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    Abstracts of presentations on plant protection issues at the xth international congress of virology: August 11-16, 1996 Binyanei haOoma, Jerusalem Iarael part 3(final part)

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    2006. Production of polyclonal antibodies to a recombinant potato mop-top virus non-structural triple gene block protein 1

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    Abstract The coat protein (CP) coding regions of two Czech Potato mop-top virus (PMTV) isolates were sequenced and shown to be identical. One, the Korneta isolate CP gene, was cloned in several expression vectors. The recombinant PMTV-CP was expressed in Escherichia coli and the purified recombinant protein was used to produce PMTV-specific polyclonal antibodies. The antiserum had a titre of 1 : 2000 in an indirect enzyme-linked immunosorbent assay (ELISA) and reacted specifically in immunoblotting and IPTA-ELISA (indirect plate-trapped antigen (PTA)-ELISA)

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    Abstracts of presentations on plant protection issues at the xth international congress of virology

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