in bovine milk
The effects of lactoferrin (LF) on the immune system
have already been shown by many studies. Unfortunately,
the current methods used to measure LF levels
in milk do not permit the study of the genetic variability
of lactoferrin or the performance of routine genetic evaluations.
The first aim of this research was to derive a
calibration equation permitting the prediction of LF in
milk by mid-infrared spectrometry (MIR). The calibration
with partial least squares on 69 samples showed
a ratio of standard error of cross-validation to standard
deviation equal to 1.98. Based on this value, the calibration
equation was used to establish an LF indicator
trait (predicted LF; pLF) on a large number of milk
samples (n = 7,690). A subsequent study of its variability
was conducted, which confirmed that stage of lactation
and lactation number influence the overall pLF
level. Small differences in mean pLF among 7 dairy
breeds were also observed. The pLF content of Jersey
milk was significantly higher than that in Holstein
milk. Therefore, the choice of breed could change the
expected LF level. Heritability estimated for pLF was
19.7%. The genetic and phenotypic correlations between
somatic cell score and pLF were 0.04 and 0.26,
respectively. As somatic cell score increases in presence
of mastitis, this observation seems to indicate that pLF,
or a function of observed pLF, compared with expected
LF might have potential as an indicator of mastitis.
The negative genetic correlation (−0.36) between milk
yield and pLF could indicate an undesirable effect of
selection for high milk production on the overall LF
level
They live under our streets: ant nests (Hymenoptera, Formicidae) in urban pavements
peer reviewedIn the context of global insect decline, the urbanisation process plays a key role. However, urban pavements, which are considered to be impervious to biodiversity, can harbour ground-nesting insects under certain conditions. Recent observations have revealed the presence of Formicidae nests under urban pavements. The aim of this work is to determine the species richness of Formicidae nesting under urban pavements in the Brussels-Capital Region (Belgium) and to characterise their nest environment and soil texture. Seven ant species were identified in 120 nesting sites: Lasius niger, Lasius brunneus, Lasius flavus, Lasius fuliginosus, Tetramorium caespitum, Tetramorium impurum and Myrmica rugulosa. Concrete slabs or natural stones with a sandy sub-layer are the main structures in which ants nest. In addition, nests were mainly found under modular pavements with degraded rigid joints. The results of this work highlight the capacity of urban structures to host part of ant biodiversity in cities
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