43 research outputs found
Customization of 3D-Printed Hinged Ankle-Foot Orthosis Based on Kinematic Evaluation from Motion Capture
- Author
- Publication venue
- place:Cham
- Publication date
- 01/01/2023
- Field of study
Commercial hinged foot-ankle orthoses (HAFO) generally do not ensure the alignment between the mechanical and the anatomical joints, causing discomfort and unnatural walking. This paper presents a method to design a customized HAFO modeled on geometrical and kinematic data collected from a healthy 24-year-old woman. The geometry of the shank and foot was acquired through a 3D scanner. The physiological articular rotation axis (AoR) was estimated through helical axis and SARA algorithms. The identified AoR was then used to properly design the orthosis by making particular attention to the hinge position. Finally, the prototype was manufactured with fused deposition modeling technique, and its functionality was validated through gait analysis trials
Vegetarian low-protein diets supplemented with keto analogues: a niche for the few or an option for many?
- Author
- Publication venue
- 'Oxford University Press (OUP)'
- Publication date
- 01/01/2013
- Field of study
SP420LOW PROTEIN DIETS IN CKD: MULTIPLE AND FEASIBLE. A MULTICENTER STUDY (THE TOPI STUDY)
- Author
- Publication venue
- Publication date
- 01/02/2017
- Field of study
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Association of Low-Protein Supplemented Diets with Fetal Growth in Pregnant Women with CKD.
- Author
- Publication venue
- 'American Society of Nephrology (ASN)'
- Publication date
- 01/01/2014
- Field of study
Neurofibromin Deficiency and Extracellular Matrix Cooperate to Increase Transforming Potential through FAK-Dependent Signaling
- Author
- Publication venue
- 'MDPI AG'
- Publication date
- 12/05/2021
- Field of study
Simple SummaryNeurofibromatosis type 1 is a genetic disease that predisposes to tumors of the nervous system, primarily the neurofibroma. Plexiform neurofibromas (Pnfs) are of the greatest concern because of location, size, and frequent progression to malignancy. Although research is making great progress, the lack of in-depth understanding of the molecular mechanisms driving neoplastic progression results in the absence of prognostic indicators and therapeutic targets. We document that cell-cell cooperativity and the dynamics of the extracellular matrix play important roles in the growth and transformation of Pnf cells, directly through the cooperation of RAS and focal adhesion kinase (FAK) signaling. In turn, we found that treatment of Pnf cells with both MEK and FAK inhibitors is effective in abolishing the transforming ability of these cells.Plexiform neurofibromas (Pnfs) are benign peripheral nerve sheath tumors that are major features of the human genetic syndrome, neurofibromatosis type 1 (NF1). Pnfs are derived from Schwann cells (SCs) undergoing loss of heterozygosity (LOH) at the NF1 locus in an NF1(+/-) milieu and thus are variably lacking in the key Ras-controlling protein, neurofibromin (Nfn). As these SCs are embedded in a dense desmoplastic milieu of stromal cells and abnormal extracellular matrix (ECM), cell-cell cooperativity (CCC) and the molecular microenvironment play essential roles in Pnf progression towards a malignant peripheral nerve sheath tumor (MPNST). The complexity of Pnf biology makes treatment challenging. The only approved drug, the MEK inhibitor Selumetinib, displays a variable and partial therapeutic response. Here, we explored ECM contributions to the growth of cells lacking Nfn. In a 3D in vitro culture, NF1 loss sensitizes cells to signals from a Pnf-mimicking ECM through focal adhesion kinase (FAK) hyperactivation. This hyperactivation correlated with phosphorylation of the downstream effectors, Src, ERK, and AKT, and with colony formation. Expression of the GAP-related domain of Nfn only partially decreased activation of this signaling pathway and only slowed down 3D colony growth of cells lacking Nfn. However, combinatorial treatment with both the FAK inhibitor Defactinib (VS-6063) and Selumetinib (AZD6244) fully suppressed colony growth. These observations pave the way for a new combined therapeutic strategy simultaneously interfering with both intracellular signals and the interplay between the various tumor cells and the ECM
Multiple Pregnancies in CKD Patients: An Explosive Mix.
