Islet viability <i>in vitro</i>.

<p>A decrease in islet damage was observed in different assays after islet isolation supplemented with 10 mM GEE. (A) Membrane integrity evaluated with Syto Green/Ethidium Bromide (P<0.05). (B) Islet apoptosis was measured with TUNEL staining after islet isolation. (P<0.05). (C, D, E and F) Fractional beta-cell viability staining using Newport Green, 7-aminoactinomycin D (7-AAD) and tetramethylrhodamine ethyl ester (TMRE) (P<0.05).</p

Intracellular reactive oxygen species after islet isolation.

<p>There is a greater percentage of increased fluorescence intensity (High ROS) measured by the excitation and emission of carboxy-h2DCFDA at 495/529 nm (FITC channel), in the control mouse islets (B) when compared to the GEE treated islets (D). ROS content in the control cells (56.9+/−4.33) is significantly higher than in the treated cells (46.98+/−3.94) (p<0.005) (C). Panel A is depicting unstained islets. N = 7 islet isolations.</p

Human islets after 24 h culture on different GEE concentrations.

<p>Intracelullar ROS was evaluated using carboxy-H₂DCFDA and flow cytometry. Membrane integrity was evaluated by Syto green/Ethidium bromide. Fractional beta-cell viability was assessed using Newport Green, 7-AAD and TMRE.</p>*<p><i>p</i><0.05;</p>**<p><i>p</i><0.01;</p>***<p><i>p</i><0.001 in comparison to control.</p

Percentage of normoglycemic mice after marginal mass transplant (150 islets transplanted under the renal cavity in diabetic, streptozotocin treated mice) with islets treated (GEE) or not (Control) with glutathione-ethyl-ester (P<0.05).

<p>Percentage of normoglycemic mice after marginal mass transplant (150 islets transplanted under the renal cavity in diabetic, streptozotocin treated mice) with islets treated (GEE) or not (Control) with glutathione-ethyl-ester (P<0.05).</p