Tool post modification allows easy turret lathe cutting-tool alignment

Modified tool holder and tool post permit alignment of turret lathe cutting tools on the center of the spindle. The tool is aligned with the spindle by the holder which is kept in position by a hydraulic lock in feature of the tool post. The tool post is used on horizontal and vertical turret lathes and other engine lathes

Infant Social Interactions With Multiple Caregivers: The Importance of Ethnicity and Socioeconomic Status

Abstract Most studies of diverse populations of families within the United States have either focused predominantly on ethnicity or socioeconomic status (SES), and those that have examined both ethnicity and SES have noted difficulties in disentangling the effects of SES and ethnicity. In order to achieve a greater understanding of variation in infant experiences with parental and nonparental caregivers in differing socioeconomic and ethnic contexts, 41 infants from African American and 40 infants from European American families of lower and middle SES were observed for 12 hours each in and around their home environments. Ethnic differences were evident in the infants' overall experiences with caregivers, maternal availability, affection, caregiving, and stimulation by nonnuclear relatives; SES differences were identified for maternal and paternal holding, maternal carrying, and paternal caregiving. When caregiver availability was taken into account, variations in interactional and care experiences were predominantly predicted by ethnicity. These results underscore the need to study both ethnicity and socioeconomic variation rather than either one alone. Furthermore, the caregiving behaviors of African American mothers and fathers may be misrepresented when multiple SES contexts are not considered. Keywords family/child rearing, infant/child cultural psychology, socioeconomic Ethnicity and socioeconomic status (SES) are frequently viewed as important factors affecting caregiving and other interactive experiences of infants and children (e.

High Data Rate HF Communications for BFTN Using Advanced Waveform Techniques

NPS NRP Project PosterIn this feasibility study, we propose the application of advanced waveforms techniques (e.g. Orthogonal Frequency Division Multiplexing, OFDM) for high data rate HF communications within the Battle Force Tactical Network (BFTN). OFDM and other multicarrier-type modulations can potentially mitigate the multipath problem that HF is subjected to and potentially increase the limited data throughput of HF systems. While SATCOM provides reliable RF communications and data links, the Navy needs a viable alternative to SATCOM. Beyond line of sight (BLOS) HF communications (2-30 MHz band) can be improved using advanced digital waveform techniques that are used in wireless and cellular communications. It can be a complementary system or be the solution to SATCOM-degraded scenarios.Naval Information Warfighting Development Center (NIWDC)U.S. Fleet Forces Command (USFF)This research is supported by funding from the Naval Postgraduate School, Naval Research Program (PE 0605853N/2098). https://nps.edu/nrpChief of Naval Operations (CNO)Approved for public release. Distribution is unlimited.

High Data Rate HF Communications for BFTN Using Advanced Waveform Techniques

NPS NRP Executive SummaryIn this feasibility study, we propose the application of advanced waveforms techniques (e.g. Orthogonal Frequency Division Multiplexing, OFDM) for high data rate HF communications within the Battle Force Tactical Network (BFTN). OFDM and other multicarrier-type modulations can potentially mitigate the multipath problem that HF is subjected to and potentially increase the limited data throughput of HF systems. While SATCOM provides reliable RF communications and data links, the Navy needs a viable alternative to SATCOM. Beyond line of sight (BLOS) HF communications (2-30 MHz band) can be improved using advanced digital waveform techniques that are used in wireless and cellular communications. It can be a complementary system or be the solution to SATCOM-degraded scenarios.Naval Information Warfighting Development Center (NIWDC)U.S. Fleet Forces Command (USFF)This research is supported by funding from the Naval Postgraduate School, Naval Research Program (PE 0605853N/2098). https://nps.edu/nrpChief of Naval Operations (CNO)Approved for public release. Distribution is unlimited.