- Author
- Publication venue
- 'American Society of Nephrology (ASN)'
- Publication date
- 01/01/2013
- Field of study
Chronic kidney disease, severe arterial and arteriolar sclerosis and kidney neoplasia: on the spectrum of kidney involvement in MELAS syndrome
- Author
- A Lorenc
- A Mima
- A Nagy
- A Ohno
- B Guéry
- C Bárcena
- C Koszka
- C Yanagihara
- DM Sproule
- EB Ringelstein
- F Kurogouchi
- Federica Fassio
- Federica Neve Vigotti
- Francesco Porpiglia
- G Gasparre
- G Remuzzi
- Gabriella Restagno
- Giorgina Barbara Piccoli
- H Kubota
- H Mochizuki
- HI Cheong
- Isabelle Brocheriou
- J Lu
- JJ Jansen
- JM Gilchrist
- K Abu-Amero
- K Trpkov
- KK Lau
- KR Kaufman
- Laura Davico Bonino
- M Hirano
- M Thambisetty
- M Windpessl
- Martina Ferraresi
- MM Löwik
- MT Nguyen
- MV Yusenko
- N Iwasaki
- O Hotta
- Paola Campisi
- RA Erlandson
- S DiMauro
- S Gobbo
- S Manouvrier
- S Nakamura
- S Sangkhathat
- SD Maxwell
- SK Tickoo
- SK Tickoo
- SR Lederer
- TH Hostetter
- WS An
- ZQ Zhang
- Publication venue
- BioMed Central
- Publication date
- 01/01/2012
- Field of study
<p>Abstract</p> <p>Background</p> <p>MELAS syndrome (MIM ID#540000), an acronym for Mitochondrial Encephalopathy, Lactic Acidosis and Stroke-like episodes, is a genetically heterogeneous mitochondrial disorder with protean manifestations and occasional kidney involvement. Interest in the latter is rising due to the identification of cases with predominant kidney involvement and to the hypothesis of a link between mitochondrial DNA and kidney neoplasia.</p> <p>Case presentation</p> <p>We report the case of a 41-year-old male with full blown MELAS syndrome, with lactic acidosis and neurological impairment, affected by the "classic" 3243A > G mutation of mitochondrial DNA, with kidney cancer. After unilateral nephrectomy, he rapidly developed severe kidney functional impairment, with nephrotic proteinuria. Analysis of the kidney tissue at a distance from the two tumor lesions, sampled at the time of nephrectomy was performed in the context of normal blood pressure, recent onset of diabetes and before the appearance of proteinuria. The morphological examination revealed a widespread interstitial fibrosis with dense inflammatory infiltrate and tubular atrophy, mostly with thyroidization pattern. Vascular lesions were prominent: large vessels displayed marked intimal fibrosis and arterioles had hyaline deposits typical of hyaline arteriolosclerosis. These severe vascular lesions explained the different glomerular alterations including ischemic and obsolescent glomeruli, as is commonly observed in the so-called "benign" arteriolonephrosclerosis. Some rare glomeruli showed focal segmental glomerulosclerosis; as the patient subsequently developed nephrotic syndrome, these lesions suggest that silent ischemic changes may result in the development of focal segmental glomerulosclerosis secondary to nephron loss.</p> <p>Conclusions</p> <p>Nephron loss may trigger glomerular sclerosis, at least in some cases of MELAS-related nephropathy. Thus the incidence of kidney disease in the "survivors" of MELAS syndrome may increase as the support therapy of these patients improves.</p
Associations between depressive symptoms and disease progression in older patients with chronic kidney disease: results of the EQUAL study
