Mosaic Analysis of Precursors of Nerve, Bone and Vasculature in Caudal Body and Fin of Zebrafish

This project focuses on the specific developmental origin and formation of pre-osteoblasts, vascular cells and sensory neurons in the caudal region and tail of zebrafish. Our objective is to generate genetically marked clones using transposon microinjections and to analyze fluorescent protein markers in deep body and tail cell populations. If clones are shared between the fin and deep body it is suggestive that these cells share a common progenitor cell. Zebrafish fins can serve as a model for human organogenesis because conserved genes and cell types are found in human limbs

Biomass and Productivity of Thalassia testudinum in Estuaries of the Florida Panhandle

Thalassia testudinum often dominates seagrass meadows of the Florida panhandle but few measurements of productivity, biomass, density, turnover or leaf area index in this region have been made. We targeted 5 estuaries located at similar latitudes, 30⁰ ± 0.3⁰N: Big Lagoon, Santa Rosa Sound, St. Andrew Bay, St. Joseph Bay, and St. George Sound. This study was one component of a collaborative partnership of state and local researchers examining factors preventing recovery in panhandle estuarine areas that had historically contained seagrass in the 1940s and 1950s. Measurements were made twice in 2016, once in June and then again in summer or fall, except in Santa Rosa Sound where measurements were made 3 times. In the estuaries sampled for the second time in July or August, aboveground productivity was greater than in June. St. Joseph Bay had the highest aboveground productivity (4.3 g/m2/d) and 1—sided leaf area index (4.2) while St. George Sound had the lowest values (0.41 g/m2/d and 1.0). Principal component analysis suggested that St. Andrew Bay, Big Lagoon and Santa Rosa Sound were the most similar, with higher values for shoot densities and leaf turnover and lower salinities and watershed:water ratios. St. Joseph Bay had high aboveground productivity and salinity, and low turbidity. St. George Sound had low aboveground productivity, high total suspended solids and the highest watershed:water ratio. These baseline productivity estimates will be useful to assess the success of restoration efforts targeting seagrasses in the Florida panhandle and evaluate impacts of climate change on seagrasses

Using changes in biomass and productivity to discern anthropogenic impacts in aquatic ecosystems

The purpose of these studies was to monitor changes in two aquatic ecosystems that represent end members along a continuum of human impacts. St. Andrew Bay in Panama City, Florida, USA, has been impacted by humans since it formed about 5,000 years ago; however these impacts have accelerated in the last 150 years as industrialization took place. In contrast, the peatlands north of High Level, Alberta, Canada, are located in a region where human population and development are minimal, yet these remote areas do not appear to be immune to the global climate change that resulted from the industrial revolution. This work describes the effects of water quality on seagrass distribution and epiphyte growth in St. Andrew Bay and it shows how climate change affects peat deposits north of High Level. Water quality has been monitored in St. Andrew Bay since 1990 and these data were coupled with seagrass monitoring data collected since 2000 and five aerial photos taken since 1953 to better determine the extent of seagrass losses in the bay system. The St. Andrew Bay system is composed of four smaller bays: West Bay, North Bay, St. Andrew Bay, and East Bay, and although there has been no systemic decline in seagrass coverage in North Bay, St. Andrew Bay, and East Bay, approximately half of the seagrasses in West Bay have been destroyed or degraded since 1953. Comparisons among these smaller bays show higher turbidities, higher chlorophyll a concentrations, and increased epiphyte growth rates in West Bay which result in shallower seagrass depths. Although the initial cause of seagrass loss in West Bay is unknown, the present eutrophication of this area will make it harder for seagrasses to recover. Furthermore, the future development of over 30,000 acres within West Bay's watershed surrounding a new international airport and industrial complex does not bode well for this stressed ecosystem. Although the peatlands of Canada are located in an area where human impacts are minimal, these ecosystems are still at risk from indirect stressors such a global climate change. Peatlands formed approximately 7,000 years ago as shallow lakes filled in with vegetation; eventually the accumulating vegetation insulated the ground allowing permafrost to form. Over the past 60 years however, global temperatures have increased, the direct result of increased carbon dioxide levels that started to climb after the industrial revolution. This warmer climate decreases the ability of peat to sufficiently insulate the ground allowing the permafrost to melt. Relatively small, shallow collapse scar bogs have now formed within the permafrost plateau and this creates wet depressions where primary productivity increases. Peat cores were removed from several bogs north of High Level, Alberta, and the age of the successive layers in the peat were determined using 210Pb and the Constant Rate of Supply (CRS) model. Ages derived from the activity of 137Cs in two cores were used to corroborate these results. Peat accumulation rates were determined for each layer in the core based on peat age and cumulative mass depth of the layer. In general, peat accumulation was greatest in bogs 60 miles north of High Level and lowest in bogs 120 miles away. Furthermore, when peat accumulation rates were compared among neighboring cores, changes in peat accumulation rates occurred at similar time intervals. This indicates that local climate factors influence the rate of peat accumulation once these collapse scar bogs form; however, global changes in climate appear to be responsible for the initial formation of these bogs