[Hosta sp.]

原著和名: [記載なし]科名: ユリ科 = Liliaceae採集地: 宮崎県 東臼杵郡 大崩山 (日向 東臼杵郡 大崩山)採集日: 1973/8/1採集者: 萩庭丈壽整理番号: JH003752国立科学博物館整理番号: TNS-VS-953752備考: DB作成協力会による補足あ

Additional file 8: of Deep landscape update of dispersed and tandem repeats in the genome model of the red jungle fowl, Gallus gallus, using a series of de novo investigating tools

Diversity of CR1 within galGal4. The clustering of CR1 copies into subfamilies was re-investigated using SiLiX. Eight subfamilies were found, 7 of them matching with the Repbase sub-families CR1-C, CR1-D, CR1_F, CR1-G, CR1_GG, CR1-H, and CR1-Y. Their respective abundance in galGal4 was summarized in Table S2. (ODT 30 kb

Mitochondrial gene expression is altered in p43−/− testis at P3.

<p>The volcano plot is an arbitrary representation (proposed by the web-based RT² prolifer PCR Array Data Analysis program) of the fold change (FC) for each of the 84 genes in the array. It represents the log2C FC of each gene expression between the p43−/− group (n = 6) and the control group (n = 4) versus the negative Log10 P values from the t-test. The red vertical line indicates that the gene expression fold change threshold is 2. The blue horizontal line indicates that the P value of the T-test threshold is 0.05. Genes, which were significantly up-regulated in p43−/− mice in comparison with controls, were indicated.</p

Percentage of <i>in vivo</i> proliferating Sertoli cells in p43−/− testis at P3 and P10.

<p>A) Immunohistochemical labelling of proliferating cells revealed by BrdU incorporation injected three hours before sacrifice in P3 and P10 testis of p43−/− mice. Arrowheads: Sertoli cells; arrows: germ cells; star: myoid peritubular cells; black: BrdU-positive; white: BrdU-negative. Scale bar 10 µm. B) BrdU negative and positive Sertoli cells were counted and proliferation index was calculated. In p43−/− testis, SC proliferation was increased at P3 in comparison to WT mice (P<0.001). At P10 there is no difference. (n = 3/genotype/age). Data are shown as the mean+/− SEM; statistical analyses; two-way ANOVA followed by Bonferroni’s post-test.</p

The mitochondrial morphology is unaffected in germ cells of p43−/− mice.

<p>Mitochondria observed in germ cells in the adult testis of wild type mice (WT) and in the adult testis of p43−/− mice at 5 months of age (p43−/−). Mitochondria present in germ cells of p43−/− mice were morphological identical at those presenting in germ cells of WT mice. Data are shown as the mean+/− SEM; statistical analyses; two-way ANOVA followed by Bonferroni’s post-test (n = 60). Arrowheads: Mitochondria.</p

CDK4 expression is increased in P43−/− testis at P3.

<p>Western blot analyses were performed with p43−/− and WT proteins extract testes at P3 (n = 5 for P43−/−, n = 4 for WT) using a specific CDK4 and ß-actin antibodies. Normalization was achieved using ß-actin levels. Data are shown as the mean+/− SEM and statistical analyses were performed using the student t test. CDK4 level was increased (P<0,001).</p

Cell cycle gene expression is impacted in p43−/− testis at P3.

<p>The volcano plot is an arbitrary representation (proposed by the web-based RT² prolifer PCR Array Data Analysis program) of the fold change (FC) for each of the 84 genes in the array. It represents the log2 FC of each gene expression between the p43−/− group (n = 6) and the control group (n = 8) versus the negative Log10 P-values from the t-test. The red vertical line indicates that the gene expression fold change threshold is 2. The blue horizontal line indicates that the P-value of the T-test threshold is 0.05. Genes, which were significantly upregulated in p43−/− in comparison with controls, were indicated.</p

Mitochondrial gene expressions measured by Q-PCR.

<p>For each gene: fold values (FC) and p value obtained in p43−/− mice versus respective controls. Significant genes changed in p43−/− mice are in bold. ND: gene expression was no detected.</p

Cell cycle gene expressions measured by Q-PCR.

<p>For each gene: fold values (FC) and p value obtained in p43−/− mice versus respective controls. Significant genes changed in p43−/− mice are in bold. ND: gene expression was no detected.</p

Müller glial cells from transgenic mice are more resistant to iron-mediated stress than Müller glial cells from wild-type mice

A: Culture of Müller glial (MG) cells from wild-type (WT) and transgenic (Tg) mice at the first passage were treated with 100 µM of FeCl-NTA (FN), during 96 h. The number of cells was evaluated by counting in comparison to control condition. Each column represents the mean ±SEM. The triple asterisk represent statistical significance of differences between treated and control, respectively, for WT and Tg, and between control and treated, p<p><b>Copyright information:</b></p><p>Taken from "The protective role of transferrin in Müller glial cells after iron-induced toxicity"</p><p></p><p>Molecular Vision 2008;14():928-941.</p><p>Published online 20 May 2008</p><p>PMCID:PMC2391081.</p><p></p