- Author
- Adamasco Cupisti
- Adelia Sagliocca
- Alberto Ferraro
- Aleksandra Musiała
- Alessandra Mele
- Alessandro Naticchia
- Alex Còsaro
- Alistair Woodman
- Andrea Ranghino
- Andrea Stucchi
- Andreas Jonsson
- Andreas Schneider
- Angelo Pignataro
- Anita Schrander
- Anke Torp
- Anna McKeever
- Anna Szymczak
- Anna-Lena Blom
- Antonella De Blasio
- Antonello Pani
- Aris Tsalouichos
- Asad Ullah
- Barbara McLaren
- Bastiaan van Dam
- Beate Iwig
- Bellasi Antonio
- Biagio Raffaele Di Iorio
- Björn Rogland
- Boris Perras
- Boukje C. Eveleens Maarse
- Butti Alessandra
- Camille Harron
- Carin Wallquist
- Carl Siegert
- Carla Barrett
- Carlo Gaillard
- Cataldo Abaterusso
- Charles Beerenhout
- Charlotte O'Toole
- Chiara Somma
- Christian Marx
- Christiane Drechsler
- Christina Summersgill
- Christof Blaser
- Christoph Wanner
- Christoph Wanner
- Claudia D'alessandro
- Claudia Emde
- Claudia Torino
- Claudia Zullo
- Claudio Pozzi
- Colin Geddes
- Constantijn Konings
- Cornelis Verburgh
- Cynthia Janmaat
- Daniela Bergamo
- Daniele Ciurlino
- Daria Motta
- Deborah Glowski
- Deborah McGlynn
- Denes Vargas
- Detlef Krieter
- Domenico Russo
- Dunja Fuchs
- Dympna Sands
- Ellen Hoogeveen
- Ellen Irmler
- Emöke Dimény
- Enrico Favaro
- Eva Platen
- Ewelina Olczyk
- Ewout Hoorn
- Federica Vigotti
- Fergus Caskey
- Fergus J. Caskey
- Ferruccio Ansali
- Ferruccio Conte
- Francesca Cianciotta
- Francesca Giacchino
- Francesco Cappellaio
- Francesco Pizzarelli
- Fredrik Sundelin
- Fredrik Uhlin
- Friedo Dekker
- Friedo W. Dekker
- Gaetana Porto
- Gaetano Greco
- Geena Roy
- Giada Bigatti
- Giancarlo Marinangeli
- Gianfranca Cabiddu
- Gillian Hirst
- Giordano Fumagalli
- Giorgia Caloro
- Giorgina Piccoli
- Giovanbattista Capasso
- Giovanni Gambaro
- Giuliana Tognarelli
- Giuseppe Bonforte
- Giuseppe Conte
- Giuseppe Toscano
- Goffredo Del Rosso
- Gunilla Welander
- Hanna Augustyniak-Bartosik
- Hans Schmidt-Gürtler
- Hayley King
- Helen McNally
- Hendrik Schlee
- Henk Boom
- Holger Naujoks
- Houda Masri-Senghor
- Hugh Murtagh
- Hugh Rayner
- Ilona Miśkowiec-Wiśniewska
- Ines Schlee
- Irene Capizzi
- Isabel Bascaran Hernandez
- Ivano Baragetti
- Jacek Manitius
- Jan-Willem Eijgenraam
- Jane Turner
- Jeroen Kooman
- Joachim Beige
- Joanna Pondel
- Joanne Wilcox
- Jocelyn Berdeprado
- Jochen Röthele
- Johannes Boots
- Jonathan Wong
- Joris Rotmans
- Joyce Banda
- Justyna Mazur
- Kai Hahn
- Kamila Jędrzejak
- Katarzyna Nowańska
- Katja Blouin
- Katrin Neumeier
- Kirsteen Jones
- Kirsten Anding-Rost
- Kitty J. Jager
- Kitty Jager
- Knut-Christian Gröntoft
- Lamberto Oldrizzi
- Lesley Haydock
- Liffert Vogt
- Lily Wilkinson
- Loreto Gesualdo
- Lothar Schramm
- Luigi Biancone
- Maarten Raasveld
- Maciej Szymczak
- Maciej Szymczak
- Magdalena Durlik
- Magdalena Krajewska
- Manuela Magnano
- Marc Vervloet
- Marco Ricardi
- Margaret Carmody
- Maria Di Bari
- Maria Laudato
- Maria Luisa Sirico
- Maria Stendahl
- Maria Svensson
- Maria Weetman
- Marie Evans
- Marie Evans Olof Heimburger
- Marjolijn van Buren
- Martin Joinson
- Martina Ferraresi
- Mary Dutton
- Maurizio Postorino
- Merel van Diepen
- Michael Matthews
- Michele Provenzano
- Monika Hopf
- Moreno Malaguti
- Nadja Wuttke
- Neal Morgan
- Nicholas C. Chesnaye
- Nicholas Chesnaye
- Nicola Palmieri
