Synthetic dye decolorization by three sources of fungal laccase

Decolorization of six synthetic dyes using three sources of fungal laccase with the origin of Aspergillus oryzae, Trametes versicolor, and Paraconiothyrium variabile was investigated. Among them, the enzyme from P. variabile was the most efficient which decolorized bromophenol blue (100%), commassie brilliant blue (91%), panseu-S (56%), Rimazol brilliant blue R (RBBR; 47%), Congo red (18.5%), and methylene blue (21.3%) after 3 h incubation in presence of hydroxybenzotriazole (HBT; 5 mM) as the laccase mediator. It was also observed that decolorization efficiency of all dyes was enhanced by increasing of HBT concentration from 0.1 mM to 5 mM. Laccase from A. oryzae was able to remove 53% of methylene blue and 26% of RBBR after 30 min incubation in absence of HBT, but the enzyme could not efficiently decolorize other dyes even in presence of 5 mM of HBT. In the case of laccase from T. versicolor, only RBBR was decolorized (93%) in absence of HBT after 3 h incubation. © 2012 Forootanfar et al.; licensee BioMed Central Ltd

Insights into laccase producing organisms, fermentation states, purification strategies, and biotechnological applications

Laccases are phenol oxidases belonging to the superfamily of multicopper oxidases and are found in bacteria, fungi, lichens, higher plants, and insects. Over the past few decades, laccases and laccase mediator systems (LMS) have found uses in a wide range of technological applications such as textile dye decolorization, industrial wastewater detoxification, pulp bleaching, chemical synthesis, and development of miniaturized biosensors. This has encouraged numerous studies to find and purify laccases with exploitable characteristics. The main aim of the present review is to summarize the rich literature data gained in recent years from the studies on laccases, focusing on the organisms that produce them, the methods used for screening, laccase activity assays, purification strategies, and the application of laccases as eco-friendly biocatalysts. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 2015

Regenerative medicine and drug delivery: Progress via electrospun biomaterials

Worldwide research on electrospinning enabled it as a versatile technique for producing nanofibers with specified physio-chemical characteristics suitable for diverse biomedical applications. In the case of tissue engineering and regenerative medicine, the nanofiber scaffolds' characteristics are custom designed based on the cells and tissues specific needs. This fabrication technique is also innovated for the production of nanofibers with special micro-structure and secondary structure characteristics such as porous fibers, hollow structure, and core- sheath structure. This review attempts to critically and succinctly capture the vast number of developments reported in the literature over the past two decades. We then discuss their applications as scaffolds for induction of cells growth and differentiation or as architecture for being used as graft for tissue engineering. The special nanofibers designed for improving regeneration of several tissues including heart, bone, central nerve system, spinal cord, skin and ocular tissue are introduced. We also discuss the potential of the electrospinning in drug delivery applications, which is a critical factor for cell culture, tissue formation and wound healing applications. © 201

New strategies for safe cancer therapy using electrospun nanofibers: A short review

Electrospun nanofibers regarding their special features, including high drug loading capacity, high surface to volume area, flexibility, and ease of production and operation, are of great interest for being used in tissue engineering, and drug delivery approaches. In this context, several studies have been done for the production of biodegradable and biocompatible scaffolds containing different anticancer agents for fighting with solid tumors. Surprisingly, these scaffolds are able to deliver different combinations of drugs and agents, such as nanoparticles and release them in a time dependent manner. Here in this review, we summarize the principles of electrospinning and their uses in entrap-ment of drugs and anti-proliferative agents suitable for cancer therapy. The latest studies performed on treating cancer using electrospinning are mentioned and their advantages and disadvantages over con-ventional treatment methods are discussed. © 2020 Bentham Science Publishers

A facile one-step preparation of Ca10(PO4)6(OH)2/Li-BioMOFs resin nanocomposites with Glycyrrhiza glabra (licorice) root juice as green capping agent and mechanical properties study

The Ca10(PO4)6(OH)2/Li-BioMOFs resin nanocomposites were prepared and introduced as a new dental resin nanocomposite. Ca10(PO4)6(OH)2/Li-BioMOFs resin nanocomposites were synthesized with individual mechanical properties in the presence of lecithin as a biostabilizer. The hydrothermal synthesis of hydroxyapatite (HAp) nanostructures occurred in the presence of Glycyrrhiza glabra (liquorice) root juice that acts not only as a green capping agent but also as a reductant compound with a high steric hindrance agent. Results showed that the mechanical properties of nano-Ca10(PO4)6(OH)2 structures with a concentration of 60 ppm Li-BioMOF were increased by �132.5 MPa and 11.5 GPa for the flexural and Young�s modulus, respectively. Based on the optical absorption ultraviolet-visible spectrum, the HAp nanocrystallites had a direct bandgap energy of 4.2 eV. The structural, morphological, and mechanical properties of the as-prepared nanoparticles were characterized with the FT-IR (Fourier-transform infra-red), UV-Vis (ultraviolet visible) spectrums, X-ray diffraction, SEM (scanning electron microscopy), and TEM (transmission electron microscopy) images, and atomic force microscopy (AFM). It is suggested that HAp structures loaded on the Li-BioMOFs are as a suitable and novel substrate which can be considered as a promising biomaterial in dental resin nanocomposites significantly improved the strength and modulus. © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

Eco friendly synthesis of the LiY(MoO4)2 coral-like quantum dots in biotemplate MOF (QD/BioMOF) for in vivo imaging and ibuprofen removal from an aqueous media study

Here as the first new research study on the bio quantum dots on metal�organic frameworks (BQDMOFs), we synthesized coral-like LiY(MoO4)2 BQDMOFs with hydrothermal method as an eco-friendly and cost effective approach. The lecithin as complex mixtures of lipids such as phospholipids which was extracted from egg yolk was applied not only as emulsifier for the formation of LiY(MoO4)2 quantum dots, but also acts as organic ligand and auxiliary linker agent on the base structure of the organic frameworks. The effect of such factors as pH (3�11), show with increasing pH from 3 up to 11 solubility increases and as a result the ibuprofen removal increases.The BQDMOFs structures were synthesized on the base of an organic frameworks with coral-like morphology with diameter in a range about 8�20 nm. The physicochemical properties, Ibuprofen removal from an aqueous media and in vivo imaging studies were investigated on the LiY(MoO4)2 BQDMOFs structures. Final products were characterization with analyze nanoparticle size using the well-known technique of dynamic light scattering (DLS), High-performance thin-layer chromatography (HPTLC), Powder X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Infrared spectroscopy (IR) and the UV�Visible spectrum. From the sum of the results it can be established that LiY(MoO4)2 BQDMOFs structures acts not only as a super-radiant photoluminescence (SRP) substance for use in in vivo imaging in small animal researches but also as a surface-specific material which can be use in removal of ibuprofen drug from an aqueous media. The photocatalyst results of the LiY(MoO4)2 BQDMOFs shows high removal efficiency of ibuprofen (more than 99) after 60 min under the UV light. © 2020 The Author