266 - Effects of Meiotic Drive on Developing Eyestalks in Stalk-Eyed Flies

Teleopsis dalmanni, also known as the Malaysian stalk-eyed fly, exhibits sexually dimorphic eyestalks, which means there is a difference between male and female eyestalk length. Additionally, there are differences in eyestalk length within male populations. One possible reason for these differences is that some stalk-eyed flies exhibit meiotic drive. Meiotic drive is a selfish allele on the X chromosome that violates Mendel’s Law of Segregation by increasing its own transmission. Males with meiotic drive have shorter eyestalks, produce more female offspring, and have lower fitness because females preferentially mate with males that have longer eyestalks. We are working to determine what genes are differentially expressed due to the presence of meiotic drive in developing eye discs that are causing differences between standard and drive male eyestalk length. We are also attempting to determine if meiotic drive is affecting eyestalk development in the same way for males and females, and whether the same genes are impacted. PCR and gel electrophoresis are being performed using primers that indicate sex and drive status. These samples are then grouped into pools and sent for RNA sequencing. Female RNA samples are being analyzed using the differential gene expression software, Kallisto and DESeq2. Previous research indicates an upregulation of gene expression in standard males and a downregulation in gene expression in males with drive. Based on the data from male flies, it’s predicted that there will also be a downregulation in gene expression in eye discs in females with drive compared to standard females

Being a Child Today Is Simply Not Easy

Issue Editor, Ira Colby and Catherine M. Flaitz\u27s, point of view and summary of the articles in Accountable Communities: Healthier Neighborhoods, Healthier Children

Making a Community Ready for Oral Health

The challenges of resolving the oral health disparities among children and other vulnerable populations requires a well-organized community safety net, along with a sincere commitment to prioritize the elimination of this common, but preventable, dental disease. Adequate funding for basic health needs, while incorporating oral health care into a comprehensive primary care model in our neighborhoods provides the best chance for a successful outcome. In order to build better communities for our children, improvement in the access and delivery of dental care must be part of the equation

Expression of Keratinocyte Growth Factor in Periapical Lesions

The epithelial proliferation associated with inflammatory periapical lesions and with periapical cyst formation represents an interesting but poorly understood pathological change. Keratinocyte growth factor (KGF) is a recently identified growth factor that is produced by stromal fibroblasts and acts specifically to stimulate epithelial growth and differentiation. To investigate its possible role in the activation of the normally quiescent rests of Malassez, we examined the expression of KGF by in situ hybridization of sections of normal periodontal ligament (PDL) and of 12 periapical granulomas or cysts. Normal PDL and periapical granulomas with scant inflammatory infiltration showed few cells expressing message for KGF. However, KGFexpressing cells were found in the connective tissue stroma close to dense foci of inflammatory cells and to proliferating epithelial elements and cystic epithelial linings. Examination of tissues by the reverse-transcription polymerase chain reaction (RT-PCR) showed KGF expression in 4 specimens of periapical lesions but low or undetectable levels in normal PDL. These observations suggest that the induction of KGF expression in the stromal cells of periapical lesions may play an important role in stimulating the epithelial proliferation associated with cyst formation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66683/2/10.1177_00220345960750090701.pd

Epithelioid hemangioendothelioma of the mandibular gingiva: A rare case of metastasis 4 years after radical excision and literature review

Malignant epithelioid hemangioendothelioma (MEH), or high-risk epithelioid hemangioendothelioma, is a low- to intermediate-grade vascular malignancy. A few cases of MEH have been documented in the head and neck region, including the neck, thyroid gland, larynx and scalp. MEHs are extremely rare in the oral cavity. Only 31 cases of MEH in the oral cavity were described in English literature between 1975 and 2014. Further, only eleven cases were referred to MEH of the maxillary or mandibular gingiva. No gingival MEH metastases have been described in literature. We report a literature review and a case of MEH with a metastatic occurrence 4 years after surgical excision

Lingual juvenile xanthogranuloma in a woman: a case report

<p>Abstract</p> <p>Introduction</p> <p>Juvenile xanthogranuloma is a rare non-Langerhans cell histiocytosis that usually occurs during infancy and early childhood. The presence of single or multiple raised cutaneous lesions characterize this self-healing disorder. Extracutaneous sites are rare.</p> <p>Case presentation</p> <p>We present a rare case of oral juvenile xanthogranuloma in a 49-year-old Caucasian woman. The histopathologic diagnosis of the lingual neoformation was histiocitary proliferation with the presence of giant cells, Touton type, compatible with juvenile xanthogranuloma.</p> <p>Conclusion</p> <p>To establish an accurate diagnosis, microscopic evaluation and immunohistochemical staining are necessary. Dentists, dermatologists and general practitioners may be the first to recognize this rare condition during the inspection of the oral cavity.</p

Buccal soft tissue lipoma in an adult Nigerian: a case report and literature review

<p>Abstract</p> <p>Introduction</p> <p>Lipomas are benign mesenchymal neoplasms composed of mature adipocytes, usually surrounded by a thin fibrous capsule. They are uncommon intra-oral tumors with 1% to 4% occurring in this region. The literature is scanty on lipomas occurring in the buccal soft tissue, especially in our environment.</p> <p>Case presentation</p> <p>We present a case of a 35-year-old woman of the Tiv ethnic group of Nigeria who presented with a slow growing left cheek swelling that was treated by intra-oral local excision.</p> <p>Conclusion</p> <p>The purpose of this report is to highlight the existence of this rare but not uncommon disease even in our environment and to emphasize that a high index of suspicion is needed in making a diagnosis. Surgical excision as treatment is associated with an excellent outcome.</p

Plasmablastic lymphoma mimicking orbital cellulitis

Introduction Orbital cellulitis is an uncommon, potentially devastating condition that, when not promptly and adequately treated, can lead to serious sequelae. The presenting clinical signs are proptosis, swelling, ophthalmoplegia, pain and redness of the peri-orbital tissues. A number of case