- Nikolaus Frischmuth
- Nina Bleakley
- Olof Heimburger
- Paola Murrone
- Paul Cockwell
- Paul Leurs
- Paul Roderick
- Pauline Voskamp
- Pavlos Kashioulis
- Pawlos Ichtiaris
- Peter Blankestijn
- Petra Kirste
- Petra Schulz
- Phil Mason
- Philip Kalra
- Pietro Cirillo
- Pietro Dattolo
- Pina Acampora
- Rincy Sajith
- Rita Nigro
- Robbert Schouten
- Roberto Boero
- Roberto Scarpioni
- Rosa Sicoli
- Rosella Malandra
- Sabine Aign
- Sabine Cäsar
- Sadie van Esch
- Sally Chapman
- Sandra Biribauer
- Santee Navjee
- Sarah Crosbie
- Sharon Brown
- Sheila Tickle
- Sherin Manan
- Silke Röser
- Silvana Savoldi
- Silvio Bertoli
- Silvio Borrelli
- Siska Boorsma
- Stefan Berger
- Stefan Heidenreich
- Stefan Melander
- Stefania Maxia
- Stefano Maffei
- Stefano Mangano
- Stephanie Palm
- Suresh Mathavakkannan
- Susanne Schwedler
- Sylke Delrieux
- Sylvia Renker
- Sylvia Schättel
- Szyszkowska Dorota
- Teresa Cicchetti
- Teresa Nieszporek
- Theresa Stephan
- Thomas Schmiedeke
- Thomas Weinreich
- Til Leimbach
- Tiziana Rappa
- Tora Almquist
- Torsten Stövesand
- Udo Bahner
- Ulrika Jensen
- Valentina Palazzo
- Walter De Simone
- Wieneke M. Michels
- Willem Jan W. Bos
- Wolfgang Seeger
- Ying Kuan
- Yvette Meuleman
- Zbigniew Heleniak
- Zeynep Aydin
- Łukasz Nowak
- Publication venue
- Publication date
- 01/01/2022
- Field of study
Background Depressive symptoms are associated with adverse clinical outcomes in patients with end-stage kidney disease; however, few small studies have examined this association in patients with earlier phases of chronic kidney disease (CKD). We studied associations between baseline depressive symptoms and clinical outcomes in older patients with advanced CKD and examined whether these associations differed depending on sex. Methods CKD patients (>= 65 years; estimated glomerular filtration rate <= 20 mL/min/1.73 m(2)) were included from a European multicentre prospective cohort between 2012 and 2019. Depressive symptoms were measured by the five-item Mental Health Inventory (cut-off <= 70; 0-100 scale). Cox proportional hazard analysis was used to study associations between depressive symptoms and time to dialysis initiation, all-cause mortality and these outcomes combined. A joint model was used to study the association between depressive symptoms and kidney function over time. Analyses were adjusted for potential baseline confounders. Results Overall kidney function decline in 1326 patients was -0.12 mL/min/1.73 m(2)/month. A total of 515 patients showed depressive symptoms. No significant association was found between depressive symptoms and kidney function over time (P = 0.08). Unlike women, men with depressive symptoms had an increased mortality rate compared with those without symptoms [adjusted hazard ratio 1.41 (95% confidence interval 1.03-1.93)]. Depressive symptoms were not significantly associated with a higher hazard of dialysis initiation, or with the combined outcome (i.e. dialysis initiation and all-cause mortality). Conclusions There was no significant association between depressive symptoms at baseline and decline in kidney function over time in older patients with advanced CKD. Depressive symptoms at baseline were associated with a higher mortality rate in men
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.
- Author
- Abdel-Aziz AK
- Abdelfatah S
- Abdellatif M
- Abdoli A
- Abel S
- Abeliovich H
- Abildgaard MH
- Abudu YP
- Acevedo-Arozena A
- Adamopoulos IE
- Adeli K
- Adolph TE
- Adornetto A
- Aflaki E
- Agam G
- Agarwal A
- Aggarwal BB
- Agnello M
- Agostinis P
- Agrewala JN
- Agrotis A
- Aguilar PV
- Ahmad ST
- Ahmed ZM
- Ahumada-Castro U
- Aits S
- Aizawa S
- Akkoc Y
- Akoumianaki T
- Akpinar HA
- Al-Abd AM
- Al-Akra L
- Al-Gharaibeh A
- Alaoui-Jamali MA
- Alberti S
- Alcocer-Gómez E
- Alessandri C
- Ali M
- Alim Al-Bari MA
- Aliwaini S
- Alizadeh J
- Almacellas E
- Almasan A
- Alonso A
- Alonso GD
- Altan-Bonnet N
- Altieri DC
- Alves da Costa C
- Alves S
- Alzaharna MM
- Amadio M
- Amantini C
- Amaral C
- Ambrosio S
- Amer AO
- Ammanathan V
- An Z
- Andersen SU
- Andrabi SA
- Andrade-Silva M
- Andres AM
- Angelini S
- Ann D
- Anozie UC
- Ansari MY
- Antas P
- Antebi A
- Antón Z
- Anwar T
- Apetoh L
- Apostolova N
- Araki T
- Araki Y
- Arasaki K
- Araya J
- Araújo WL
- Arden C
- Arguelles S
- Arias E
- Arikkath J
- Arimoto H
- Ariosa AR
- Armstrong-James D
- Arnauné-Pelloquin L
- Aroca A
- Arroyo DS
- Arsov I
- Artero R
- Arévalo M-A
- Asaro DML
- Aschner M
- Ashrafizadeh M
- Ashur-Fabian O
- Atanasov AG
- Au AK
- Auberger P
- Auner HW
- Aurelian L
- Autelli R
- Avagliano L
- Aveic S
- Aveleira CA
- Avin-Wittenberg T
- Aydin Y
- Ayton S
- Ayyadevara S
- Azzopardi M
- Baba M
- Backer JM
- Backues SK
- Bae D-H
- Bae O-N
- Bae SH
- Baehrecke EH
- Baek A
- Baek S-H
- Baek SH
- Bagetta G
- Bagniewska-Zadworna A
- Bai H
- Bai J
- Bai X
- Bai Y
- Bairagi N
- Baksi S
- Balazadeh S
- Balbi T
- Baldari CT
- Balduini W
- Ballabio A
- Ballester M
- Balzan R
- Bandopadhyay R
- Banerjee S
- Banerjee S
- Bao Y
- Baptista MS
- Baracca A
- Barbati C
- Bargiela A
- Barilà D
- Barlow PG
- Barmada SJ
- Barreiro E
- Barreto GE
- Bartek J
- Bartel B
- Bartolome A
- Barve GR
- Basagoudanavar SH
- Bassham DC
- Bast RC
- Basu A
- Batoko H
- Batten I
- Baulieu EE
- Baumgarner BL
- Bayry J
- Beale R
- Beau I
- Beaumatin F
- Bechara LRG
- Beck GR
- Beers MF
- Begun J
- Behl C
- Behrends C
- Behrens GMN
- Bei R
- Bejarano E
- Bel S
- Belaid A
- Belgareh-Touzé N
- Bellarosa C
- Belleudi F
- Bello-Morales R
- Belló Pérez M
- Beltran JSDO
- Beltran S
- Benbrook DM
- Bendorius M
- Benitez BA
- Benito-Cuesta I
- Bensalem J
- Berchtold MW
- Berezowska S
- Bergamaschi D
- Bergami M
- Bergmann A
- Berliocchi L
- Berlioz-Torrent C
- Bernard A
- Berthoux L
- Besirli CG
- Besteiro S
