Sample characteristics by <i>FTO</i> rs9939609 genotype (n = 1718).

a<p>mean (standard deviation) unless otherwise indicated.</p>b<p>median (100% range).</p>c<p>with the chi-square statistic for categorical variables, one-way ANOVA for normally distributed continuous variables and the Kruskal-Wallis test for non-normally distributed.</p><p>continuous variables.</p>d<p>overweight or obesity is defined as a BMI-sds >1.10.</p

Associations of child <i>FTO</i> at rs9939609 with child eating behaviour at 4 years (n = 1718).

<p>Abbreviations: OR, odds ratio; 95%CI, 95% confidence interval; ref, reference.</p>a<p>The ORs represent the increased risk of high scores on CEBQ subscales per <i>FTO</i> minor allele at rs9939609.</p

Associations of child <i>FTO</i> at rs9939609 with child executive function at 4 years.

<p>Abbreviations: OR, odds ratio; 95%CI, 95% confidence interval; ref, reference.</p>a<p>The ORs represent the increased risk of clinical scores on BRIEF-P Emotional Control and Inhibition per <i>FTO</i> minor allele at rs9939609.</p

Correlation between child BMI, child emotional and behavioural problems, executive function and eating behaviour.

<p>Spearman correlation coefficient (two tailed).</p>*<p><0.05, **<0.01 ***<0.001.</p

Association with morning plasma cortisol of SNPs representing signals in the <i>SERPINA6/SERPINA1</i> region from meta-analyses of discovery genome-wide association studies and of replication studies.

a<p>adjusted for age and sex.</p>b<p>rs2749527 was replaced with rs2749529 (r² = 0.905, D' = 1.0) as rs2749527 failed manufacture for replication. LD patterns (from SNAP HapMap CEU build 22): rs11621961-rs12589136 (r² = 0.131), rs11621961-rs2749529 (r² = 0.260), rs12589136-rs2749529 (r² = 0.291), rs11621961-rs2749527 (r² = 0.255). Independent SNPs were defined by PLINK using the clumping function (within 500kb, LD r² >0.2, P-value <5x10⁻⁵)</p

Meta-analysis of genome wide association studies for morning plasma cortisol.

<p>A) Manhattan plot of −lop₁₀<i>P</i> values by chromosome. The red horizontal line indicates genome-wide significance (<i>P</i><5×10⁻⁸) and the blue horizontal line indicates moderate significance (<i>P</i><5×10⁻⁵). The lead SNP rs12589136 (chr14:94,793,686; b37) in red is genome-wide significant. SNPs within ±50 kb of cortisol-related candidate genes (listed in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004474#pgen.1004474.s006" target="_blank">Table S6</a>) are highlighted in colours. B) Quantile-quantile plot of −log₁₀<i>P</i>, comparing the distribution of observed −log₁₀<i>P</i>-values and that expected by chance.</p

Forest plot of association of morning plasma cortisol with rs12589136.

<p>Plot shows association as beta values with 95%CI for morning plasma cortisol z-scores for rs12589136 (T allele) in discovery cohorts (blue) and meta-analysis (red).</p

Functional consequences of variants in the <i>SERPINA6/A1</i> locus significantly associated with morning plasma cortisol in GWAMA, and of the Leuven variant, in CROATIA-Korcula.

a<p>Data for total cortisol is from the whole Croatia-Korcula sample, n = 898.</p>b<p>Samples selected for measured free cortisol assay were age and sex matched homozygotes at rs12589136.</p>c<p>adjusted for age, sex, and first three principal components, using kinship matrix derived from GWAS data.</p><p>Data are geometric mean (95% CI)[n]. Cortisol values are nmol/L, CBG µmol/L, cortisol binding activity nmol/L, and α1-antitrypsin g/L. RCL = reactive centre loop.</p

Regional associations surrounding lead SNP rs12589136 in genome-wide meta-analysis of morning plasma cortisol.

<p>Regional plot shows −log₁₀<i>P</i> values of all SNPs, and degree of correlation between all SNPs and lead SNP rs12589136. SNPs with lower <i>P</i> values span <i>SERPINA6</i> and <i>SERPINA1</i> genes within a recombination boundary.</p