Molecular lesions associated with white gene mutations induced by I-R hybrid dysgenesis in Drosophila melanogaster

We have identified molecular lesions associated with six mutations, w(IR2) and w(IR4-8), of the white gene of Drosophila melanogaster. These mutations arose in flies subject to I-R hybrid dysgenesis. Four of the mutations give rise to coloured eyes and are associated with insertions of 5.4-kb elements indistinguishable from the I factor controlling I-R dysgenesis. The insertion associated with w(IR4) is at a site which, within the resolution of these experiments, is identical to that of two previously studied I factors. This appears to be a hot-spot for I factor insertion. We have compared the sites of these insertions with sequences complementary to white gene mRNA identified by Pirrotta and Bröckl. The hot-spot is in the fourth intron. The insertion carried by w(IR5) is either within, or just beyond, the last exon. The insertion carried by w(IR6) is near the junction of the first exon and first intron. The w(IR2) mutation is a derivative of w(1). It contains an insertion of I factor DNA within, or immediately adjacent to, the F-like element associated with w(1), and results in restoration of some eye colour. This insertion is just upstream of the start of the white mRNA. Mutations w(IR7) and w(IR8) are deletions removing mRNA coding sequences. Both determine a bleached white phenotype

STS-26 solid rocket booster post flight structural assessment

A post flight assessment of the Space Shuttle's Solid Rocket Boosters was conducted at the John F. Kennedy Space Center in Florida after the launch of STS-26. The two boosters were inspected for structural damage and the results of this inspection are presented. Overall, the boosters were in good condition. However, there was some minor damage attributed to splash down. Some of this damage is a recurring problem. Explanations of these problems are provided

Spacecraft cost estimation

Spacecraft subsystem cost estimatio

Different Motion Cues Are Used to Estimate Time-to-arrival for Frontoparallel and Loming Trajectories

Estimation of time-to-arrival for moving objects is critical to obstacle interception and avoidance, as well as to timing actions such as reaching and grasping moving objects. The source of motion information that conveys arrival time varies with the trajectory of the object raising the question of whether multiple context-dependent mechanisms are involved in this computation. To address this question we conducted a series of psychophysical studies to measure observers’ performance on time-to-arrival estimation when object trajectory was specified by angular motion (“gap closure” trajectories in the frontoparallel plane), looming (colliding trajectories, TTC) or both (passage courses, TTP). We measured performance of time-to-arrival judgments in the presence of irrelevant motion, in which a perpendicular motion vector was added to the object trajectory. Data were compared to models of expected performance based on the use of different components of optical information. Our results demonstrate that for gap closure, performance depended only on the angular motion, whereas for TTC and TTP, both angular and looming motion affected performance. This dissociation of inputs suggests that gap closures are mediated by a separate mechanism than that used for the detection of time-to-collision and time-to-passage. We show that existing models of TTC and TTP estimation make systematic errors in predicting subject performance, and suggest that a model which weights motion cues by their relative time-to-arrival provides a better account of performance

Differential parent and teacher reports of school readiness in a disadvantaged community

Differential ratings by multiple informants are an important issue in survey design. Although much research has focused on differential reports of child behaviour, discrepancies between parent and teacher reports of children’s school readiness are less explored.

One-to-one technology in middle school science

This paper reports on the ongoing digital implementation of one-to-one digital devices in the middle school science classroom, examining existing goals, the history and use, and offers a professional development course designed to engage teachers to improve their practices. This work examines teacher efficacy in the classroom and highlights teachers’ views on the emerging push for one-to-one student centered learning. Results suggest the importance of methodologies that increase student achievement and reduce teacher driven curriculum. The combination of teacher engagement through professional development and practice promote both teacher and students’ interest and learning goals with the use of one-to-one devices, the created professional development incorporates these ideas

Evaluating the Potential of Using 5-Azacytidine as an Epimutagen

A number of early flowering lines were induced when 5-azacytidine was applied to germinating flax (Linum usitatissimum L.) seed. The genetics of these lines indicate that the induced changes are epigenetic and probably result from demethylation of the genomic DNA at loci that affect flowering age. Although the growth and development of three stable early flowering lines are altered and the percentage of filled seed was reduced in all three lines compared with controls, measures of seed productivity demonstrated that harvest index was unaffected in two of the lines. In the third, harvest index was lower than normal and both seed set per capsule and seed mass per 100 seed were reduced. Furthermore, six generations after induction this line began to display relatively high levels of polyembryony. The late appearance of this twinning and other aspects related to working with lines induced by 5-azacytidine and using 5-azacytidine as an epimutagen are discussed

Climate change and the selective signature of the Late Ordovician mass extinction

Selectivity patterns provide insights into the causes of ancient extinction events. The Late Ordovician mass extinction was related to Gondwanan glaciation; however, it is still unclear whether elevated extinction rates were attributable to record failure, habitat loss, or climatic cooling. We examined Middle Ordovician-Early Silurian North American fossil occurrences within a spatiotemporally explicit stratigraphic framework that allowed us to quantify rock record effects on a per-taxon basis and assay the interplay of macrostratigraphic and macroecological variables in determining extinction risk. Genera that had large proportions of their observed geographic ranges affected by stratigraphic truncation or environmental shifts at the end of the Katian stage were particularly hard hit. The duration of the subsequent sampling gaps had little effect on extinction risk, suggesting that this extinction pulse cannot be entirely attributed to rock record failure; rather, it was caused, in part, by habitat loss. Extinction risk at this time was also strongly influenced by the maximum paleolatitude at which a genus had previously been sampled, a macroecological trait linked to thermal tolerance. A model trained on the relationship between 16 explanatory variables and extinction patterns during the early Katian interval substantially underestimates the extinction of exclusively tropical taxa during the late Katian interval. These results indicate that glacioeustatic sea-level fall and tropical ocean cooling played important roles in the first pulse of the Late Ordovician mass extinction in Laurentia