- Betin VM
- Beyaert R
- Bezbradica JS
- Bhaskar K
- Bhatia-Kissova I
- Bhattacharya R
- Bhattacharya S
- Bhattacharyya S
- Bhuiyan MS
- Bhutia SK
- Bi L
- Bi X
- Biden TJ
- Bijian K
- Billes VA
- Binart N
- Bincoletto C
- Birgisdottir AB
- Bjorkoy G
- Blanco G
- Blas-Garcia A
- Blasiak J
- Blomgran R
- Blomgren K
- Blum JS
- Boada-Romero E
- Boban M
- Boesze-Battaglia K
- Boeuf P
- Boland B
- Bomont P
- Bonaldo P
- Bonam SR
- Bonfili L
- Bonifacino JS
- Boone BA
- Bootman MD
- Bordi M
- Borner C
- Bornhauser BC
- Borthakur G
- Bosch J
- Bose S
- Botana LM
- Botas J
- Boulanger CM
- Boulton ME
- Bourdenx M
- Bourgeois B
- Bourke NM
- Bousquet G
- Boya P
- Bozhkov PV
- Bozi LHM
- Bozkurt TO
- Brackney DE
- Brand-Saberi B
- Brandts CH
- Braun RJ
- Braus GH
- Bravo-Sagua R
- Bravo-San Pedro JM
- Brest P
- Bringer M-A
- Briones-Herrera A
- Broaddus VC
- Brodersen P
- Brodsky JL
- Brody SL
- Bronson PG
- Bronstein JM
- Brown CN
- Brown RE
- Brum PC
- Brumell JH
- Brunetti-Pierri N
- Bruno D
- Bryson-Richardson RJ
- Bucci C
- Buch S
- Buchan JR
- Buchrieser C
- Buckingham EM
- Budak H
- Budini M
- Bueno M
- Buitrago-Molina LE
- Bultynck G
- Burada F
- Buraschi S
- Burgoyne JR
- Burón MI
- Bustos V
- Butturini E
- Byrd A
- Bánréti Á
- Büttner S
- Cabas I
- Cabrera-Benitez S
- Cadwell K
- Cai J
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- Cai Q
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- Calbet JA
- Caldwell GA
- Caldwell KA
- Call JA
- Calvani R
- Calvo AC
- Calvo-Rubio Barrera M
- Camara NO
- Camonis JH
- Camougrand N
- Campanella M
- Campbell EM
- Campbell-Valois F-X
- Campello S
- Campesi I
- Campos JC
- Camuzard O
- Cancino J
- Candido de Almeida D
- Canesi L
- Caniggia I
- Canonico B
- Cantí C
- Cao B
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- Caramés B
- Carchman EH
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- Cardenas C
- Cardenas L
- Cardoso SM
- Carew JS
- Carle GF
- Carleton G
- Carloni S
- Carmona-Gutierrez D
- Carneiro LA
- Carnevali O
- Carosi JM
- Carra S
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- Carvalho AN
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- Castro-Obregon S
- Catz SD
- Cavadas C
- Cavaliere F
- Cavallini G
- Cavinato M
- Cayuela ML
- Cebollada Rica P
- Cecarini V
- Cecconi F
- Cechowska-Pasko M
- Cenci S
- Ceperuelo-Mallafré V
- Cerqueira JJ
- Cerutti JM
- Cervia D
- Cetintas VB
- Cetrullo S
- Chae H-J
- Chagin AS
- Chai C-Y
- Chakrabarti G
- Chakrabarti O
- Chakraborty T
