CoA protects against the deleterious effects of caloric overload in Drosophila

We developed a Drosophila model of T2D in which high sugar (HS) feeding leads to insulin resistance. In this model, adipose TG storage is protective against fatty acid toxicity and diabetes. Initial biochemical and gene expression studies suggested that deficiency in CoA might underlie reduced TG synthesis in animals during chronic HS feeding. Focusing on the Drosophila fat body (FB), which is specialized for TG storage and lipolysis, we undertook a series of experiments to test the hypothesis that CoA could protect against the deleterious effects of caloric overload. Quantitative metabolomics revealed a reduction in substrate availability for CoA synthesis in the face of an HS diet. Further reducing CoA synthetic capacity by expressing FB-specific RNAi targeting pantothenate kinase (PK orfumble) or phosphopantothenoylcysteine synthase (PPCS) exacerbated HS-diet-induced accumulation of FFAs. Dietary supplementation with pantothenic acid (vitamin B5, a precursor of CoA) was able to ameliorate HS-diet-induced FFA accumulation and hyperglycemia while increasing TG synthesis. Taken together, our data support a model where free CoA is required to support fatty acid esterification and to protect against the toxicity of HS diets

Development of digital computer program for thermal network correction. Phase 2 - Program development. Phase 3 - Demonstration/application Final report

Developing digital computer program for correcting soft parameters of thermal network by Kalman filtering metho

In vivo laser Doppler holography of the human retina

The eye offers a unique opportunity for non-invasive exploration of cardiovascular diseases. Optical angiography in the retina requires sensitive measurements, which hinders conventional full-field laser Doppler imaging schemes. To overcome this limitation, we used digital holography to perform laser Doppler perfusion imaging of the human retina in vivo with near-infrared light. Wideband measurements of the beat frequency spectrum of optical interferograms recorded with a 39 kHz CMOS camera are analyzed by short-time Fourier transformation. Power Doppler images and movies drawn from the zeroth moment of the power spectrum density reveal blood flows in retinal and choroidal vessels over 512 $\times$ 512 pixels covering 2.4 $\times$ 2.4 mm$^2$ on the retina with a 13 ms temporal resolution.Comment: 5 pages, 5 figure

An intelligent tutoring system for the investigation of high performance skill acquisition

The issue of training high performance skills is of increasing concern. These skills include tasks such as driving a car, playing the piano, and flying an aircraft. Traditionally, the training of high performance skills has been accomplished through the use of expensive, high-fidelity, 3-D simulators, and/or on-the-job training using the actual equipment. Such an approach to training is quite expensive. The design, implementation, and deployment of an intelligent tutoring system developed for the purpose of studying the effectiveness of skill acquisition using lower-cost, lower-physical-fidelity, 2-D simulation. Preliminary experimental results are quite encouraging, indicating that intelligent tutoring systems are a cost-effective means of training high performance skills

Cavity QED with separate photon storage and qubit readout modes

We present the realization of a cavity quantum electrodynamics setup in which photons of strongly different lifetimes are engineered in different harmonic modes of the same cavity. We achieve this in a superconducting transmission line resonator with superconducting qubits coupled to the different modes. One cavity mode is strongly coupled to a detection line for qubit state readout, while a second long lifetime mode is used for photon storage and coherent quantum operations. We demonstrate sideband based measurement of photon coherence, generation of n photon Fock states and the scaling of the sideband Rabi frequency with the square root of n using a scheme that may be extended to realize sideband based two-qubit logic gates.Comment: 4 pages, 5 figures, version with high resolution figures available at http://qudev.ethz.ch/content/science/PubsPapers.htm

Time reversal in thermoacoustic tomography - an error estimate

The time reversal method in thermoacoustic tomography is used for approximating the initial pressure inside a biological object using measurements of the pressure wave made on a surface surrounding the object. This article presents error estimates for the time reversal method in the cases of variable, non-trapping sound speeds.Comment: 16 pages, 6 figures, expanded "Remarks and Conclusions" section, added one figure, added reference