Isolation and characterization of wild yeast from Morus nigra fruits for production of regional alcoholic beverages

La fruta de la morera es no solo conocida por su exquisito sabor, sino también por sus cualidades nutricionales y elevado contenido de compuestos bioactivos. La elaboración de vino de mora es uno de sus múltiples usos. Con el objetivo de controlar el proceso de fermentación espontánea empleado en la elaboración de vinos artesanales, se procedió al aislamiento de levaduras silvestre en el jugo de mora. Se identificó por biología molecular Saccharomyces cerevisiae como especie predominante. El aislado M01-IX se caracterizó por su tolerancia al etanol y por cinética de consumo de carbohidratos. Los resultados muestran que M01-IX es capaz de consumir el 99% del azúcar presente en las condiciones ensayadas y podría ser empleado para producir bebidas fermentadas con un contenido de al menos 5% de alcohol.The mulberry fruit is not only known for its exquisite taste, but also for its nutritional qualities and high content of bioactive compounds. The elaboration of blackberry wine is one of its many uses. In order to control the spontaneous fermentation process used in the production of artisanal wines, wild yeasts were isolated in blackberry juice. Saccharomyces cerevisiae was identified by molecular biology as the predominant species. The M01-IX isolate was characterized by its tolerance to ethanol and by the kinetics of carbohydrate consumption. The results show that M01-IX is capable of consuming 99% of the sugar present under the conditions tested and could be used to produce fermented beverages with a content of at least 5% alcohol.Fil: Ferreyra, Dario Jorge. Universidad Nacional de Misiones; ArgentinaFil: Martina, Pablo F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentin

Skipper-CCD Sensors for the Oscura Experiment: Requirements and Preliminary Tests

Oscura is a proposed multi-kg skipper-CCD experiment designed for a dark matter (DM) direct detection search that will reach unprecedented sensitivity to sub-GeV DM-electron interactions with its 10 kg detector array. Oscura is planning to operate at SNOLAB with 2070 m overburden, and aims to reach a background goal of less than one event in each electron bin in the 2-10 electron ionization-signal region for the full 30 kg-year exposure, with a radiation background rate of 0.01 dru. In order to achieve this goal, Oscura must address each potential source of background events, including instrumental backgrounds. In this work, we discuss the main instrumental background sources and the strategy to control them, establishing a set of constraints on the sensors' performance parameters. We present results from the tests of the first fabricated Oscura prototype sensors, evaluate their performance in the context of the established constraints and estimate the Oscura instrumental background based on these results

Early Science with the Oscura Integration Test

Oscura is a planned light-dark matter search experiment using Skipper-CCDs with a total active mass of 10 kg. As part of the detector development, the collaboration plans to build the Oscura Integration Test (OIT), an engineering test experiment with 10% of the Oscura's total mass. Here we discuss the early science opportunities with the OIT to search for millicharged particles (mCPs) using the NuMI beam at Fermilab. mCPs would be produced at low energies through photon-mediated processes from decays of scalar, pseudoscalar, and vector mesons, or direct Drell-Yan productions. Estimates show that the OIT would be a world-leading probe for low-mass mCPs.Comment: 21 pages, 13 figure

Polycyclic aromatic hydrocarbons (PAHs) in yerba maté (Ilex paraguariensis St. Hil) traditional infusions (mate and tereré)

Este estudio describe la presencia de hidrocarburos aromáticos policíclicos (HAP) en las infusiones tradicionales frías y calientes de yerba mate ( mate y tereré ), mediante el seguimiento del contenido de benz [a] antraceno, criseno, benzo [b] fluoranteno, benzo [k] fluoranteno , benzo [a] pireno, dibenz [ah] antraceno, benzo [ghi] perileno e indeno [1,2,3-cd] pireno (PAH8), que han sido elegidos como indicadores de la presencia y toxicidad de los HAP en los alimentos por la Agencia Europea de Seguridad Alimentaria. El contenido de PAH8 en mate y tereré se determinó mediante cromatografía líquida de alta resolución utilizando detección de fluorescencia (HPLC-FLD). El contenido de PAH8 en infusiones de yerba mate calientes y frías osciló entre 371,2 y 2438,8 ng / L y entre 19,2 y 937,3 ng / L, respectivamente. El contenido de benzo [a] pireno varió entre 37,0 y 373,9 ng / L en infusiones de yerba mate caliente y entre 7,0 y 92,1 ng / L en infusiones de yerba mate fría. Ninguna de las muestras analizadas excedió los criterios de la Organización Mundial de la Salud para el agua potable, ya que el nivel máximo permitido de benzo [a] pireno es de 700 ng / L.This study describes the occurrence of polycyclic aromatic hydrocarbons (PAHs) in traditional yerba maté hot and cold infusions ( mate and tereré), by monitoring the content of benz[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenz[ah]anthracene, benzo[ghi]perylene and indeno[1,2,3-cd]pyrene (PAH8), that have been chosen as indicators for the occurrence and toxicity of PAHs in food by the European Food Safety Agency. PAH8 content in mate and tereré was determined by high performance liquid chromatography using fluorescence detection (HPLC-FLD).PAH8 contents in hot and cold maté infusions ranged from 371.2 to 2438.8 ng/L and from 19.2 to 937.3 ng/L, respectively. Benzo[a]pyrene contents varied between 37.0 and 373.9 ng/L in hot yerba maté infusions and between 7.0 and 92.1 ng/L in cold yerba maté infusions. None of the samples analyzed exceeded the World Health Organization criteria for drinking water, since the maximum level allowed for benzo[a]pyrene is 700 ng/L.Fil: Thea, Ana Eugenia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Ferreyra, Dario Jorge. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Brumovsky, Luis Alberto. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Schmalko Radichowski, Miguel Eduardo. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentin

Chemical composition and larvicidal activity of Ocimum species essential oils from the south of the Paranaense forest (Argentine) against Aedes aegypti (Diptera: Culicidae) larvae

Aedes aegypti plays a predominant role in the transmission of a variety of diseases, such as yellow fever, dengue fever, malaria, Zika and Chikungunya disease. The reduction of chemicals available for vector control due to the increase in mosquito resistance to insecticides and their negative impact on the human health and the environment have driven the focus of the problem towards the identification and evaluation of sustainable alternatives to synthetic insecticides. The essential oils from the leaves of Ocimum campechianum, Ocimum ovatum, and Ocimum selloi were analyzed by GC/MS and GC/FID and investigated for its larvicidal activity against A. aegypti larvae. In the chromatographic analyses showed that the main compounds were elemicin (22.8%), methyl eugenol (33.4%), and methyl eugenol (46.5%), respectively. The susceptibility tested on essential oils was performed according to the World Health Organization protocol. The highest larvicidal potential was displayed by O. ovatum, followed by O. campechianum and O. selloi, with LC50 values of 47.5, 95.5 and 141.2 ppm, respectively. The rapid and efficient larvicidal activity of these three essential oils led us to consider these results as promising for the search of a potentially effective earth-friendly larvicide against the A. aegypti vector.Fil: Martina, Pablo F.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Le Vraux, María Agostina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Ferreyra, Dario Jorge. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Uliana, Roberto Fabian. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Celaya, Liliana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ingeniería Química; ArgentinaFil: Sanchez Gonzalez, Felipa. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Kolb Koslobky, Nicolás. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentin