Distribution of cases of endemic mycoses according to the Autonomous Community to which the hospital belongs.

<p>The map was obtained from open access website <a href="http://www.d-maps.com/" target="_blank">www.d-maps.com</a>, and it was modified with the software Inkscape.</p

Evolution of cases of endemic mycoses and Spanish population at risk.

<p>*y1 axis: total number of people/trips in every year. *y2 axis: number of total cases in every year of the studied period, 1997–2014.</p

Main clinical and epidemiological characteristics of Chagas disease patients visited at Vall d’Hebron University Hospital from March 2014 to February 2015.

<p>Main clinical and epidemiological characteristics of Chagas disease patients visited at Vall d’Hebron University Hospital from March 2014 to February 2015.</p

Protozoan parasites observed in stool samples of Chagas disease patients.

<p>Protozoan parasites observed in stool samples of Chagas disease patients.</p

Examples of the high-resolution manometric studies in patients with Chagas disease: (A) Normal lower esophageal sphincter pressure and esophageal peristalsis (B) Hypotonic LES and normal peristalsis and (C) Ineffective esophageal motility and hypotonic LES.

<p>LES: lower esophageal sphincter.</p

Technical manometric parameters according to study group.

<p>Technical manometric parameters according to study group.</p

High resolution manometries results according to the Chicago classification in Chagas cohort and control group.

<p>High resolution manometries results according to the Chicago classification in Chagas cohort and control group.</p

Statistical details on the Hidden Markov Model.

Table A in S2 Text. Parameter estimates for M1 and M2 in D1-2 using the Expectation-Maximization algorithm. Fig A in S2 Text. Schematic representation of evaluated M1-M2 Hidden Markov Models. (PDF)</p

Sensitivity analyses for the use of Ct-values compared to interpolated parasite load.

Fig A in S3 Text. Sensitivity analyses for the Barcelona Cohort, D1. Left row panels showing individual trajectories over time among those in D1 with always detectable parasitemia (gray lines) and those alternating detectable/undetectable parasitemia (red lines) when using Ct-values (top), using interpolated parasite loads (as pEq./mL) with approach 1 (middle top), using interpolated parasite loads with approach 2 (middle bottom) and using interpolated values adjusted for sample volume, approach 3 (bottom). Right panels showing the relationship between Ct-values and parasite loads for a given interpolation approach for individuals with always detectable parasitemia (gray dots) and those alternating detectable/undetectable parasitemia (red dots). Fig B in S3 Text. Sensitivity analyses for the E1224-Placebo arm, D2. Left row panels showing individual trajectories over time among those in D2 with always detectable parasitemia (gray lines) and those alternating detectable/undetectable parasitemia (red lines) when using Ct-values (top), using interpolated parasite loads (as pEq./mL) with approach 1 (middle) and using interpolated parasite loads with approach 2 (bottom). Right panels showing the relationship between Ct-values and parasite loads for a given interpolation approach for individuals with always detectable parasitemia (gray dots) and those alternating detectable/undetectable parasitemia (red dots). Log 10 scale is used for the y-axis to facilitate visualization. Fig C in S3 Text. Distribution of Ct-values relative to sample volume in the Barcelona Cohort (PDF)</p

Ct-values for individuals ranked by parasitemia pattern category and mean Ct-value (from highest to lowest).

The red area comprises individuals with alternating detectable/ undetectable parasitemia with >50% timepoints having detectable parasitemia, with the y-axis spanning 0 and the minimum Ct-value within the group. The green area comprises individuals who always have detectable parasitemia, with the y axis showing the Ct-value range for individuals with continuous positive detection and at least one observation above the threshold 1. The orange area comprises individuals with alternating detectable/undetectable parasitemia with <50% timepoints having detectable parasitemia, and the y-axis spanning 40 and the minimum Ct-value within the group (threshold 2).</p