Scholarship in Review 84(2)

Scholarship in Review was a magazine highlighting research and scholarly activities at Central Washington University, published by the Office of Graduate Studies and Research.https://digitalcommons.cwu.edu/scholarship_in_review/1000/thumbnail.jp

Paring Down HIV Env: Design and Crystal Structure of a Stabilized Inner Domain of HIV-1 gp120 Displaying a Major ADCC Target of the A32 Region

SummaryEvidence supports a role of antibody-dependent cellular cytotoxicity (ADCC) toward transitional epitopes in the first and second constant (C1-C2) regions of gp120 (A32-like epitopes) in preventing HIV-1 infection and in vaccine-induced protection. Here, we describe the first successful attempt at isolating the inner domain (ID) of gp120 as an independent molecule that encapsulates the A32-like region within a minimal structural unit of the HIV-1 Env. Through structure-based design, we developed ID2, which consists of the ID expressed independently of the outer domain and stabilized in the CD4-bound conformation by an inter-layer disulfide bond. ID2 expresses C1-C2 epitopes in the context of CD4-triggered full-length gp120 but without any known neutralizing epitope present. Thus, ID2 represents a novel probe for the analysis and/or selective induction of antibody responses to the A32 epitope region. We also present the crystal structure of ID2 complexed with mAb A32, which defines its epitope

Epithelial immunomodulation by aerosolized Toll-like receptor agonists prevents allergic inflammation in airway mucosa in mice

Allergic asthma is a chronic inflammatory respiratory disease associated with eosinophilic infiltration, increased mucus production, airway hyperresponsiveness, and airway remodeling. Epidemiologic data reveal that the prevalence of allergic sensitization and associated diseases has increased in the twentieth century. This has been hypothesized to be partly due to reduced contact with microbial organisms (the hygiene hypothesis) in industrialized society. Airway epithelial cells, once considered a static physical barrier between the body and the external world, are now widely recognized as immunologically active cells that can initiate, maintain, and restrain inflammatory responses, such as those that mediate allergic disease. Airway epithelial cells can sense allergens via expression of myriad Toll-like receptors (TLRs) and other pattern-recognition receptors. We sought to determine whether the innate immune response stimulated by a combination of Pam2CSK4 (“Pam2”, TLR2/6 ligand) and a class C oligodeoxynucleotide ODN362 (“ODN”, TLR9 ligand), when delivered together by aerosol (“Pam2ODN”), can modulate the allergic immune response to allergens. Treatment with Pam2ODN 7 days before sensitization to House Dust Mite (HDM) extract resulted in a strong reduction in eosinophilic and lymphocytic inflammation. This Pam2ODN immunomodulatory effect was also seen using Ovalbumin (OVA) and A. oryzae (Ao) mouse models. The immunomodulatory effect was observed as much as 30 days before sensitization to HDM, but ineffective just 2 days after sensitization, suggesting that Pam2ODN immunomodulation lowers the allergic responsiveness of the lung, and reduces the likelihood of inappropriate sensitization to aeroallergens. Furthermore, Pam2 and ODN cooperated synergistically suggesting that this treatment is superior to any single agonist in the setting of allergen immunotherapy

\u3ci\u3ePseudomonas syringae\u3c/i\u3e Hrp type III secretion system and effector proteins

Pseudomonas syringae is a member of an important group of Gram-negative bacterial pathogens of plants and animals that depend on a type III secretion system to inject virulence effector proteins into host cells. In P. syringae, hrpyhrc genes encode the Hrp (type III secretion) system, and avirulence (avr) and Hrpdependent outer protein (hop) genes encode effector proteins. The hrpyhrc genes of P. syringae pv syringae 61, P. syringae pv syringae B728a, and P. syringae pv tomato DC3000 are flanked by an exchangeable effector locus and a conserved effector locus in a tripartite mosaic Hrp pathogenicity island (Pai) that is linked to a tRNALeu gene found also in Pseudomonas aeruginosa but without linkage to Hrp system genes. Cosmid pHIR11 carries a portion of the strain 61 Hrp pathogenicity island that is sufficient to direct Escherichia coli and Pseudomonas fluorescens to inject HopPsyA into tobacco cells, thereby eliciting a hypersensitive response normally triggered only by plant pathogens. Large deletions in strain DC3000 revealed that the conserved effector locus is essential for pathogenicity but the exchangeable effector locus has only a minor role in growth in tomato. P. syringae secretes HopPsyA and AvrPto in culture in a Hrp-dependent manner at pH and temperature conditions associated with pathogenesis. AvrPto is also secreted by Yersinia enterocolitica. The secretion of AvrPto depends on the first 15 codons, which are also sufficient to direct the secretion of an Npt reporter from Y. enterocolitica, indicating that a universal targeting signal is recognized by the type III secretion systems of both plant and animal pathogens