The synapsin gene family in basal chordates: evolutionary perspectives in metazoans

<p>Abstract</p> <p>Background</p> <p>Synapsins are neuronal phosphoproteins involved in several functions correlated with both neurotransmitter release and synaptogenesis. The comprehension of the basal role of the synapsin family is hampered in vertebrates by the existence of multiple synapsin genes. Therefore, studying homologous genes in basal chordates, devoid of genome duplication, could help to achieve a better understanding of the complex functions of these proteins.</p> <p>Results</p> <p>In this study we report the cloning and characterization of the <it>Ciona intestinalis </it>and amphioxus <it>Branchiostoma floridae </it>synapsin transcripts and the definition of their gene structure using available <it>C. intestinalis </it>and <it>B. floridae </it>genomic sequences. We demonstrate the occurrence, in both model organisms, of a single member of the synapsin gene family. Full-length synapsin genes were identified in the recently sequenced genomes of phylogenetically diverse metazoans. Comparative genome analysis reveals extensive conservation of the SYN locus in several metazoans. Moreover, developmental expression studies underline that synapsin is a neuronal-specific marker in basal chordates and is expressed in several cell types of PNS and in many, if not all, CNS neurons.</p> <p>Conclusion</p> <p>Our study demonstrates that synapsin genes are metazoan genes present in a single copy per genome, except for vertebrates. Moreover, we hypothesize that, during the evolution of synapsin proteins, new domains are added at different stages probably to cope up with the increased complexity in the nervous system organization. Finally, we demonstrate that protochordate synapsin is restricted to the post-mitotic phase of CNS development and thereby is a good marker of postmitotic neurons.</p

Phenylalanine Butyramide Is a New Cosmetic Ingredient with Soothing and Anti-Reddening Potential

Human skin is colonized by diverse commensal microbes, making up the skin microbiota (SM), contributing to skin integrity and homeostasis. Many of the beneficial effects aroused by the SM are exerted by microbial metabolites such as short-chain fatty acids (SCFAs), including butyric acid. The SCFAs can be used in cosmetic formulations against skin diseases to protect SM by preserving and/or restoring their natural balance. Unpleasant sensorial properties and unfavorable physicochemical properties of butyrate strongly limit its cosmetic use. In contrast, some butyrate derivatives, including phenylalanine butyramide (C13H18N2O2 , FBA), a solid form of butyric acid, are odorless while retaining the pharmacokinetic properties and safety profile of butyric acid. This study assessed the FBA’s permeation across the skin and its soothing and anti-reddening potential to estimate its cosmetic application. The dosage method used to estimate FBA’s levels was validated to be sure of analytical results. The FBA diffusion tests were estimated in vitro using a Franz-type vertical diffusion cell. The soothing action was evaluated in vivo by Colorimeter CL400, measuring the erythema index. The results suggest that the FBA represents an innovative way to exploit the benefits of butyric acid in the cosmetic fields since it cannot reach the bloodstream, is odorless, and has a significative soothing action (decrease the erythema index −15.7% after 300 , and −17.8% after 600 )

Differential responses of zooplankton assemblages to environmental variation in temporary and permanent ponds

Permanent and temporary wetlands in Mediterranean shrublands represent unique repositories of biodiversity, which are increasingly threatened by human-induced habitat loss. The zooplankton of a permanent (P1) and a temporary pond (T35) in the Natural Reserve of Castelporziano, a rare residual stretch of such a shrubland in Central Italy (Latium), was investigated to: (1) expand and deepen knowledge of these endangered freshwater habitats, which represent a crucial component of Mediterranean biodiversity; (2) identify environmental controls regulating the development of zooplankton communities of each environment; and (3) highlight differences in the adaptive responses of the zooplankton community in relation to the different ecological conditions experienced by permanent and temporary habitats. Despite summer desiccation in T35, the two ponds exhibited a relative homogeneity in hydrological and physico-chemical dynamics. Zooplankton assemblages contained 41 total taxa, of which 32 were found in P1 and 28 in T35. Out of the 41 taxa identified, 22 (> 50%) were exclusively present in one of the two ponds. On a yearly basis, the community dynamics of P1 seemed to be conditioned by physical and chemical factors and by hydrological cycle characteristics, while the community of T35 responded to algal blooms, food competition and predator/prey equilibria rather than correlating to abiotic factors. The main differences amongst zooplankton assemblages were observed over short time scales and occurred both within and between seasons, highlighting the role of some structural taxa that dominated the average composition of the community throughout the year, and the importance of "quick-response" taxa in determining the short-term composition and structure variation of pond zooplankton. A year-round cyclic community succession peculiar to each pond is described

Habitat features and distribution of <i>Salamandra salamandra</i> in underground springs

Subterranean habitats are among the less known terrestrial habitats, but can reveal an unexpected biodiversity, and can play an underestimated role for amphibians. The fire salamander <i>Salamandra salamandra</i> is sometimes found in underground environments, but the factors affecting its distribution in subterranean spaces remain substantially unexplored. We repeatedly surveyed some hypogeous springs, such as draining galleries and “bottini” in NW Italy, in order to evaluate the relationship between environmental features and distribution of <i>S. salamandra</i> in these underground springs. We performed visual encounter surveys to assess the occurrence of larvae, juveniles or adults in springs. We also recorded four habitat variables: easy of access, isolation, macrobenthos richness and forest cover of the surrounding landscape. We used generalized linear models to evaluate the relationships between habitat features and occurrence of larvae. We observed larvae of <i>S. salamandra</i> in 13 out of 22 springs; their presence was associated to springs with high easy of access and with relatively rich macrobenthos communities. In underground springs, larval development apparently required longer time than in nearby epigeous streams. Nevertheless, S. salamandra can attain metamorphosis in this environment. The occurrence of <i>S. salamandra</i> in underground environments was not accidental, but repeated in the time and interesting from an ecological point of view, confirming the high plasticity of the species