Additional file 3: Table S1. of Intrinsic factors and the embryonic environment influence the formation of extragonadal teratomas during gestation

Primer sequences. (DOCX 14 kb

Additional file 1: Figure S1. of Intrinsic factors and the embryonic environment influence the formation of extragonadal teratomas during gestation

ES cells differentiated in the absence of LIF give rise to PS-like cells in vitro. (A) Time-course qPCR expression analysis of ES- and PS/organiser-specific markers in wild type E14 ES cells cultured in the presence of serum and absence of LIF for the indicated amount of time. (B) Immunocytochemistry of PS/organiser marker expression in wild type E14 ES cells cultured in the presence of serum and absence of LIF for 4 days. (JPEG 943 kb

Additional file 2: Figure S2. of Intrinsic factors and the embryonic environment influence the formation of extragonadal teratomas during gestation

The induction of PS-like cells in vitro correlates with Venus upregulation in Tps/tb-Oct-Nanog-Venus ES cells. (A) Flow cytometry analysis of Venus expression in Tps/tb-Oct-Nanog-Venus ES cells after 4 days of differentiation in the presence of serum/Dox and absence of LIF. (B) Fluorescence microscopy of Venus expression Dox-treated and untreated Tps/tb-Oct-Nanog-Venus ES cells after 4 days of differentiation in the presence of serum/Dox and absence of LIF. (JPEG 607 kb