Phylogenetic analysis of selected plant secondary product glycosyltransferases

The neighbor-joining tree was calculated with the Treecon software package (). Distance calculation was performed with Poisson correction and insertions/deletions were not taken into account. The tree was rooted using a sterol glycosyltransferase from (AsSGT) as an outgroup. Branch lengths indicate the number of substitutions per site. Bootstrap analysis was performed with 100 replicates and only values above 50% are shown. GenBank accession numbers and sources for the respective protein sequences are: AtUGT73B4, AAD17393 (); AtUGT73B5, AAD17392 (); AtUGT73B2, AAR01231 (); DicGT4, BAD52006 (); DbBet5GT, CAB56231 (); SbF7G, BAA83484 (); Letwi1, CAA59450 (); NtIS10a, AAB36652 (); NtIS5a, AAB36653 (); FaGT7, ABB 92749 (×); AtUGT71C2, AAC35238 (); AtUGT71D1, AAC35239 (); DicGT2, BAD52004 (); DbBet6GT, AAL57240 (); AtUGT71B6, BAB02840 (); FaGT6, ABB92748 (×); FaGT3, AAU09444 (×); NtGT3, BAB88934 (); NtGT1b, BAB60721 (); NtGT1a, BAB60720 (); NtSalGT, AAF61647 (); AtUGT84B1, AAB87119 (); AtUGT84B2, AAB87106 (); CuLimGT, BAA93039 (); FaGT2, AAU09443 (×); FaGT5, ABB92747 (); AtUGT84A3, CAB10327 (); AtUGT84A1, CAB10326 (); AtUGT84A2, BAB02351 (); BnSinGT, AAF98390 (); FaGT4, AAU09445 (×); PhA3RhaT, CAA81057 (); Ip3GGT, BAD95882 (); In3GGT, BAD95881 (); GtF3GT, BAA12737 (); DicGT3, BAD52005 (); VmUFGT1, BAA36972 (); PhF3GalT, AAD55985 (); DicGT1, BAD52003 (); VvGT1, AAB81682 (); FaGT1, AAU09442 (×); AtUGT78D1, NP_564357 (); AtUGT78D2, NP_197207 (); AsSGT, CAB06081 ().<p><b>Copyright information:</b></p><p>Taken from "Multi-substrate flavonol -glucosyltransferases from strawberry () achene and receptacle"</p><p></p><p>Journal of Experimental Botany 2008;59(10):2611-2625.</p><p>Published online 17 May 2008</p><p>PMCID:PMC2486459.</p><p></p

Sequence alignment of quercetin and kaempferol -glucosyltransferases, including two already crystallized proteins from (VvGT1) and (UGT71G1) as well as × GT6 (FaGT6), and × GT7 (FaGT7)

The alignment was performed using ClustalX (). Conserved amino acids are shaded, amino acids within 5 Å to kaempferol in the protein model of VvGT are boxed.<p><b>Copyright information:</b></p><p>Taken from "Multi-substrate flavonol -glucosyltransferases from strawberry () achene and receptacle"</p><p></p><p>Journal of Experimental Botany 2008;59(10):2611-2625.</p><p>Published online 17 May 2008</p><p>PMCID:PMC2486459.</p><p></p

Gene expression of (A) and (B) in achenes (black columns) and the receptacle (open columns) of small-sized green (G) and red (R) fruits

Transcript levels were analysed by qPCR as described in Materials and methods. Gene expression is shown as relative expression normalized with receptacle tissue from small-sized green fruit (G). Hormonal control of (C) and (D) gene expression. The achenes were carefully removed at mid-sized green stage and the fruits were harvested after 5 d. Additionally, deachened green fruits were treated with a lanolin paste containing the synthetic auxin NAA. Gene expression was analysed by qPCR as described in Materials and methods and the data were normalized against untreated strawberries with the achenes still attached to the fruit.<p><b>Copyright information:</b></p><p>Taken from "Multi-substrate flavonol -glucosyltransferases from strawberry () achene and receptacle"</p><p></p><p>Journal of Experimental Botany 2008;59(10):2611-2625.</p><p>Published online 17 May 2008</p><p>PMCID:PMC2486459.</p><p></p