- Chakraborty T
- Chami M
- Chamilos G
- Chan DW
- Chan ED
- Chan EYW
- Chan H
- Chan HH
- Chan HYE
- Chan MTV
- Chan YS
- Chandra PK
- Chang C
- Chang C-P
- Chang H-C
- Chang K
- Chao J
- Chapman T
- Charlet-Berguerand N
- Chatterjee S
- Chaube SK
- Chaudhary A
- Chauhan S
- Chaum E
- Checler F
- Cheetham ME
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- Chen Y-J
- Chen Y-Q
- Chen Z
- Chen Z
- Chen Z
- Chen Z-H
- Chen ZJ
- Chen ZS
- Cheng H
- Cheng J
- Cheng S-Y
- Cheng W
- Cheng X
- Cheng X-T
- Cheng Y
- Cheng Z
- Cheong H
- Cheong JK
- Chernyak BV
- Cherry S
- Cheung CFR
- Cheung CHA
- Cheung K-H
- Chevet E
- Chi RJ
- Chiang AKS
- Chiaradonna F
- Chiarelli R
- Chiariello M
- Chica N
- Chiocca S
- Chiong M
- Chiou S-H
- Chiramel AI
- Chiurchiù V
- Cho D-H
- Choe S-K
- Choi AMK
- Choi ME
- Choudhury KR
- Chow NS
- Chu CT
- Chua JJE
- Chua JP
- Chung H
- Chung KP
- Chung S
- Chung S-H
- Chung Y-L
- Cianfanelli V
- Ciechomska IA
- Cifuentes M
- Cinque L
- Cirak S
- Cirone M
- Clague MJ
- Clarke R
- Clementi E
- Coccia EM
- Codogno P
- Cohen E
- Cohen MM
- Colasanti T
- Colasuonno F
- Colbert RA
- Colell A
- Coll NS
- Collins MO
- Colombo MI
- Colón-Ramos DA
- Combaret L
- Comincini S
- Cominetti MR
- Consiglio A
- Conte A
- Conti F
- Contu VR
- Cookson MR
- Coombs KM
- Coppens I
- Corasaniti MT
- Cordes N
- Corkery DP
- Cortese K
- Costa MDC
- Costantino S
- Costelli P
- Coto-Montes A
- Crack PJ
- Crespo JL
- Criollo A
- Crippa V
- Cristofani R
- Csizmadia T
- Cuadrado A
- Cui B
- Cui J
- Cui Y
- Cui Y
- Culetto E
- Cumino AC
- Cybulsky AV
- Czaja MJ
- Czuczwar SJ
- D'Adamo S
- D'Amelio M
- D'Arcangelo D
- D'Lugos AC
- D'Orazi G
- da Silva JA
- Dafsari HS
- Dagda RK
- Dagdas Y
- Daglia M
- Dai X
- Dai Y
- Dai Y
- Dal Col J
- Dalhaimer P
- Dalla Valle L
- Dallenga T
- Dalmasso G
- Damme M
- Dando I
- Dantuma NP
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- Álvarez ÉMC
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- Önal G
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- Čolić M
- Đokić J
- Žerovnik E
- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
- Author
- A. -G. Wu
- A. C. Ma
- A. K. Au
- Abdel-Aziz A. K.
- Abdelfatah S.
- Abdellatif M.
- Abdoli A.
- Abel S.
- Abeliovich H.
- Abildgaard M. H.
- Abudu Y. P.
- Acevedo-Arozena A.
- Adamopoulos I. E.
- Adeli K.
- Adolph T. E.
- Adornetto A.
- Aflaki E.
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- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study